{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T20:01:06Z","timestamp":1773518466809,"version":"3.50.1"},"reference-count":28,"publisher":"Veterinary World","license":[{"start":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T00:00:00Z","timestamp":1681862400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T00:00:00Z","timestamp":1681862400000},"content-version":"tdm","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet World"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:p>Background and Aim: Infectious bovine keratoconjunctivitis is the most crucial ophthalmic disease among ruminants worldwide. Moraxella is the bacteria generally associated with this disease and leads to keratitis, conjunctivitis, corneal ulcers, or blindness. Platelet-rich plasma (PRP) effects in corneal ulcers and different ocular superficial diseases in animals and humans are beneficial and enhance rapid healing and improvement, but the effects in infectious keratoconjunctivitis in ruminants are uncertain. This study aimed to examine the effect of PRP on re-epithelization, corneal tissue, clinical signs, and matrix metalloproteinase (MMP) expression in sheep with infectious keratoconjunctivitis.\n\nMaterials and Methods: Eighteen sheep were divided into three groups and subjected to a disease-induction experiment. Group 1 (G1) was administered 1.0 mL PRP subconjunctivally, Group 2 (G2) was administered 1.0 mL PRP subconjunctivally and 50 \u03bcL gentamicin drops, and the control group (CG) was administered 50 \u03bcL saline solution topically every 12 h. Clinical ophthalmologic examination, fluorescein staining, and photography were carried out. Ulcerated areas were measured employing J-Image software. Five and eleven days following the procedure, half of the animals from each group were euthanized, and their corneas were evaluated by histopathology and zymography.\n\nResults: Control Group and G2 epithelialized more rapidly. The CG exhibited fewer clinical signs of ocular disease. In histopathological analysis, in G2, alterations were observed only in the epithelium. The CG and G1 exhibited alterations in the epithelium, stroma, and Descemet\u2019s membrane. In zymography, a decline in MMP-2 expression in the animals treated with PRP was detected. Matrix metalloproteinase-9 was significantly expressed in the animals treated with PRP monotherapy, whereas PRP + gentamicin and CG caused a decrease.\n\nConclusion: Platelet-rich plasma alone did not demonstrate any beneficial effect on re-epithelialization, a decline in clinical signs, tissue alterations, and expression of metalloproteinases. Platelet-rich plasma combined with gentamicin was capable of suppressing MMPs, primarily MMP-9, but do not display positive effects in re-epithelization, reduction of clinical signs, or tissue effects. These outcomes are similar to those discovered in untreated animals, so the use of PRP in patients with infectious keratoconjunctivitis does not offer greater benefits in sheep. Additional research is required to validate the results of PRP use in natural disease presentation.\n\nKeywords: corneal ulcers, experimental model, matrix metalloproteinase, Moraxella spp., pinkeye, platelet-rich plasma.<\/jats:p>","DOI":"10.14202\/vetworld.2023.799-810","type":"journal-article","created":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T07:58:15Z","timestamp":1681891095000},"page":"799-810","update-policy":"https:\/\/doi.org\/10.14202\/vetworld.2022.policies","source":"Crossref","is-referenced-by-count":3,"title":["Effects of platelet-rich plasma on corneal re-epithelization and metalloproteinase expression in the cornea of sheep with experimentally-induced infectious keratoconjunctivitis"],"prefix":"10.14202","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2642-7227","authenticated-orcid":false,"given":"Dunia Yisela Trujillo","family":"Piso","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, Impronta Research Group, Cooperative University of Colombia, Ibagu\u00e9, Tolima, Colombia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2926-6895","authenticated-orcid":false,"given":"M\u00f3nica Yamile Padilla","family":"Barreto","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, Impronta Research Group, Cooperative University of Colombia, Ibagu\u00e9, Tolima, Colombia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5297-1605","authenticated-orcid":false,"given":"Maria del Pilar Sanchez","family":"Bonilla","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, Impronta Research Group, Cooperative University of Colombia, Ibagu\u00e9, Tolima, Colombia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2180-6762","authenticated-orcid":false,"given":"Analorena Cifuentes","family":"Rinc\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, Impronta Research Group, Cooperative University of Colombia, Ibagu\u00e9, Tolima, Colombia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3998-6631","authenticated-orcid":false,"given":"Omar Leonardo Aristiz\u00e1bal","family":"P\u00e1ez","sequence":"additional","affiliation":[{"name":"Department of Animal Health, Small Animal Medicine and Surgery Research Group, University of Tolima, Ibagu\u00e9, Tolima, Colombia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0307-727X","authenticated-orcid":false,"given":"Carlos Augusto","family":"Rengifo","sequence":"additional","affiliation":[{"name":"Department of Animal Health, Small Animal Medicine and Surgery Research Group, University of Tolima, Ibagu\u00e9, Tolima, Colombia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3462-4722","authenticated-orcid":false,"given":"Alexandre Lima","family":"de Andrade","sequence":"additional","affiliation":[{"name":"Department of Clinics, Surgery, and Animal Reproduction, College of Veterinary Medicine, Sao Paulo State University, Aracatuba, SP Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"5308","published-online":{"date-parts":[[2023,4,18]]},"reference":[{"key":"ref0","unstructured":"Turnes, C.G. and Santos, J.R.G. (2016) Ceratoconjuntivite infecciosa bovina. In: Megid, J., Ribeiro, M.G. and Paes, A.C., editors. Doen\u00e7as Infecciosas em Animais de Produ\u00e7\u00e3o e de Companhia. Roca, Rio de Janeiro. p71\u201373."},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Maier, G.U., Davy, J.S., Forero, L.C., Bang, H., Clothier, K. and Angelos, J.A. (2021) Effects of eye patches on corneal ulcer healing and weight gain in stocker steers on pasture: A randomized controlled trial. Transl. Anim. Sci., 5(4): txab162.","DOI":"10.1093\/tas\/txab162"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Karthik, K., Manimaran, K., Mahaprabhu, R. and Shoba, K. (2017) Isolation of Moraxella spp. from cases of keratoconjunctivitis in an organized sheep farm of India. Open J. Vet. Med., 7(10): 138\u2013143.","DOI":"10.4236\/ojvm.2017.710014"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Seid, A. (2019) Review on infectious bovine keratoconjunctivitis and its economic impacts in cattle. Dairy Vet. Sci. J., 9(5): 555774.","DOI":"10.19080\/jdvs.2019.09.555774"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Laronha, H. and Caldeira, J. (2020) Structure and function of human matrix metalloproteinases. Cells, 9(5): 1076.","DOI":"10.3390\/cells9051076"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Kimmitt, B.A., Moore, G.E. and Stiles, J. (2018) Comparison of the efficacy of various concentrations and combinations of serum, ethylenediaminetetraacetic acid, tetracycline, doxycycline, minocycline, and N-acetylcysteine for inhibition of collagenase activity in an in vitro corneal degradation model. Am. J. Vet. Res., 79(5): 555\u2013561.","DOI":"10.2460\/ajvr.79.5.555"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Farghali, H.A., Abdelkader, N.A., Abubakr, H.O., Ramadan, E.S., Khattab, M.S., Salem, N.Y. and Emam, I.A. (2021) Corneal ulcer in dogs and cats: Novel clinical application of regenerative therapy using a subconjunctival injection of autologous platelet-rich plasma. Front. Vet. Sci., 8: 641265.","DOI":"10.3389\/fvets.2021.641265"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Griffith, G.L., Holt, A.W., Eriksson, E., Johnson, A.J. and McDaniel, J.S. (2021) Human platelet lysate delivered via an ocular wound chamber for the treatment of corneal epithelial injuries. Exp. Eye Res., 206: 108493.","DOI":"10.1016\/j.exer.2021.108493"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Acosta, L., Castro, M., Fernandez, M., Oliveres, E., Gomez-Demmel, E. and Tartara, L. (2014) Tratamiento de \u00falceras corneales con plasma rico en plaquetas (Treatment of corneal ulcers with platelet rich plasm). Arch. Soc. Esp. Oftalmol., 89(2): 48\u201352.","DOI":"10.1016\/j.oftal.2013.09.002"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Tanidir, S.T., Yuksel, N., Altintas, O., Yildiz, D.K., Sener, E. and Caglar, Y. (2010) The effect of subconjunctival platelet-rich plasma on corneal epithelial wound healing. Cornea, 29(6): 664\u2013669.","DOI":"10.1097\/ico.0b013e3181c29633"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Merlini, N.B., Fonzar, J.F., Perches, C.S., Sereno, M.G., Souza, V.L., Estanislau, C.A., Rodas, N.R., Ranzani, J.J.T., Maia, L., Padovani, C.R. and Brand\u00e3o, C.V.S. (2014) Use of platelet-rich plasma on corneal ulcers in dogs. Arq. Bras. Med. Vet. Zootec., 66(6): 1742\u20131750.","DOI":"10.1590\/1678-6906"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Vatnikov, Y.A., Erin, I.S., Suleimanov, S.M., Kulikov, E.V., Seleznev, S.B., Lutsay, V.I., Popova, I.A. Strizhakov, A.A. and Vilkovysky, I.F. (2020) Effect of autologous plasma treatment on the cornea regeneration with keratoconjunctivitis sicca in dogs. J. Anim. Health. Prod., 8(1): 1\u20137.","DOI":"10.17582\/journal.jahp\/2020\/8.1.1.7"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Perches, C.S., Pellizzon, C.H., Ranzani, J.J.T., Donatti, C., Padovani, C.R., Merlini, N.B., Fonzar, J.F., Beserra, H.E.O., Rocha, N.S. and Brand\u00e3o, C.V.S. (2015) Express\u00e3o de metaloproteinases de matriz e PCNA em \u00falceras de c\u00f3rnea profundas, induzidas em coelhos, tratadas com plasma rico em plaquetas (Expression of matrix metalloproteinases and PCNA induced deep corneal ulcers in rabbits, treated with platelet rich plasma). Arq. Bras. Vet Med. Zootec., 67(6): 1607\u20131615.","DOI":"10.1590\/1678-4162-8142"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Sakimoto, T., Shoji, J., Yamada, A. and Sawa, M. (2007) Upregulation of matrix metalloproteinase in tear fluid of patients with recurrent corneal erosion. Jpn. J. Ophthalmol., 51(5): 343\u2013346.","DOI":"10.1007\/s10384-007-0455-0"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Pifer, M.A., Maerz, T., Baker, K.C. and Anderson, K. (2014) Matrix metalloproteinase content and activity in low-platelet, low-leukocyte and high-platelet, high-leukocyte platelet-rich plasma (PRP) and the biologic response to PRP by human ligament fibroblasts. Am. J. Sports Med., 42(5): 1211\u20131218.","DOI":"10.1177\/0363546514524710"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Gould, S., Dewell, R., Tofflemire, K., Whitley, R.D., Millman, S.T., Opriessnig, T., Rosenbusch, R., Trujillo, J. and O\u2019Connor, A.M. (2013) Randomized blinded challenge study to assess the association between Moraxella bovoculi and infectious bovine keratoconjunctivitis in dairy calves. Vet. Microbiol., 164(1\u20132): 108\u2013115.","DOI":"10.1016\/j.vetmic.2013.01.038"},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Anitua, E., Zalduendo, M., Troya, M., Padilla, S. and Orive, G. (2015) Leukocyte inclusion within a platelet-rich plasma-derived fibrin scaffold stimulates a more pro-inflammatory environment and alters fibrin properties. PLoS One, 10(3): e0121713.","DOI":"10.1371\/journal.pone.0121713"},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Eldin, H.S., Ibrahim, M. and Elswaidy, N. (2019). A histological and immunohistochemical study of the effect of platelet-rich plasma on a corneal alkali burn in adult male albino rat. Egypt. J. Histol., 42(2): 482\u2013495.","DOI":"10.21608\/ejh.2019.7018.1059"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Zaitoun, A.M.A., Elseleny, M.G.A. and El-Khabaz, K.A.S. (2021) Clinical study on Moraxella-associated infectious keratoconjunctivitis (IKC) of small ruminants. Benha Vet. Med. J., 40(2): 1\u20134.","DOI":"10.21608\/bvmj.2021.80682.1436"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Cie\u015blik-Bielecka, A., Bold, T., Zi\u00f3\u0142kowski, G., Piercha\u0142a, M., Kr\u00f3likowska, A. and Reichert, P. (2018) Antibacterial activity of leukocyte- and platelet-rich plasma: An in vitro study. Biomed. Res. Int., 2018: 9471723.","DOI":"10.1155\/2018\/9471723"},{"key":"ref20","unstructured":"Lugo, G.A.O., Gonz\u00e1lez, A.M.A., Castillo, O.G.J., Garc\u00eda, Q.R., S\u00e1nchez, G.A. and L\u00f3pez, G.H.R. (2018) Uso de plasma rico en factores de crecimiento en pacientes con osteoartritis de rodilla (Use of plasma rich in growth factors in patients with knee osteoartritis) . Acta M\u00e9dica Cent., 12(2): 130\u2013139."},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Kim, H.J., Yeom, J.S., Koh, Y.G., Yeo, J.E., Kang, K.T., Kang, Y.M., Chang, B.S. and Lee, C.K. (2014) Anti-inflammatory effect of platelet-rich plasma on nucleus pulposus cells with response of TNF-\u03b1 and IL-1. J. Orthop. Res., 32(4): 551\u2013556.","DOI":"10.1002\/jor.22532"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"Anitua, E., Zalduendo, M.M., Prado, R., Alkhraisat, M.H. and Orive, G. (2015) Morphogen and pro-inflammatory cytokine release kinetics from PRGF-endoret fibrin scaffolds: Evaluation of the effect of leukocyte inclusion. J. Biomed. Mater. Res. A, 103(3): 1011\u20131020.","DOI":"10.1002\/jbm.a.35244"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Cheng, H., Zhang, J., Li, J., Jia, M., Wang, Y. and Shen, H. (2017) Platelet-rich plasma stimulates angiogenesis in mice which may promote hair growth. Eur. J. Med. Res., 22(1): 39.","DOI":"10.1186\/s40001-017-0278-5"},{"key":"ref24","unstructured":"Fern\u00e1ndez-Ferreiro, A., Gonz\u00e1lez-Barcia, M., Gil-Mart\u00ednez, M., Santiago-Varela, M., Pardo, M., Blanco-M\u00e9ndez, J., Pi\u00f1ero-Ces, A., Lamas, M.J. and Otero-Espinar, F.J. (2016) Evaluaci\u00f3n de la toxicidad ocular in vitro de los colirios fortificados antibi\u00f3ticos elaborados en los servicios de farmacia hospitalaria (Evaluation of in-vitro toxicity of fortified eye drops produced in hospital pharmacy services) . Farm. Hosp., 40(5): 352\u2013370."},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"Kaya, S.G., Inanc-Surer, S., Cakan-Akdogan, G., Oktay, G., Utine, C.A. and Kalyoncu, S. (2021) Roles of matrix metalloproteinases in cornea: A special focus on macular corneal dystrophy. Med. Drug Discov., 11: 100095.","DOI":"10.1016\/j.medidd.2021.100095"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Wang, S., Li, Y., Li, S., Yang, J., Tang, R., Li, X., Li, L. and Fei, J. (2020) Platelet-rich plasma loaded with antibiotics as an affiliated treatment for infected bone defect by combining wound healing property and antibacterial activity. Platelets, 32(4): 479\u2013491.","DOI":"10.1080\/09537104.2020.1759792"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"Piso, D.Y.T., Ribeiro, A.P., Silva, M.L., Guimar\u00e3es, P.J., Morales, A., Martins, B.C., Padua, I.M., Renzo, R., Andrade, A.L., Usc\u00e1tegui, R.R. and Laus, J.L. (2014) Effects of antiproteolytic agents on corneal epithelial viability and matrix metalloproteinase-2 and metalloproteinase-9 activity in alkali-burned corneas of rats. Vet. 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