{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:27:49Z","timestamp":1747196869513,"version":"3.40.5"},"reference-count":33,"publisher":"Veterinary World","license":[{"start":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T00:00:00Z","timestamp":1711324800000},"content-version":"vor","delay-in-days":24,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T00:00:00Z","timestamp":1711324800000},"content-version":"tdm","delay-in-days":24,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet World"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:p>Background and Aim: Ambrosia arborescens Mill. (A. arborescens) is an aromatic plant used in traditional medicine as an anti-inflammatory, anti-tussive, anti-rheumatic, and anti-diarrheal agent. This study aimed to evaluate the effect of A. arborescens Mill. on a Rattus norvegicus var. albinus-induced breast cancer model.\n\nMaterials and Methods: We collected A. arborescens from the province of Julcan, La Libertad Region, Per, and prepared an ethanolic extract using pulverized leaves macerated in 96\u00b0 ethanol for 72 h with magnetic stirring. In the evaluation of anticancer activity, four experimental groups with 10 female rats each were formed: Group I (Control-7,12-dimethylbenz[a] anthracene [DMBA]), which received DMBA (single dose) and physiological saline solution for 4 months, and Groups II, III, and IV, which received DMBA (single dose) and 200, 400, and 600 mg\/kg\/day of the ethanolic extract of A. arborescens, respectively, for 4 months.\n\nResults: The DMBA control group presented histological characteristics of ductal carcinoma in situ with necrotic and inflammatory areas, whereas the A. arborescens extract group showed a decrease in tumor volume and recovery of the ductal duct.\n\nConclusion: Ethanol extract of A. arborescens leaves decreases tumor development in rats with induced breast cancer, and this effect is dose-dependent.\n\nKeywords: 7,12-dimethylbenz[a]anthracene, Ambrosia arborescens, breast carcinoma in situ, cancer, rat.<\/jats:p>","DOI":"10.14202\/vetworld.2024.700-704","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T11:29:43Z","timestamp":1711366183000},"page":"700-704","update-policy":"https:\/\/doi.org\/10.14202\/vetworld.2022.policies","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Ambrosia arborescens Mill. ethanolic extract on breast cancer induced in rats"],"prefix":"10.14202","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9866-2739","authenticated-orcid":false,"given":"Carmen R.","family":"Silva-Correa","sequence":"first","affiliation":[{"name":"Departamento de Farmacolog\u00c3\u00ada, Facultad de Farmacia y Bioqu\u00c3\u00admica, Universidad Nacional de Trujillo, Peru."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3956-3559","authenticated-orcid":false,"given":"Victor E.","family":"Villarreal-La Torre","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Facultad de Farmacia y Bioquimica, Universidad Nacional de Trujillo, Peru."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8075-1059","authenticated-orcid":false,"given":"Gladys E.","family":"Lozano-Ciudad","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Facultad de Farmacia y Bioquimica, Universidad Nacional de Trujillo, Peru."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2763-4399","authenticated-orcid":false,"given":"Ricardo M.","family":"Gomez-Arce","sequence":"additional","affiliation":[{"name":"Departamento de Estadistica, Facultad de Ciencias Fisicas y Matematicas, Universidad Nacional de Trujillo, Peru."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4070-7233","authenticated-orcid":false,"given":"Julio A.","family":"Castaneda-Carranza","sequence":"additional","affiliation":[{"name":"Departamento de Estadistica, Facultad de Ciencias Fisicas y Matematicas, Universidad Nacional de Trujillo, Peru."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4645-6914","authenticated-orcid":false,"given":"Deivy Y.","family":"Dionicio-Rosado","sequence":"additional","affiliation":[{"name":"Departamento de Estadistica, Facultad de Ciencias Fisicas y Matematicas, Universidad Nacional de Trujillo, Peru."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1733-4766","authenticated-orcid":false,"given":"Maria E.","family":"Cotrina-Leon","sequence":"additional","affiliation":[{"name":"Departamento de Matematicas, Facultad de Ciencias Fisicas y Matematicas, Universidad Nacional de Trujillo, Peru."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4582-9547","authenticated-orcid":false,"given":"William A.","family":"Sagastegui-Guarniz","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Facultad de Farmacia y Bioquimica, Universidad Nacional de Trujillo, Peru."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4244-7460","authenticated-orcid":false,"given":"Cesar D.","family":"Gamarra-Sanchez","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Facultad de Farmacia y Bioquimica, Universidad Nacional de Trujillo, Peru."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8731-283X","authenticated-orcid":false,"given":"Jose L.","family":"Cruzado-Razco","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Facultad de Farmacia y Bioquimica, Universidad Nacional de Trujillo, Peru."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"5308","published-online":{"date-parts":[[2024,3,25]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"Liu, Y., Yang, S., Wang, K., Lu, J., Bao, X., Wang, R., Qiu, Y., Wang, T. and Yu, H. (2020) Cellular senescence and cancer: Focusing on traditional Chinese medicine and natural products. Cell Prolif., 53(10): e12894.","DOI":"10.1111\/cpr.12894"},{"key":"ref1","unstructured":"World Health Organization. (2023) Cancer. Available from: https:\/\/www.who.int\/es\/news-room\/fact-sheets\/detail\/cancer. Retrieved on 03-12-2023."},{"key":"ref2","unstructured":"World Health Organization. (2023) The Global Cancer Observatory. Available from https:\/\/gco.iarc.fr. Retrieved on 03-12-2023."},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Hanahan, D. and Weinberg, R. (2011) The hallmarks of cancer: The next generation. Cell, 144(5): 646\u2013674.","DOI":"10.1016\/j.cell.2011.02.013"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Mostacero-Le\u00f3n, J., Pel\u00e1ez-Pel\u00e1ez, F., Alarc\u00f3n-Rojas, N., De la Cruz-Castillo, J., Alva-Calder\u00f3n, R. and Charcape-Ravelo, M. (2019) Plants used for the treatment of cancer expanded in the main markets of the province of Trujillo, Peru, 2016\u20132017. Bol. Latinoam. Caribe Plantas Med. Aromat., 18(1): 81\u201394.","DOI":"10.35588\/blacpma.19.18.1.07"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Afifi, A.M., Saad, A.M., Al-Husseini, M.J., Elmehrath, A.O., Northfelt, D.W. and Sonbol, M.B. (2019) Causes of death after breast cancer diagnosis: A US population-based analysis. C\u00e1ncer, 126(7): 1559\u20131567.","DOI":"10.1002\/cncr.32648"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Barroso-Sousa, R. and Metzger-Filho, O. (2016) Differences between invasive lobular and invasive ductal carcinoma of the breast: Results and therapeutic implications. Ther. Adv. Med. Oncol., 8(4): 261\u2013266.","DOI":"10.1177\/1758834016644156"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Mouabbi, J.A., Hassan, A., Lim, B., Hortobagyi, G.N., Tripathy, D. and Layman, R.M. (2022) Invasive lobular carcinoma: An understudied emergent subtype of breast cancer. Breast Cancer Res. Treat., 193(2): 253\u2013264.","DOI":"10.1007\/s10549-022-06572-w"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Bombonati, A. and Sgroi, D.C. (2011) The molecular pathology of breast cancer progression. J. Pathol., 223(2): 307\u2013317.","DOI":"10.1002\/path.2808"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Cserni, G. (2020) Histological type and typing of breast carcinomas and the WHO classification changes over time. Pathologica, 112(1): 25\u201341.","DOI":"10.32074\/1591-951X-1-20"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Bueno, G.A.M. (2017) Clinical and prognostic characteristics of the molecular subtypes of breast cancer determined by immunohistochemistry. Arequipa, Peru. Rev. Peru. Med. Exp. Salud Publica, 34(3): 472\u2013477.","DOI":"10.17843\/rpmesp.2017.343.2530"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Costa, E., Ferreira-Gon\u00e7alves, T., Chasqueira, G., Cabrita, A.S., Figueiredo, I.V. and Reis, C.P. (2020) Experimental models as refined translational tools for breast cancer research. Sci. Pharm., 2020;88(3): 32.","DOI":"10.3390\/scipharm88030032"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Al-Dhaheri, W.S., Hassouna, I., Al-Salam, S. and Karam, S.M. (2008) Characterization of breast cancer progression in the rat. Ann. N. Y. Acad. Sci., 1138: 121\u2013131.","DOI":"10.1196\/annals.1414.018"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Mu\u00f1oz, D. and Cuca, L. (2016) Cytotoxic compounds of plant origin and their relationship with inhibitory proteins of apoptosis (IAP). Rev. Colomb. Cancerol., 20(3): 124\u2013134.","DOI":"10.1016\/j.rccan.2015.10.002"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"De Terrones, T.C. (2014) Characterization of a spironolactone sesquiterpene \u03b1-metilenica from Ambrosia arborescens Miller and evaluation of their biological activity in Trypanosoma cruzi. Rev. Soc. Qu\u00edm. Per\u00fa, 80(2): 124\u2013135.","DOI":"10.37761\/rsqp.v80i2.157"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Ruiz, C., D\u00edaz, C. and Rojas, R. (2015) Chemical composition of essential oils from 10 Peruvian aromatic plants. Rev. Soc. Qu\u00edm. Per\u00fa, 81(2): 81\u201394.","DOI":"10.37761\/rsqp.v81i2.10"},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"De Leo, M., Saltos, M.B.V., Puente, B.F.N., De Tommasi, N. and Braca, A. (2010) Sesquiterpenes and diterpenes from Ambrosia arborescens. Phytochemistry, 71(7): 804\u2013809.","DOI":"10.1016\/j.phytochem.2010.02.002"},{"key":"ref17","unstructured":"Villagomez, R., Rodrigo, G.C., Collado, I.G., Calzado, M.A., Mu\u00f1oz, E., Akesson, B., Sterner, O., Almanza, G.R. and Dong, D.R. (2013) Multiple anticancer effects of damsin and coronopilin isolated from Ambrosia arborescens on cell cultures. Anticancer Res., 33(9): 3799\u20133805."},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Sotillo, W.S., Villagomez, R., Smiljanic, S., Huang, X., Malakpour, A., Kempengren, S., Rodrigo, G., Almanza, G., Sterner, O. and Oredsson, S. (2017) Anti-cancer stem cell activity of a sesquiterpene lactone isolated from Ambrosia arborescens and of a synthetic derivative. PLoS One, 12(9): e0184304.","DOI":"10.1371\/journal.pone.0184304"},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Arroyo-Acevedo, J., Ch\u00e1vez-Asmat, R.J., Anampa-Guzm\u00e1n, A., Donaires, R. and R\u00e1ez-Gonz\u00e1les, J. (2015) Protective effect of Piper aduncum capsule on DMBA-induced breast cancer in rats. Breast Cancer (Auckl), 9(1): 41\u201348.","DOI":"10.4137\/bcbcr.s24420"},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Arroyo, J., Bonilla, P., R\u00e1ez, E., Barreda, A. and Huam\u00e1n, O. (2010) Bidens pilosa chemoprotective effect on induced breast cancer in rats. An. Fac. Med., 71(3): 153\u2013160.","DOI":"10.15381\/anales.v71i3.88"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Rojas-Armas, J.P., Arroyo-Acevedo, J.L., Palomino-Pacheco, M., Herrera-Calder\u00f3n, O., Ortiz-S\u00e1nchez, J.M., Rojas-Armas, A., Calva, J., Castro-Luna, A. and Hilario-Vargas, J. (2020) The essential oil of Cymbopogon citratus stapt and carvacrol: An approach of the antitumor effect on 7,12-dimethylbenz-[\u03b1]-anthracene (DMBA)-induced breast cancer in female rats. Molecules, 25(14): 3284.","DOI":"10.3390\/molecules25143284"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"Serrano Contreras, A.M. and Su\u00e1rez S\u00e1nchez F.H. (2014) Chemopreventive effects of Morindia Citrifolia Juice (noni) on experimental breast cancer in rats: Preliminary study. Rev. Med. Vet., 27: 41\u201357.","DOI":"10.19052\/mv.3023"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Kerdelhu\u00e9, B., Forest, C. and Coumoul, X. (2016) Dimethyl-benz(a)anthracene: A mammary carcinogen and a neuroendocrine disruptor. Biochim. Open, 3(1): 49\u201355.","DOI":"10.1016\/j.biopen.2016.09.003"},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"Abd-Ellatef, G.E.F., Ahmed, O.M., Abdel-Reheim, E.S. and Abdel-Hamid, A.H.Z. (2017) Ulva lactuca polysaccharides prevent Wistar rat breast carcinogenesis through the augmentation of apoptosis, enhancement of antioxidant defense system, and suppression of inflammation. Breast Cancer (Dove Med Press), 27(9): 67\u201383.","DOI":"10.2147\/bctt.s125165"},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"Hamza, A.A., Khasawneh, M.A., Elwy, H.M., Hassanin, S.O., Elhabal, S.F. and Fawzi, N.M. (2022) Salvadora persica attenuates DMBA-induced mammary cancer through downregulation oxidative stress, estrogen receptor expression and proliferation and augmenting apoptosis. Biomed. Pharmacother., 147(1): 112666.","DOI":"10.1016\/j.biopha.2022.112666"},{"key":"ref26","doi-asserted-by":"publisher","unstructured":"De Oliveira, K.D., Avanzo, G.U., Tedardi, M.V., Rangel, M.M.M., Avanzo, J.L., Fukumasu, H., Rao, K.V.K., Sinhorini, I.L. and Dagli, M.L.Z. (2015) Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB\/c mice: New facts. Braz. J. Vet. Res. Anim. Sci., 52(2): 125\u2013133.","DOI":"10.11606\/issn.1678-4456.v52i2p125-133"},{"key":"ref27","doi-asserted-by":"publisher","unstructured":"Youssef, S.S.M., Ibrahim, N.K., El-Sonbaty, S.M. and El-Din Ezz, M.K. (2022) Rutin suppresses DMBA carcinogenesis in the breast through modulating IL-6\/NF-\u03baB, SRC1\/HSP90 and ER-\u03b1. Nat. Prod. Commun., 17(9): 1\u201311.","DOI":"10.1177\/1934578x221118213"},{"key":"ref28","doi-asserted-by":"publisher","unstructured":"Minari, J.B., Ogar, G.O. and Bello, A.J. (2016) Antiproliferative potential of aqueous leaf extract of Mucuna pruriens on DMBA-induced breast cancer in female albino rats. Egypt. J. Med. Hum. Genet., 17(4): 331\u2013343.","DOI":"10.1016\/j.ejmhg.2015.12.008"},{"key":"ref29","doi-asserted-by":"publisher","unstructured":"Kov\u00e1cs, B., Hohmann, J., Csupor-L\u00f6ffler, B., Kiss, T. and Csupor, D. (2022) A comprehensive phytochemical and pharmacological review on sesquiterpenes from the genus Ambrosia. Heliyon, 8(7): e09884.","DOI":"10.1016\/j.heliyon.2022.e09884"},{"key":"ref30","doi-asserted-by":"publisher","unstructured":"Dhyani, P., Sati, P., Sharma, E., Attri, D.C., Bahukhandi, A., Tynybekov, B., Szopa, A., Sharifi-Rad, J., Calina, D., Suleria, H.A.R. and Cho, W.C. (2022) Sesquiterpenoid lactones as potential anti-cancer agents: An update on molecular mechanisms and recent studies. Cancer Cell Int., 22(1): 305.","DOI":"10.1186\/s12935-022-02721-9"},{"key":"ref31","doi-asserted-by":"publisher","unstructured":"Lozano, M., Soria, W., Almanza, G.R., Manner, S., Oredsson, S., Villagomez, R. and Sterner, O. (2019) Cytotoxicity of new damsin derivatives in breast cancer cells. J. Pharm. Drug Dev., 1(2): 22\u201336.","DOI":"10.14302\/issn.2328-0182.japst-19-2759"},{"key":"ref32","doi-asserted-by":"publisher","unstructured":"Hendrayani, S.F., Al-Harbi, B., Al-Ansari, M.M., Silva, G. and Aboussekhra, A. (2016) The inflammatory\/cancer-related IL-6\/STAT3\/NF-\u03baB positive feedback loop includes AUF1 and maintains the active state of breast myofibroblasts. Oncotarget, 7(27): 41974\u201341985.","DOI":"10.18632\/oncotarget.9633"}],"container-title":["Veterinary World"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.veterinaryworld.org\/Vol.17\/March-2024\/22.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T11:30:12Z","timestamp":1711366212000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.veterinaryworld.org\/Vol.17\/March-2024\/22.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":33,"URL":"https:\/\/doi.org\/10.14202\/vetworld.2024.700-704","relation":{},"ISSN":["2231-0916","0972-8988"],"issn-type":[{"type":"electronic","value":"2231-0916"},{"type":"print","value":"0972-8988"}],"subject":[],"published":{"date-parts":[[2024,3]]},"assertion":[{"value":"2024-12-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-3-4","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-3-25","order":2,"name":"published_online","label":"Published Online","group":{"name":"publication_history","label":"Publication History"}}]}}