{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T01:12:50Z","timestamp":1778634770342,"version":"3.51.4"},"reference-count":25,"publisher":"Veterinary World","issue":"7","license":[{"start":{"date-parts":[[2019,7,5]],"date-time":"2019-07-05T00:00:00Z","timestamp":1562284800000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,7,5]],"date-time":"2019-07-05T00:00:00Z","timestamp":1562284800000},"content-version":"am","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,7,5]],"date-time":"2019-07-05T00:00:00Z","timestamp":1562284800000},"content-version":"tdm","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013257","name":"Lembaga Penelitian dan Pengabdian Kepada Masyarakat","doi-asserted-by":"publisher","award":["015\/SP2H\/LT\/DRPM\/II\/2016"],"award-info":[{"award-number":["015\/SP2H\/LT\/DRPM\/II\/2016"]}],"id":[{"id":"10.13039\/501100013257","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet World"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:p>Aim: Infectious coryza is caused by Avibacterium paragallinarum. In Indonesia, this infection results in a 10%-40% decrease in egg production by laying hens. This study was conducted to determine the effectiveness of tetravalent coryza vaccine contained A. paragallinarum bacterin serovars A, B, C2, and C3; strain A-221, B-Spross, C2-Modesto, and C-3-Akko in layers based on antibody titer and clinical signs using a post-challenge test. Materials and Methods: Forty four-week-old Lohmanns strain chickens were used in this study. Forty chickens were divided into four groups for serological and challenge test: Group 1 (unvaccinated and challenged by A. paragallinarum serovar A), Group 2 (unvaccinated and challenged by A. paragallinarum serovar B), Group 3 (vaccinated and challenged by A. paragallinarum serovar A), and Group 4 (vaccinated and challenged by A. paragallinarum serovar B). Vaccination was done using the tetravalent vaccine in oil-emulsion adjuvant contained A. paragallinarum bacterin serovars A, B, C2, and C3; strain A-221, B-Spross, C2-Modesto, and C-3-Akko. Vaccination was performed at day 1 and booster was done at day 14. Blood serum was collected on days 0, 14, and 28 for the hemagglutination-hemagglutination inhibition (HI) test. The challenge test was given at day 29 through intranasal administration using A. paragallinarum serovars A-L2447 and B-L1710 approximately 6\u00d7108 CFU\/mL. Clinical signs were observed for 14 days post-infection. At the end of the study, chickens were euthanized, and pathological features of the infraorbital sinus, facial skin, and trachea were recorded. Results: Data analysis of antibody titers and pathological changes was performed descriptively, while clinical symptom scores were analyzed non-parametrically with the Mann\u2013Whitney U-test using SPSS version 21. At days 14 and 28 post-vaccination, the antibody titer in Group 3 was 5 HI and 20 HI, respectively. However, the antibody titers in Group 4 at 28 days post-vaccination were 0 HI. Clinical observations, the vaccinated groups that were challenged with A. paragallinarum serovars A and B showed clinical symptoms on days 4 and 6 post-infection, namely mild unilateral facial edema and severe bilateral facial edema, respectively. Clinical signs in Groups 3 and 4 were less severe than in Groups 1 and 2 (p&lt;0.05). Pathological examination findings supported clinical observations and serological testing. Conclusion: Tetravalent coryza vaccine in chickens has efficacy to protect against the challenge test of A. paragallinarum serovars A and B isolated from Indonesia.<\/jats:p>","DOI":"10.14202\/vetworld.2019.972-977","type":"journal-article","created":{"date-parts":[[2019,7,5]],"date-time":"2019-07-05T11:25:11Z","timestamp":1562325911000},"page":"972-977","source":"Crossref","is-referenced-by-count":9,"title":["Efficacy of tetravalent coryza vaccine against the challenge of Avibacterium paragallinarum serovars A and B isolates from Indonesia in chickens"],"prefix":"10.14202","volume":"12","author":[{"given":"Agnesia Endang Tri Hastuti","family":"Wahyuni","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dhasia","family":"Ramandani","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Veterinary, Vocational College, Universitas Gadjah Mada, Yogyakarta, Indonesia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinsa Cantya","family":"Prakasita","sequence":"additional","affiliation":[{"name":"Department of Microbiology , Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sitarina","family":"Widyarini","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"5308","published-online":{"date-parts":[[2019,7,5]]},"reference":[{"key":"ref0","unstructured":"Anjaneya, S.D., Singh, K., Dhama, V., Gowthaman and Chawak, M.M. (2013) Pathogenicity study of field isolates of Avibacterium paragallinarum in experimentally infected birds. Indian J. Vet. Pathol., 37(1): 13-17."},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Akhter, S., Ali, M., Das, P.M. and Hossain, M.M. (2013) Isolation and identification of Avibacterium paragallinarum, the causal agent of infectious coryza (IC) from layer chickens in Bangladesh. J. Bangladesh Agric. Univ., 11(1): 87-96.","DOI":"10.3329\/jbau.v11i1.18218"},{"key":"ref2","unstructured":"Durairajan, R., Sharma, M. and Murugan, M.S. (2013) Detection of Avibacterium paragallinarum in commercial poultry and their antibiogram. Tamil Nadu J. Vet. Anim. Sci. Res., 9(5): 332-337."},{"key":"ref3","unstructured":"Patil, V.V., Mishra, D.N. and Mane, D.V. (2016) Isolation, characterization and serological study of Avibacterium paragallinarum field isolates from Indian poultry. J. Anim. Poult. Sci., 5(1): 13-20."},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Blackall, P.J. and Soriano-Vargas, E. (2013) Infectious coryza and related bacterial infections. In: Swayne, D.E., Pattison, M., Mc Mullin, P.F. and Bradbury, J.M. Disease of Poultry. 13th ed. Ch. 20. John Wiley and Sons, India. p859-873.","DOI":"10.1002\/9781119421481.ch20"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Chukiatsiri, K., Sasipreeyajan, J., Blackall, P.J., Yuwatanichsampan, S. and Chansiripornchai, N. (2012) Serovar identification, antimicrobial sensitivity, and virulence of Avibacterium paragallinarum isolated from chicken in Thailand. Avian Dis., 56(2): 359-364.","DOI":"10.1637\/9881-080811-reg.1"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Patil, V., Mishra, D. and Mane, D. (2017) Virulence pattern of Avibacterium paragallinarum isolates studied from Indian field condition. Int. J. Livest. Res., 7(2): 201-207.","DOI":"10.5455\/ijlr.20170209071608"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Poernomo, S., Sutarma , Rafiee, M. and Blackall, P.J. (2000) Characterization of isolates of Haemophilus paragallinarum from Indonesia. Aust. Vet. J., 78(11): 759-762.","DOI":"10.1111\/j.1751-0813.2000.tb10447.x"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Dungu, B., Brett, B., MacDonald, R., Deville, S., Dupuis, L., Theron, J. and Bragg, R.R. (2009) Study on the efficacy and safety of different antigens and oil formulations of infectious coryza vaccines containing an NAD-independent strain of Avibacterium paragallinarum. Onderstepoort J. Vet. Res., 76(3): 299-309.","DOI":"10.4102\/ojvr.v76i3.34"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Chukiatsiri, K., Sasipreeyajan, J., Neramitmansuk, W. and Chansiripornchai, N. (2009) Efficacy of autogenous killed vaccine of Avibacterium paragallinarum. Avian Dis., 55(3): 382-386.","DOI":"10.1637\/8563-121908-reg.1"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Zhao, Q., Sun, Y., Zhang, X., Kong, Y., Xie, Z., Zhu, Y., Zhou, E. and Jiang, S. (2010) Evaluation of two experimental infection models for Avibacterium paragallinarum. Vet. Microbiol., 141(1-2): 68-72.","DOI":"10.1016\/j.vetmic.2009.07.034"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Chukiatsiri, K., Chotinun, S. and Chansiripornchai, N. (2010) An outbreak of Avibacterium paragallinarum serovar B in a Thai layer farm. Thai J. Vet. Med., 40(4): 441-444.","DOI":"10.56808\/2985-1130.2264"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Jacobs, A.A.C., van den Berg, K. and Malo, A. (2003) Efficacy of a new tetravalent coryza vaccine against emerging variant Type B strains. Avian Pathol., 32(3): 265-269.","DOI":"10.1080\/0307945031000097859"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Wambura, P.N. (2010) Preparation and use of autogenous vaccine from Avibacterium paragallinarum (strain Tan 1-05) in layer chickens. Trop. Anim. Health Prod., 42(3): 483-486.","DOI":"10.1007\/s11250-009-9447-z"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"Wahyuni, A.E.T., Tabbu, C.R., Artanto, S., Setiawan, D.C.B. and Rajaguguk, S.I. (2018) Isolation, identification, serotyping of Avibacterium paragallinarum from quails in Indonesia with typical infectious coryza disease symptoms. Vet. World, 11(4): 519-524.","DOI":"10.14202\/vetworld.2018.519-524"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Yuan-Man, H., Shieh, H.K., Wei-Hao, C., Jia-Hsiang, S. and Poa-Chun, C. (2007) Immunogenicity and haemagglutination of recombinant Avibacterium paragallinarum HagA. Vet. Microbiol., 122(3-4): 280-289.","DOI":"10.1016\/j.vetmic.2007.01.014"},{"key":"ref16","unstructured":"Blackall, P.J. (2008) Infectious coryza. In: Louise, D.Z., David, E.S., John, R.G., James, E.P., Wilie, M.R., Mark, W. and Peter, R.W. A Laboratory Manual for the Isolation, Identification, and, Characterization of Avian Pathogens. 5th ed. American Association of Avian Pathologists, Athens, GA. p22-26."},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Paudel, S., Hess, M. and Hess, C. (2017) Coinfection of Avibacterium paragallinarum and Gallibacterium anatis in specific-pathogen-free chickens complicates clinical signs of infectious coryza, which can be prevented by vaccination. Avian Dis., 61(1): 55-63.","DOI":"10.1637\/11481-081016-reg"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Paudel, S., Ruhnau, D., Patricia, W., Liebhart, D., Hess, M. and Hess, C. (2017) Presence of Avibacterium paragallinarum and histopathologic lesions corresponds with clinical signs in a co-infection model with Gallibacterium anatis. Avian Dis., 61(3): 335-340.","DOI":"10.1637\/11609-021317-regr"},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Garcia, A.J., Romo, F., Ortiz, M. and Blackall, P.J. (2008) The vaccination-challenge trial: The gold standard test to evaluate the protective efficacy of infectious coryza vaccines. Avian Pathol., 37(2): 183-186.","DOI":"10.1080\/03079450801929941"},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Garcia, A.J., Angulo, E., Blackall, P.J. and Ortiz, A.M. (2004) The presence of nicotinamide adenine dinucleotide-independent Haemophilus paragallinarum in Mexico. Avian Dis., 48(2): 425-429.","DOI":"10.1637\/7104"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Wang, Y., Hsieh, M.K., Tan, D.Y., Shien, J.H., Ou, S.C., Chen, C.F. and Chang, P.C. (2014) The hemagglutinin of Avibacterium paragallinarum is a trimeric autotransportet adhesin that confers haemagglutination, cell adherence and biofilm formation activities. Vet. Microbiol., 174(3-4): 474-482.","DOI":"10.1016\/j.vetmic.2014.10.013"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"Jacobs, A.A.C. and van der Werf, J. (2000) Efficacy of a commercially available coryza vaccine against challenge with recent South African NAD-independent isolates of Haemophilus paragallinarum in chickens. J. S. Afr. Vet. Assoc., 71(2): 109-110.","DOI":"10.4102\/jsava.v71i2.690"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Ali, M., Hossain, M.S., Akter, S., Khan, M.A.H. and Hossain, M.M. (2013) Pathogenesis of infectious coryza in chickens (Gallus gallus) by Avibacterium paragallinarum isolate of Bangladesh. Agriculturists, 11(1): 39-46.","DOI":"10.3329\/agric.v11i1.15240"},{"key":"ref24","unstructured":"Charoenvisal, N., Chansiripornchai, P. and Chansiripornchai, N. (2017) Efficacy of four commercial infectious coryza vaccines on prevention of Avibacterium paragallinarum serovar A, B, and C infection in Thailand. Pak. Vet. 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