{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:04:04Z","timestamp":1785423844110,"version":"3.56.0"},"reference-count":54,"publisher":"Veterinary World","license":[{"start":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T00:00:00Z","timestamp":1769817600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. World"],"published-print":{"date-parts":[[2026,1,31]]},"abstract":"<jats:p>Background and Aim: Clostridium perfringens is a major enteric pathogen of goats, capable of producing multiple toxins and harboring diverse antimicrobial resistance (AMR) determinants. The coexistence of toxin diversity and AMR complicates disease control and poses risks to animal health and antimicrobial stewardship. This study aimed to characterize toxin genotypes, phenotypic AMR patterns, and associated resistance genes in C. perfringens isolates obtained from healthy, diseased, and deceased goats in Jiangsu Province, China. \n\nMaterials and Methods: A total of 404 samples were collected from goats between April 2021 and April 2022, including feces from healthy animals, rumen contents from slaughtered goats, and intestinal contents and visceral tissues from diseased or deceased goats. Isolation and identification of C. perfringens were performed using anaerobic culture and 16S rRNA gene sequencing. Toxin genotyping targeting major toxin genes was conducted by polymerase chain reaction (PCR). Antimicrobial susceptibility was assessed using the Kirby\u2013Bauer disk diffusion method, and resistance genes were detected by PCR. Hierarchical clustering was used to explore relationships between toxinotypes and AMR gene profiles. \n\nResults: Ninety-four C. perfringens isolates were recovered (23.3%). Toxinotype A predominated overall (61.7%) and was dominant among healthy goats, whereas toxinotypes D (52.9%), C (14.7%), and F (14.7%) were more frequently detected in diseased or deceased animals. High phenotypic resistance was observed to aminoglycosides, including kanamycin (72.3%), neomycin (66.0%), and gentamicin (58.5%), as well as trimethoprim\u2013sulfamethoxazole (61.7%). All isolates remained susceptible to vancomycin, with low resistance to \u03b2-lactams. Resistance genes associated with aminoglycosides, sulfonamides, tetracyclines, quinolones, and lincosamides were widely distributed. Notably, the quinolone resistance gene qnrS and tetracycline resistance genes tetA(P) and tetB(P) were significantly more prevalent in isolates from diseased goats, particularly toxinotype D. \n\nConclusion: Goat-associated C. perfringens in Jiangsu Province exhibits substantial toxin diversity and a high burden of AMR, with distinct differences between healthy and diseased animals. These findings underscore the need for continuous molecular surveillance, rational antimicrobial use, and integrated control strategies to mitigate risks to goat health and productivity within a One Health framework. \n\nKeywords: antimicrobial resistance, Clostridium perfringens, goats, molecular epidemiology, One Health, PCR detection, toxin genotyping, veterinary microbiology.<\/jats:p>","DOI":"10.14202\/vetworld.2026.409-421","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T17:17:19Z","timestamp":1770052639000},"page":"409","update-policy":"https:\/\/doi.org\/10.14202\/vetworld.2022.policies","source":"Crossref","is-referenced-by-count":0,"title":["Toxin genotypes and antimicrobial resistance profiles of Clostridium perfringens isolated from healthy and diseased goats in Jiangsu Province, China"],"prefix":"10.14202","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3194-2636","authenticated-orcid":false,"given":"Zibei","family":"Huang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Yangzhou University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3656-2467","authenticated-orcid":false,"given":"Siyuan","family":"Su","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Yangzhou University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5637-8431","authenticated-orcid":false,"given":"Haiyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Vocational College of Agriculture and Animal Husbandry, Taizhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9447-3666","authenticated-orcid":false,"given":"Jinlin","family":"Huang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Yangzhou University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2560-1796","authenticated-orcid":false,"given":"Wenbo","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Yangzhou University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5308","published-online":{"date-parts":[[2026,1,31]]},"reference":[{"key":"ref0","doi-asserted-by":"crossref","unstructured":"1.\tHatheway C. L. Toxigenic clostridia. Clin. Microbiol. Rev., 1990;3(1):66\u201398.","DOI":"10.1128\/CMR.3.1.66"},{"key":"ref1","doi-asserted-by":"crossref","unstructured":"2.\tMehdizadeh Gohari I., Navarro M. A., Li J., Shrestha A., Uzal F., McClane B. A. Pathogenicity and virulence of Clostridium perfringens. Virulence, 2021;12(1):723\u2013753.","DOI":"10.1080\/21505594.2021.1886777"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"3.\tDo D. T., Ngo T. T. N., Vu H. D., Duong N. M., Traiyarach S., Lai D. C., Escuder J. M. Presence of toxin-producing bacterial pathogens and associated risk factors in neonatal diarrhea of piglets on commercial sow farms in Vietnam. Vet. World, 2025;18(5):1288\u20131296.","DOI":"10.14202\/vetworld.2025.1288-1296"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"4.\tSaadh M. J., Lafi F. F., Dahadha A. A., Albannan M. S. Immunogenicity of a newly developed vaccine against Clostridium perfringens alpha-toxin in rabbits and cattle. Vet. World, 2022;15(7):1617\u20131623.","DOI":"10.14202\/vetworld.2022.1617-1623"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"5.\tTresha A. O., Arif M., Islam S. S., Haque A. K. M. Z., Rahman M. T., Kabir S. M. L. Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh. Vet. World, 2021;14(10):2809\u20132816.","DOI":"10.14202\/vetworld.2021.2809-2816"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"6.\tJohnson S., Skinner A. M., Lostutter C., Duke T., Posthaus H. Enteritis necroticans and Clostridium perfringens type C: Epidemiological and pathological findings over the past 20 years. PLoS Negl. Trop. Dis., 2025;19(2):e0012836.","DOI":"10.1371\/journal.pntd.0012836"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"7.\tMorrell E. L., Navarro M. A., Garcia J. P., Beingesser J., Uzal F. A. Intestinal pathology in goats challenged with Clostridium perfringens type D strain CN1020 wild-type and its genetically modified derivatives. Vet. Pathol., 2025;62(1):53\u201363.","DOI":"10.1177\/03009858241273122"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"8.\tFeng H., Wu K., Yuan Y., Fang M., Wang J., Li R., Zhang R., Wang X., Ye D., Yang Z. Genomic analysis of Clostridium perfringens type D isolates from goat farms. Vet. Microbiol., 2024;294:110105.","DOI":"10.1016\/j.vetmic.2024.110105"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"9.\tSantos R. A. N. D., Abdel-Nour J., McAuley C., Moore S. C., Fegan N., Fox E. M. Clostridium perfringens associated with dairy farm systems show diverse genotypes. Int. J. Food Microbiol., 2022;382:109933.","DOI":"10.1016\/j.ijfoodmicro.2022.109933"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"10.\tGrenda T., Jarosz A., Sapa\u0142a M., Grenda A., Patyra E., Kwiatek K. Clostridium perfringens\u2014Opportunistic foodborne pathogen, its diversity and epidemiological significance. Pathogens, 2023;12(6):768.","DOI":"10.3390\/pathogens12060768"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"11.\tRood J. I., Adams V., Lacey J., Lyras D., McClane B. A., Melville S. B., Moore R. J., Popoff M. R., Sarker M. R., Songer J. G., Uzal F. A., Van Immerseel F. Expansion of the Clostridium perfringens toxin-based typing scheme. Anaerobe, 2018;53:5\u201310.","DOI":"10.1016\/j.anaerobe.2018.04.011"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"12.\tMada T., Ochi K., Okamoto M., Takamatsu D. First genomic analysis of a Clostridium perfringens strain carrying both the cpe and netB genes and proposal of an amended toxin-based typing scheme. Front. Microbiol., 2025;16:1580271.","DOI":"10.3389\/fmicb.2025.1580271"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"13.\tJohnston M. D., Whiteside T. E., Allen M. E., Kurtz D. M. Toxigenic profile of Clostridium perfringens strains isolated from natural ingredient laboratory animal diets. Comp. Med., 2022;72(1):50\u201358.","DOI":"10.30802\/AALAS-CM-22-000013"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"14.\tLow K. E., Smith S. P., Abbott D. W., Boraston A. B. The glycoconjugate-degrading enzymes of Clostridium perfringens: Tailored catalysts for breaching the intestinal mucus barrier. Glycobiology, 2021;31(6):681\u2013690.","DOI":"10.1093\/glycob\/cwaa050"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"15.\tWessely V., Suchodolski J. S., Cavasin J. P., Holz M., Busch-Hahn K., Unterer S. Prevalence of Clostridium perfringens encoding the netF toxin gene in dogs with acute and chronic gastrointestinal diseases. Pets, 2025;2(1):9.","DOI":"10.3390\/pets2010009"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"16.\tYoshida T., Monma C., Ninomiya Y., Takiguchi S., Fujita S., Uchida Y., Sakoda N., Karginov V. A., Kishikawa J. I., Yamada T., Kawano R., Tsuge H. Serine clamp of Clostridium perfringens binary toxin BECb (CPILEb)-pore confers cytotoxicity and enterotoxicity. Commun. Biol., 2025;8(1):1102.","DOI":"10.1038\/s42003-025-08519-5"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"17.\tYonogi S., Matsuda S., Kawai T., Yoda T., Harada T., Kumeda Y., Gotoh K., Hiyoshi H., Nakamura S., Kodama T., Iida T. BEC, a novel enterotoxin of Clostridium perfringens found in human clinical isolates from acute gastroenteritis outbreaks. Infect. Immun., 2014;82(6):2390\u20132399.","DOI":"10.1128\/IAI.01759-14"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"18.\tAlves G. G., Machado de \u00c1vila R. A., Ch\u00e1vez-Ol\u00f3rtegui C. D., Lobato F. C. Clostridium perfringens epsilon toxin: The third most potent bacterial toxin known. Anaerobe, 2014;30:102\u2013107.","DOI":"10.1016\/j.anaerobe.2014.08.016"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"19.\tAlves G. G., Machado de \u00c1vila R. A., Ch\u00e1vez-Ol\u00f3rtegui C. D., Lobato F. C. Prevalence and antimicrobial resistance patterns of Clostridium perfringens from healthy broiler chickens. One Health, 2025;21:101156.","DOI":"10.1016\/j.onehlt.2025.101156"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"20.\tMora Z. V., Mac\u00edas-Rodr\u00edguez M. E., Arratia-Quijada J., Gonzalez-Torres Y. S., Nu\u00f1o K., Villarruel-L\u00f3pez A. Clostridium perfringens as foodborne pathogen in broiler production: Pathophysiology and potential strategies for controlling necrotic enteritis. Animals, 2020;10(9):1718.","DOI":"10.3390\/ani10091718"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"21.\tAnju K., Karthik K., Divya V., Mala Priyadharshini M. L., Sharma R. K., Manoharan S. Toxinotyping and molecular characterization of antimicrobial resistance in Clostridium perfringens isolated from different sources of livestock and poultry. Anaerobe, 2021;67:102298.","DOI":"10.1016\/j.anaerobe.2020.102298"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"22.\tNazki S., Wani S. A., Parveen R., Ahangar S. A., Kashoo Z. A., Hamid S., Dar Z. A., Dar T. A., Dar P. A. Isolation, molecular characterization, and prevalence of Clostridium perfringens in sheep and goats of Kashmir Himalayas. Vet. World, 2017;10(12):1501\u20131507.","DOI":"10.14202\/vetworld.2017.1501-1507"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"23.\tZhu X., Huang Y., Shi Y., Gao X., Chen D., Liu C., Cao S., Xue X., Li Y. Comparative genomic analysis of food-animal-derived and human-derived Clostridium perfringens isolates from markets in Shandong, China. Front. Microbiol., 2025;16:1543511.","DOI":"10.3389\/fmicb.2025.1543511"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"24.\tTitouche Y., Akkou M., Campa\u00f1a-Burguet A., Gonz\u00e1lez-Azcona C., Djaoui Y., Mechoub D., Fatihi A., Bouchez P., Bouhier L., Houali K., Nia Y., Torres C., Hennekinne J. A. Phenotypic and genotypic characterization of Staphylococcus aureus isolated from nasal samples of healthy dairy goats in Algeria. Pathogens, 2024;13(5):408.","DOI":"10.3390\/pathogens13050408"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"25.\tWu K., Feng H., Ma J., Wang B., Feng J., Zhang H., Jiang Y., Li R., Wang J., Yang Z. Prevalence, toxin-typing and antimicrobial susceptibility of Clostridium perfringens in sheep with different feeding modes from Gansu and Qinghai provinces, China. Anaerobe, 2022;73:102516.","DOI":"10.1016\/j.anaerobe.2022.102516"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"26.\tZhong J. X., Zheng H. R., Wang Y. Y., Bai L. L., Du X. L., Wu Y., Lu J. X. Molecular characteristics and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021. Front. Microbiol., 2023;14:1195083.","DOI":"10.3389\/fmicb.2023.1195083"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"27.\tEjaz H., Qamar M. U., Junaid K., Younas S., Taj Z., Bukhari S. N. A., Abdalla A. E., Abosalif K. O. A., Ahmad N., Saleem Z., Salem E. H. M. The molecular detection of carbapenemases in Acinetobacter calcoaceticus\u2013baumannii complex. Antibiotics, 2022;11(9):1168.","DOI":"10.3390\/antibiotics11091168"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"28.\tSoge O. O., Tivoli L. D., Meschke J. S., Roberts M. C. A conjugative macrolide resistance gene, mef(A), in environmental Clostridium perfringens carrying multiple macrolide and\/or tetracycline resistance genes. J. Appl. Microbiol., 2009;106(1):34\u201340.","DOI":"10.1111\/j.1365-2672.2008.03960.x"},{"key":"ref28","doi-asserted-by":"crossref","unstructured":"29.\tWahdan A., Elhaig M. M. Epidemiology and diagnostic accuracy of Clostridium perfringens toxins in the intestinal contents of camels, sheep, and cattle: A cross-sectional study in Dakahlia governorate, Egypt. Trop. Anim. Health Prod., 2024;56(6):205.","DOI":"10.1007\/s11250-024-04034-7"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"30.\tCamargo A., Ram\u00edrez J. D., Kiu R., Hall L. J., Mu\u00f1oz M. Pathogenic mechanisms of Clostridium perfringens toxins and virulence factors. Emerg. Microbes Infect., 2024;13(1):2341968.","DOI":"10.1080\/22221751.2024.2341968"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"31.\tGarcia J. P., Beingesser J., Fisher D. J., Sayeed S., McClane B. A., Posthaus H., Uzal F. A. The effect of Clostridium perfringens type C strain CN3685 and its isogenic beta toxin null mutant in goats. Vet. Microbiol., 2012;157(3\u20134):412\u2013419.","DOI":"10.1016\/j.vetmic.2012.01.005"},{"key":"ref31","unstructured":"32.\tAlimolaei M., Shamsaddini Bafti M. Isolation and molecular characterization of Clostridium perfringens toxinotypes F and G in diarrhoeic sheep (Ovis aries) flocks in southeast Iran. Arch. Razi Inst., 2023;78(3):1159\u20131168."},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"33.\tForti K., Ferroni L., Pellegrini M., Cruciani D., De Giuseppe A., Crotti S., Papa P., Maresca C., Severi G., Marenzoni M. L., Cagiola M. Molecular characterization of Clostridium perfringens strains isolated in Italy. Toxins, 2020;12(10):650.","DOI":"10.3390\/toxins12100650"},{"key":"ref33","doi-asserted-by":"crossref","unstructured":"34.\tBryan L. E., Kowand S. K., Van Den Elzen H. M. Mechanism of aminoglycoside resistance in anaerobic bacteria: Clostridium perfringens and Bacteroides fragilis. Antimicrob. Agents Chemother., 1979;15(1):7\u201313.","DOI":"10.1128\/AAC.15.1.7"},{"key":"ref34","doi-asserted-by":"crossref","unstructured":"35.\tBush K., Bradford P. A. Beta-lactams and beta-lactamase inhibitors: An overview. Cold Spring Harb. Perspect. Med., 2016;6(8):a025247.","DOI":"10.1101\/cshperspect.a025247"},{"key":"ref35","doi-asserted-by":"crossref","unstructured":"36.\tNimnoi P., Pongsilp N. Identification, characterization, and virulence gene expression of marine enterobacteria in the upper Gulf of Thailand. Microorganisms, 2022;10(3):511.","DOI":"10.3390\/microorganisms10030511"},{"key":"ref36","unstructured":"37.\tNord C. E., Olsson-Liljequist B. Antimicrobial susceptibility of anaerobic bacteria in Sweden in 1983. Scand. J. Infect. Dis. Suppl., 1984;43:44\u201349."},{"key":"ref37","doi-asserted-by":"crossref","unstructured":"38.\tCastrignan\u00f2 E., Yang Z., Feil E. J., Bade R., Castiglioni S., Causanilles A., Gracia-Lor E., Hernandez F., Pl\u00f3sz B. G., Ramin P., Rousis N. I., Ryu Y., Thomas K. V., de Voogt P., Zuccato E., Kasprzyk-Hordern B. Enantiomeric profiling of quinolones and quinolone resistance gene qnrS in European wastewaters. Water Res., 2020;175:115653.","DOI":"10.1016\/j.watres.2020.115653"},{"key":"ref38","doi-asserted-by":"crossref","unstructured":"39.\tAlzahrani O. M., Fayez M., Alswat A. S., Alkafafy M., Mahmoud S. F., Al-Marri T., Almuslem A., Ashfaq H., Yusuf S. Antimicrobial resistance, biofilm formation, and virulence genes in Enterococcus species from small backyard chicken flocks. Antibiotics, 2022;11(3):380.","DOI":"10.3390\/antibiotics11030380"},{"key":"ref39","doi-asserted-by":"crossref","unstructured":"40.\tKhalifa H. O., Oreiby A., Abd El-Hafeez A. A., Abd El Latif A., Okanda T., Kato Y., Matsumoto T. High \u03b2-lactam and quinolone resistance of Enterobacteriaceae from the respiratory tract of sheep and goats with respiratory disease. Animals, 2021;11(8):2258.","DOI":"10.3390\/ani11082258"},{"key":"ref40","doi-asserted-by":"crossref","unstructured":"41.\tJacoby G. A., Strahilevitz J., Hooper D. C. Plasmid-mediated quinolone resistance. Microbiol. Spectr., 2014;2(5).","DOI":"10.1128\/microbiolspec.PLAS-0006-2013"},{"key":"ref41","doi-asserted-by":"crossref","unstructured":"42.\tLee S., Park N., Yun S., Hur E., Song J., Lee H., Kim Y., Ryu S. Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019. Gut Pathog., 2021;13(1):35.","DOI":"10.1186\/s13099-021-00431-7"},{"key":"ref42","doi-asserted-by":"crossref","unstructured":"43.\tJacoby G. A., Hooper D. C. Phylogenetic analysis of chromosomal determined qnr and related proteins. Antimicrob. Agents Chemother., 2013;57(4):1930\u20131934.","DOI":"10.1128\/AAC.02080-12"},{"key":"ref43","doi-asserted-by":"crossref","unstructured":"44.\tPavelquesi S. L. S., de Oliveira Ferreira A. C. A., Rodrigues A. R. M., de Souza Silva C. M., Orsi D. C., da Silva I. C. R. Presence of tetracycline and sulfonamide resistance genes in Salmonella spp.: Literature review. Antibiotics, 2021;10(11):1314.","DOI":"10.3390\/antibiotics10111314"},{"key":"ref44","doi-asserted-by":"crossref","unstructured":"45.\tPark M., Rooney A. P., Hecht D. W., Li J., McClane B. A., Nayak R., Paine D. D., Rafii F. Phenotypic and genotypic characterization of tetracycline and minocycline resistance in Clostridium perfringens. Arch. Microbiol., 2010;192(10):803\u2013810.","DOI":"10.1007\/s00203-010-0605-5"},{"key":"ref45","doi-asserted-by":"crossref","unstructured":"46.\tDa Silva G. J., Mendon\u00e7a N. Association between antimicrobial resistance and virulence in Escherichia coli. Virulence, 2012;3(1):18\u201328.","DOI":"10.4161\/viru.3.1.18382"},{"key":"ref46","doi-asserted-by":"crossref","unstructured":"47.\tSilva V., Ribeiro J., Rocha J., Manaia C. M., Silva A., Pereira J. E., Maltez L., Capelo J. L., Igrejas G., Poeta P. High frequency of the EMRSA-15 clone (ST22-MRSA-IV) in hospital wastewater. Microorganisms, 2022;10(1):147.","DOI":"10.3390\/microorganisms10010147"},{"key":"ref47","doi-asserted-by":"crossref","unstructured":"48.\tAlsaab F., Wahdan A., Saeed E. M. A. Phenotypic detection and genotyping of Clostridium perfringens associated with enterotoxemia in sheep in the Qassim Region of Saudi Arabia. Vet. World, 2021;14(3):578\u2013584.","DOI":"10.14202\/vetworld.2021.578-584"},{"key":"ref48","doi-asserted-by":"crossref","unstructured":"49.\tElkenany R., Elsayed M., Zakaria A., Elnagar R., Salem M., Auob A., Awad A. Epidemiology and multidrug resistance of Clostridium perfringens in broiler farms and genetic diversity of multidrug resistance strains. Sci. Rep., 2025;15(1):28638.","DOI":"10.1038\/s41598-025-12519-0"},{"key":"ref49","doi-asserted-by":"crossref","unstructured":"50.\tNhung N. T., Chansiripornchai N., Carrique-Mas J. J. Antimicrobial resistance in poultry pathogens: A review. Front. Vet. Sci., 2017;4:126.","DOI":"10.3389\/fvets.2017.00126"},{"key":"ref50","doi-asserted-by":"crossref","unstructured":"51.\tKumari P., Tripathi B. M., Dong K., Eo K. Y., Lee W. S., Kimura J., Yamamoto N. The host-specific resistome in environmental feces of Eurasian otters (Lutra lutra) and leopard cats (Prionailurus bengalensis) revealed by metagenomic sequencing. One Health, 2022;14:100385.","DOI":"10.1016\/j.onehlt.2022.100385"},{"key":"ref51","doi-asserted-by":"crossref","unstructured":"52.\tLyras D., Rood J. I. Genetic organization and distribution of tetracycline resistance determinants in Clostridium perfringens. Antimicrob. Agents Chemother., 1996;40(11):2500\u20132504.","DOI":"10.1128\/AAC.40.11.2500"},{"key":"ref52","doi-asserted-by":"crossref","unstructured":"53.\tWei B., Cha S. Y., Zhang J. F., Shang K., Park H. C., Kang J., Lee K. J., Kang M., Jang H. K. Antimicrobial susceptibility and association with toxin determinants in Clostridium perfringens isolates from chickens. Microorganisms, 2020;8(11):1825.","DOI":"10.3390\/microorganisms8111825"},{"key":"ref53","doi-asserted-by":"crossref","unstructured":"54.\tFan Y. C., Wang C. L., Wang C., Chen T. C., Chou C. H., Tsai H. J. Incidence and antimicrobial susceptibility to Clostridium perfringens in premarket broilers in Taiwan. Avian Dis., 2016;60(2):444\u2013449.","DOI":"10.1637\/11315-110915-Reg"}],"container-title":["Veterinary World"],"original-title":[],"link":[{"URL":"https:\/\/veterinaryworld.org\/Vol.19\/January-2026\/29.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T17:17:27Z","timestamp":1770052647000},"score":1,"resource":{"primary":{"URL":"https:\/\/veterinaryworld.org\/Vol.19\/January-2026\/29.php"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,31]]},"references-count":54,"URL":"https:\/\/doi.org\/10.14202\/vetworld.2026.409-421","relation":{},"ISSN":["0972-8988","2231-0916"],"issn-type":[{"value":"0972-8988","type":"print"},{"value":"2231-0916","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,31]]},"article-number":"29"}}