{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T19:32:02Z","timestamp":1793475122976,"version":"4.5.0"},"reference-count":27,"publisher":"Veterinary World","issue":"3","license":[{"start":{"date-parts":[[2019,3,30]],"date-time":"2019-03-30T00:00:00Z","timestamp":1553904000000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,3,30]],"date-time":"2019-03-30T00:00:00Z","timestamp":1553904000000},"content-version":"am","delay-in-days":29,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,3,30]],"date-time":"2019-03-30T00:00:00Z","timestamp":1553904000000},"content-version":"tdm","delay-in-days":29,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet World"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:p>Aim: This study aimed to investigate the occurrence of Marek's disease (MD) in five poultry farms in Malaysia using postmortem examination, histopathology, and polymerase chain reaction (PCR).\n\nMaterials and Methods: Tissue samples were collected from 24 broiler breeder chickens from four commercial broiler breeder farms and six layer chickens from one layer farm. Gross and histopathological examinations and PCR amplification of the gene encoding for avian MD herpesvirus (MDV-1) were conducted.\n\nResults: Gross pathological changes including hepatomegaly, splenomegaly, lymphomatous lesion at the mesentery, oviduct atrophy, and follicular atresia with lymphomatous were observed, whereas diffuse multifocal whitish infiltration of the spleen, neoplastic infiltration in the liver, intrafollicular lymphoid infiltration of the bursa of Fabricius, and lymphomatous tumor at the mesentery were seen on histopathological examinations. Confirmation by PCR showed that a total of 16 (53.33%) samples were positive for avian MDV-1. Although the outbreak involved a much larger number of birds in the respective farms, our investigation was limited based on resource and time frame allocated for the study.\n\nConclusion: The findings from this study help in emphasizing the potential threats of MDV to the poultry industry globally, in general, and in Malaysia, in particular. As the scope of the current study is limited, future studies focusing on MDV pathogenesis, typing, and causes of vaccine failures are recommended.<\/jats:p>","DOI":"10.14202\/vetworld.2019.472-476","type":"journal-article","created":{"date-parts":[[2019,3,30]],"date-time":"2019-03-30T10:06:39Z","timestamp":1553940399000},"page":"472-476","source":"Crossref","is-referenced-by-count":13,"title":["Marek's disease herpesvirus serotype 1 in broiler breeder and layer chickens in Malaysia"],"prefix":"10.14202","volume":"12","author":[{"given":"Iryanti","family":"Othman","sequence":"first","affiliation":[{"name":"Department of Paraclinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, 16100 Pengkalan Chepa, Kota Bharu Kelantan, Malaysia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erkihun","family":"Aklilu","sequence":"additional","affiliation":[{"name":"Department of Paraclinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, 16100 Pengkalan Chepa, Kota Bharu Kelantan, Malaysia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5308","published-online":{"date-parts":[[2019,3,30]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"Van Regenmortel, M.H.V., Fauquet, C.M., Bishop, D.H.L., Carsten, E.B., Estes, M.K., Lemon, S.M., Maniloff, J., Mayo, M., McGeoch, D.J., Pringle, C.R. and Wickner, R.B. (2000) Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses. Academic Press, New York.","DOI":"10.1016\/s0168-1702(01)00352-5"},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Venugopal, N. (2005) Evolution of Marek\u2019s disease-a paradigm for incessant race between the pathogen and the host. Vet. J., 170(2): 175-183.","DOI":"10.1016\/j.tvjl.2004.05.009"},{"key":"ref2","unstructured":"Witter, R.L. and Schat, K.A. (2003) Marek\u2019s disease. In: Saif, Y.M., Barness, H.J., Glisson, J.R., Fadly, A.M., McDougald, L.R. and Swayne, D.E. editor. Diseases of Poultry. 11th ed. Iowa State University Press, Ames, Iowa, USA. p407-465."},{"key":"ref3","unstructured":"OIE. (2010) Manual of Diagnostic and Vaccine for Terrestrial Animals. Version Adopted by the World Assembly of Delegates of the OIE in May 2010. World Health Organization for Animal Health (OIE), Paris, France."},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Baigent, S.J. and Davison, F. (2004) Marek\u2019s disease virus: Biology and life cycle. In: Marek\u2019s Disease: An Evolving Problem. Elsevier Academic Press, London, United Kingdom. p62-77.","DOI":"10.1016\/b978-012088379-0\/50010-4"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Becker, Y., Asher, Y., Tabor, E., Davidson, I., Malkinson, M. and Weisman, Y. (1992) Polymerase chain reaction for differentiation between pathogenic and non-pathogenic serotype 1 Marek's disease virus (MDV) and vaccine viruses of MDV-serotypes 2 and 3. J. Virol. Methods, 40(3): 307-322.","DOI":"10.1016\/0166-0934(92)90089-v"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Davidson, I., Borovskaya, A., Perl, S. and Malkinson, M. (1995) Use of the polymerase chain reaction for the diagnosis of natural infection of chickens and turkeys with Marek's disease virus and reticuloendotheliosis virus. Avian Pathol., 24(1): 69-94.","DOI":"10.1080\/03079459508419050"},{"key":"ref7","unstructured":"Frank, F. (2001) Marek\u2019s disease: History, actual and future perspectives. Lohmann Inform. 2001(25): 1-5."},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Lawhale, N.S., Singh, A., Deka, D., Singh, R. and Verma, R. (2014) Detection of Marek\u2019s disease virus meq gene in feather follicle by loop-mediated isothermal amplification. IOSR J. Agric. Vet. Sci., 7(3): 19-24.","DOI":"10.9790\/2380-07311924"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Payne, L.N. and Venugopal, K. (2010) Neoplastic diseases: Marek\u2019s disease, avian leukosis and reticuloendotheliosis. Rev. Sci. Tech., 19(2): 544-564.","DOI":"10.20506\/rst.19.2.1226"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Gimeno, I.M. (2008) Marek\u2019s disease vaccines: A solution for today but a worry for tomorrow? Vaccine, 26(3): C31-C41.","DOI":"10.1016\/j.vaccine.2008.04.009"},{"key":"ref11","unstructured":"Norimah, A.K., Safiah, M., Jamal, K., Zuhaida, H., Rohida, S., Fatimah, S., Norazlin, S., Poh, B., Kandiah, M., Zalilah, M.S., Wan Manan, W.M., Fatimah, S. and Azmi, M.Y. (2008) Food consumption patterns: Findings from the Malaysian adult nutrition survey (MANS). Malays. J. Nutr., 14(1): 25-39."},{"key":"ref12","unstructured":"Abdel-Latif, M.M. and Khalafalla, I.I. (2005) Detection by PCR of multiple subgroups of avian leukosis virus (ALV) in broilers in Sudan. J. Anim. Vet. Adv., 4(3): 407-413."},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Handberg, K.J., Nielsen, O.L. and J\u00f8rgensen, P.H. (2001) The use of serotype 1-and serotype 3-specific polymerase chain reaction for the detection of Marek\u2019s disease virus in chickens. Avian Pathol., 30(3): 243-249.","DOI":"10.1080\/03079450120054659"},{"key":"ref14","unstructured":"Suresh, P., Rajeswar, J.J., Sukumar, K., Srinivasan, P. and Harikrishnan, T.J. (2013) Incidence of Marek\u2019s disease in vaccinated flocks. J. Food Agric. Vet. Sci., 3(1): 200-202."},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Burnside, J. and Morgan, R. (2011) Emerging roles of chicken and viral microRNAs in avian disease. BMC Proc., 5(4): S2.","DOI":"10.1186\/1753-6561-5-s4-s2"},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Biggs, P.M. and Nair, V. (2012) The long view: 40 years of Marek\u2019s disease research and avian pathology. Avian Pathol., 41(1): 3-9.","DOI":"10.1080\/03079457.2011.646238"},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Islam, A., Harrison, B., Cheetham, B.F., Mahony, T.J., Young, P.L. and Walkden-Brown, S.W. (2004) Differential amplification and quantitation of Marek\u2019s disease viruses using real-time polymerase chain reaction. J. Virol. Methods, 119(2): 103-113.","DOI":"10.1016\/j.jviromet.2004.03.006"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Baigent, S.J., Petherbridge, L.J., Howes, K., Smith, L.P., Currie, R.J.W. and Nair, V. (2005) Absolute quantitation of Marek\u2019s disease virus genome copy number in chicken feather and lymphocyte samples using real-time PCR. J. Virol. Methods, 123(1): 53-64.","DOI":"10.1016\/j.jviromet.2004.08.019"},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Abdul-Careem, M.F., Hunter, B.D., Nagy, E., Read, L.R., Sanei, B., Spencer, J.L. and Sharif, S. 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(2002) The feather tips of commercial chickens are a favorable source of DNA for the amplification of MDV and ALV-J. Avian Pathol., 31(3): 237-240.","DOI":"10.1080\/03079450220136549"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Bumstead, N., Sillibourne, J., Rennie, M., Ross, N. and Davison, F. (1997) Quantification of Marek\u2019s disease virus in chicken lymphocytes using the polymerase chain reaction with fluorescence detection. J. Virol. Methods, 65(1): 75-81.","DOI":"10.1016\/s0166-0934(96)02172-6"},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"Choudat, D., Dambrine, G., Delemotte, B. and Coudert, F. (1996) Occupational exposure to poultry and prevalence of antibodies against Marek\u2019s disease virus and avian leukosis retroviruses. J. Occup. Environ. Med., 53(6): 403-410.","DOI":"10.1136\/oem.53.6.403"},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"Laurent, S., Esnault, E., Dambrine, G., Goudeau, A., Choudat, D. and Rasschaert, D. (2001) Detection of avian oncogenic Marek\u2019s disease herpesvirus DNA in human sera. J. Gen. Virol., 82(1): 233-240.","DOI":"10.1099\/0022-1317-82-1-233"},{"key":"ref26","doi-asserted-by":"publisher","unstructured":"MacLea, K.S. and Cheng, H.H. (2007) The threat of Marek\u2019s disease: Virus is expanding. 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