{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:01:15Z","timestamp":1787166075932,"version":"build-2736575974"},"reference-count":27,"publisher":"Veterinary World","license":[{"start":{"date-parts":[[2023,4,8]],"date-time":"2023-04-08T00:00:00Z","timestamp":1680912000000},"content-version":"vor","delay-in-days":7,"URL":"https:\/\/www.veterinaryworld.org\/Vol.16\/April-2023\/6.pdf"}],"funder":[{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["PAPIIT IT201816"],"award-info":[{"award-number":["PAPIIT IT201816"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["225311"],"award-info":[{"award-number":["225311"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet World"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:p>Background and Aim: Brucellosis, paratuberculosis (PTb), and infections caused by small ruminant lentivirus (SRLV), formerly known as caprine arthritis encephalitis virus (CAEV), adversely affect goat production systems. Nonetheless, commonly used diagnostic tests can only determine one analyte at a time, increasing disease surveillance costs, and limiting their routine use. This study aimed to design and validate a multiplex assay for antibody detection against these three diseases simultaneously.\n\nMaterials and Methods: Two recombinant proteins from the SRLV (p16 and gp38), the native hapten of Brucella melitensis, and the paratuberculosis-protoplasmic antigen 3 from Mycobacterium avium subsp. paratuberculosis (MAP) were used to devise and assess a multiplex assay. Conditions for the Luminex\u00ae multiplex test were established and validated by sensitivity, specificity, repeatability, and reproducibility parameters. Cut-off points for each antigen were also established.\n\nResults: The 3-plex assay had high sensitivity (84%) and specificity (95%). The maximum coefficients of variation were 23.8% and 20.5% for negative and positive control samples, respectively. The p16 and gp38 SRLV antigens are 97% and 95%, similar to the CAEV sequence found in GenBank, respectively.\n\nConclusion: The multiplex test can be effectively used for the simultaneous detection of antibodies against SRLV, MAP and B. melitensis in goats.\n\nKeywords: antibody detection, brucellosis, Luminex\u00ae, paratuberculosis, serological test, small ruminant lentivirus.<\/jats:p>","DOI":"10.14202\/vetworld.2023.704-710","type":"journal-article","created":{"date-parts":[[2023,4,8]],"date-time":"2023-04-08T08:02:43Z","timestamp":1680940963000},"page":"704-710","source":"Crossref","is-referenced-by-count":3,"title":["Multiplex assay for the simultaneous detection of antibodies against small ruminant lentivirus, Mycobacterium avium subsp. paratuberculosis, and Brucella melitensis in goats"],"prefix":"10.14202","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5036-4775","authenticated-orcid":false,"given":"H\u00e9ctor D.","family":"N\u00e1jera-Rivera","sequence":"first","affiliation":[{"name":"Department of Reproduction, Veterinary School, UNAM, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6606-8508","authenticated-orcid":false,"given":"Ana D.","family":"Rodr\u00edguez-Cortez","sequence":"additional","affiliation":[{"name":"Department of Reproduction, Veterinary School, UNAM, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7492-9195","authenticated-orcid":false,"given":"Mar\u00eda G.","family":"Anaya-Santill\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Veterinary School, UNAM, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1669-1323","authenticated-orcid":false,"given":"Efr\u00e9n","family":"D\u00edaz-Aparicio","sequence":"additional","affiliation":[{"name":"CENID, INIFAP, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6148-5799","authenticated-orcid":false,"given":"Ariadna V.","family":"Ramos-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Veterinary School, UNAM, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4161-0801","authenticated-orcid":false,"given":"Irlanda J.","family":"Siliceo-Cantero","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Veterinary School, UNAM, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8575-5562","authenticated-orcid":false,"given":"Norma C.","family":"V\u00e1zquez-Franco","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology, IPICYT, San Luis Potosi, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6207-4887","authenticated-orcid":false,"given":"Erik","family":"Nieto-Patl\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Veterinary School, UNAM, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2933-2856","authenticated-orcid":false,"given":"Alejandro","family":"De Las Pe\u00f1as","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology, IPICYT, San Luis Potosi, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8073-7560","authenticated-orcid":false,"given":"Liliana M.","family":"Vald\u00e9s-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Veterinary School, UNAM, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8660-411X","authenticated-orcid":false,"given":"Laura","family":"Cobos-Mar\u00edn","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Veterinary School, UNAM, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5308","published-online":{"date-parts":[[2023,4,8]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"1.\tTajonar, K., D\u00edaz, C.A.L., Ibarra, L.E.S., Chay-Canul, A.J., Gonzalez-Ronquillo, M. and Vargas-Bello-P\u00e9rez, E. 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