{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:57:38Z","timestamp":1787162258518,"version":"build-2736575974"},"reference-count":48,"publisher":"Veterinary World","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. World"],"published-print":{"date-parts":[[2026,1,31]]},"abstract":"<jats:p>Background and Aim: Antimicrobial resistance (AMR) in foodborne bacteria presents a significant threat to public health, especially in countries with intensive livestock production systems. Pig farming is a major source of animal protein in Thailand and is recognized as an important reservoir of antimicrobial-resistant bacteria. Escherichia coli is commonly used as an indicator organism for monitoring AMR, including extended-spectrum \u03b2-lactamase (ESBL) production and pathogenic potential. This study aimed to assess the frequency of AMR, multidrug-resistant (MDR), ESBL determinants, and virulence genes in E. coli isolates collected from slaughterhouses and fresh markets in central Thailand. \n\nMaterials and Methods: A total of 498 archived E. coli isolates were analyzed, including 236 isolates from slaughterhouses (feces and carcasses) and 262 isolates from fresh markets (pork and cutting boards). Antimicrobial susceptibility testing was performed against 18 antimicrobial agents using the disk diffusion method. MDR was defined as resistance to three or more antimicrobial classes. ESBL production was identified through phenotypic confirmatory tests, and ESBL-producing isolates were screened for blaTEM, blaCTX-M, and blaSHV genes by multiplex polymerase chain reaction. All isolates were further examined for select virulence genes linked to major E. coli pathotypes. \n\nResults: Overall, 97.4% of E. coli isolates showed resistance to at least one antimicrobial agent, and 87.3% were classified as MDR. ESBL-producing E. coli made up 23.5% of all isolates, with a significantly higher prevalence in slaughterhouses compared to fresh markets (p &lt; 0.05). Among ESBL producers, 97.4% exhibited MDR phenotypes. Most (89.7%) of the ESBL-producing isolates carried at least one bla gene, with blaTEM being the most common, followed by blaCTX-M. Virulence genes were detected at a low frequency (3.2%), mainly involving eaeA, lt, and stp. \n\nConclusion: The high prevalence of AMR, MDR, and ESBL-producing E. coli throughout the pork production chain highlights slaughterhouses and fresh markets as key points for the spread of resistant bacteria. These findings emphasize the need for stronger antimicrobial stewardship, better hygiene practices, and ongoing AMR surveillance within the One Health approach to reduce public health risks linked to pork consumption. \n\nKeywords: antimicrobial resistance, extended-spectrum beta-lactamase, Escherichia coli, food safety, multidrug resistance, pork production chain, slaughterhouse contamination, Thailand, virulence genes.<\/jats:p>","DOI":"10.14202\/vetworld.2026.52-64","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T16:20:59Z","timestamp":1768234859000},"page":"52","update-policy":"https:\/\/doi.org\/10.14202\/vetworld.2022.policies","source":"Crossref","is-referenced-by-count":2,"title":["Antimicrobial resistance, extended-spectrum \u03b2-lactamase determinants, and virulence gene profiles of Escherichia coli along the pork production chain in central Thailand"],"prefix":"10.14202","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3291-9727","authenticated-orcid":false,"given":"Watsawan","family":"Prapasawat","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05qebwp08","id-type":"ROR","asserted-by":"publisher"}],"name":"Mahanakorn University of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8908-7875","authenticated-orcid":false,"given":"Achiraya","family":"Siriphap","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00a5mh069","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Phayao"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1190-2550","authenticated-orcid":false,"given":"Sirikarn","family":"Wiriyasirivaj","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05qebwp08","id-type":"ROR","asserted-by":"publisher"}],"name":"Mahanakorn University of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7452-6975","authenticated-orcid":false,"given":"Apiradee","family":"Intarapuk","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03b5p6e80","id-type":"ROR","asserted-by":"publisher"}],"name":"Chulabhorn Royal Academy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1482-6714","authenticated-orcid":false,"given":"Ruttana","family":"Pachanon","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02e16g702","id-type":"ROR","asserted-by":"publisher"}],"name":"Hokkaido University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9194-1062","authenticated-orcid":false,"given":"Chie","family":"Nakajima","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02e16g702","id-type":"ROR","asserted-by":"publisher"}],"name":"Hokkaido University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1313-1494","authenticated-orcid":false,"given":"Yasuhiko","family":"Suzuki","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02e16g702","id-type":"ROR","asserted-by":"publisher"}],"name":"Hokkaido University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7264-5068","authenticated-orcid":false,"given":"Orasa","family":"Suthienkul","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/002yp7f20","id-type":"ROR","asserted-by":"publisher"}],"name":"Thammasat University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5308","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"ref0","doi-asserted-by":"crossref","unstructured":"1.\tMancuso G, Midiri A, Gerace E, Biondo C. Bacterial antibiotic resistance: The most critical pathogens. Pathogens (Basel). 2021;10(10):1310.","DOI":"10.3390\/pathogens10101310"},{"key":"ref1","doi-asserted-by":"crossref","unstructured":"2.\tChonsin K, Changkwanyeun R, Siriphap A, Intarapuk A, Prapasawat W, Changkaew K, Pulsrikarn C, Isoda N, Nakajima C, Suzuki Y, Suthienkul O. Prevalence and multidrug resistance of Salmonella in swine production chain in a central province, Thailand. J Food Prot. 2021;84(12):2174\u20132184.","DOI":"10.4315\/JFP-21-003"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"3.\tLekagul A, Tangcharoensathien V, Liverani M, Mills A, Rushton J, Yeung S. Understanding antibiotic use for pig farming in Thailand: A qualitative study. Antimicrob Resist Infect Control. 2021;10(1):3.","DOI":"10.1186\/s13756-020-00865-9"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"4.\tTrongjit S, Assavacheep P, Samngamnim S, My TH, An VTT, Simjee S, Chuanchuen R. Plasmid-mediated colistin resistance and ESBL production in Escherichia coli from clinically healthy and sick pigs. Sci Rep. 2022;12(1):2466.","DOI":"10.1038\/s41598-022-06415-0"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"5.\tMa F, Xu S, Tang Z, Li Z, Zhang L. Use of antimicrobials in food animals and impact of transmission of antimicrobial resistance on humans. Biosaf Health. 2021;3(1):32\u201338.","DOI":"10.1016\/j.bsheal.2020.09.004"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"6.\tCastanheira M, Simner PJ, Bradford PA. Extended-spectrum beta-lactamases: An update on their characteristics, epidemiology and detection. JAC Antimicrob Resist. 2021;3(3):dlab092.","DOI":"10.1093\/jacamr\/dlab092"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"7.\tSongsaeng W, Am-in N, Prapasarakul N, Sirichokchatchawan W. Multidrug-resistant ESBL-producing Escherichia coli coexisting with colistin-resistance genes in pig farms, Central Thailand. Thai J Vet Med. 2024;54(1):69\u201376.","DOI":"10.56808\/2985-1130.3721"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"8.\tBarros MM, Castro J, Ara\u00fajo D, Campos AM, Oliveira R, Silva S, Outor-Monteiro D, Almeida C. Swine colibacillosis: Global epidemiologic and antimicrobial scenario. Antibiotics (Basel). 2023;12(4):682.","DOI":"10.3390\/antibiotics12040682"},{"key":"ref8","unstructured":"9.\tDo KH, Byun JW, Lee WK. Antimicrobial resistance, adhesin and toxin genes of porcine pathogenic Escherichia coli following the ban on antibiotics as the growth promoters in feed. Pak Vet J. 2021;41(4):519\u2013523."},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"10.\tYang SC, Lin CH, Aljuffali IA, Fang JY. Current pathogenic Escherichia coli foodborne outbreak cases and therapy development. Arch Microbiol. 2017;199(6):811\u2013825.","DOI":"10.1007\/s00203-017-1393-y"},{"key":"ref10","unstructured":"11.\tOffice of Agricultural Economics. Agriculture in Thailand. Bangkok: Office of Agricultural Economics; 1992."},{"key":"ref11","unstructured":"12.\tFeng P, Weagant SD. BAM Chapter 4A: Diarrheagenic Escherichia coli. In: Laboratory Methods (Food) [Internet]. Silver Spring: US Food and Drug Administration; 2020."},{"key":"ref12","unstructured":"13.\tClinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 30th informational supplement. M100. Wayne (PA): CLSI; 2020."},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"14.\tMagiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, Harbarth S, Hindler JF, Kahlmeter G, Olsson-Liljequist B, Paterson DL. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012;18(3):268\u2013281.","DOI":"10.1111\/j.1469-0691.2011.03570.x"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"15.\tYu Y, Ji S, Chen Y, Zhou W, Wei Z, Li L, Ma Y. Resistance of strains producing extended-spectrum \u03b2-lactamases and genotype distribution in China. J Infect. 2007;54(1):53\u201357.","DOI":"10.1016\/j.jinf.2006.01.014"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"16.\tRayamajhi N, Kang SG, Lee DY, Kang ML, Lee SI, Park KY, Lee HS, Yoo HS. Characterization of TEM, SHV and AmpC-type \u03b2-lactamases from cephalosporin-resistant Enterobacteriaceae isolated from swine. Int J Food Microbiol. 2008;124(2):183\u2013187.","DOI":"10.1016\/j.ijfoodmicro.2008.03.009"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"17.\tBatchelor M, Hopkins K, Threlfall EJ, Clifton-Hadley FA, Stallwood AD, Davies RH, Liebana E. blaCTX-M genes in clinical Salmonella isolates recovered from humans in England and Wales from 1992 to 2003. Antimicrob Agents Chemother. 2005;49(4):1319\u20131322.","DOI":"10.1128\/AAC.49.4.1319-1322.2005"},{"key":"ref17","unstructured":"18.\tPrapasawat W, Intarapuk A, Chompook P, Nakajima C, Suzuki Y, Suthienkul O. Antimicrobial resistance, integron, virulence gene, and multilocus sequence typing of Escherichia coli isolates from postweaning piglets with and without diarrhea. Southeast Asian J Trop Med Public Health. 2017;48(5):1042\u20131055."},{"key":"ref18","unstructured":"19.\tPachanon R. Detection of pathogenic Escherichia coli and their antimicrobial resistance in diarrheal swine [master\u2019s thesis]. Bangkok: Mahidol University; 2014."},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"20.\tFerraz MP. Antimicrobial resistance: The impact from and on society according to One Health approach. Soc. 2024;14(9):187.","DOI":"10.3390\/soc14090187"},{"key":"ref20","unstructured":"21.\tPazra DF, Iryawati D. Genetic characteristics of antimicrobial resistance in Escherichia coli isolated from farm animals, slaughterhouses, and associated environments. London: IntechOpen; 2025."},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"22.\tPrapasarakul N, Tummaruk P, Niyomtum W, Tripipat T, Serichantalergs O. Virulence genes and antimicrobial susceptibilities of hemolytic and nonhemolytic Escherichia coli isolated from post-weaning piglets in central Thailand. J Vet Med Sci. 2010;72(12):1603\u20131608.","DOI":"10.1292\/jvms.10-0124"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"23.\tVan Thong N, Thuan NK, Le Minh BT. Prevalence of antibiotic resistance genes of Escherichia coli at the pig slaughterhouses in the Mekong Delta. Vet Integr Sci. 2024;23(1):1\u201310.","DOI":"10.12982\/VIS.2025.005"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"24.\tPissetti C, Werlang GO, Kich JD, Cardoso M. Genotyping and antimicrobial resistance in Escherichia coli from pig carcasses. Pesq Vet Bras. 2017;37:1253\u20131260.","DOI":"10.1590\/s0100-736x2017001100010"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"25.\tKidsley AK, Abraham S, Bell JM, O\u2019Dea M, Laird TJ, Jordan D, Mitchell P, McDevitt CA, Trott DJ. Antimicrobial susceptibility of Escherichia coli and Salmonella spp. isolates from healthy pigs in Australia: Results of a pilot national survey. Front Microbiol. 2018;9:1207.","DOI":"10.3389\/fmicb.2018.01207"},{"key":"ref25","unstructured":"26.\tEuropean Medicines Agency. European surveillance of veterinary antimicrobial consumption, 2022. Sales of veterinary antimicrobial agents in 31 European countries in 2022. Amsterdam: EMA; 2023."},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"27.\tGon\u00e7alves C, Silveira L, Rodrigues J, Furtado R, Ramos S, Nunes A, Pista \u00c2. Phenotypic and genotypic characterization of Escherichia coli and Salmonella spp. isolates from pigs at slaughterhouse and from commercial pork meat in Portugal. Antibiotics (Basel). 2024;13(10):957.","DOI":"10.3390\/antibiotics13100957"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"28.\tTrongjit S, Angkittitrakul S, Chuanchuen R. Occurrence and molecular characteristics of antimicrobial resistance of Escherichia coli from broilers, pigs and meat products in Thailand and Cambodia provinces. J Microbiol Immunol. 2016;60(9):575\u2013585.","DOI":"10.1111\/1348-0421.12407"},{"key":"ref28","doi-asserted-by":"crossref","unstructured":"29.\tSheikh M, Gholipour S, Ghodsi S, Nikaeen M. Co-selection of antibiotic and disinfectant resistance in environmental bacteria: Health implications and mitigation strategies. Environ Res. 2025;267:120708.","DOI":"10.1016\/j.envres.2024.120708"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"30.\tHossain AZ, Chowdhury AMA. Understanding the evolution and transmission dynamics of antibiotic resistance genes: A comprehensive review. J Basic Microbiol. 2024;64(10):e2400259.","DOI":"10.1002\/jobm.202400259"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"31.\tTreilles M, Ch\u00e2tre P, Drapeau A, Madec JY, Haenni M. Spread of the mcr-1 colistin-resistance gene in Escherichia coli through plasmid transmission and chromosomal transposition in French goats. Front Microbiol. 2023;13:1023403.","DOI":"10.3389\/fmicb.2022.1023403"},{"key":"ref31","doi-asserted-by":"crossref","unstructured":"32.\tBoonyasiri A, Tangkoskul T, Seenama C, Saiyarin J, Tiengrim S, Thamlikitkul V. Prevalence of antibiotic resistant bacteria in healthy adults, foods, food animals, and the environment in selected areas in Thailand. Pathog Glob Health. 2014;108(5):235\u2013245.","DOI":"10.1179\/2047773214Y.0000000148"},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"33.\tSornsenee P, Chimplee S, Arbubaker A, Kongchai S, Madimong H, Romyasamit C. Occurrence, antimicrobial resistance profile, and characterization of extended-spectrum \u03b2-lactamase-producing Escherichia coli isolates from minced meat at local markets in Thailand. Foodborne Pathog Dis. 2022;19(3):232\u2013240.","DOI":"10.1089\/fpd.2021.0059"},{"key":"ref33","doi-asserted-by":"crossref","unstructured":"34.\tGuo S, Aung KT, Leekitcharoenphon P, Tay MY, Seow KL, Zhong Y, Ng LC, Aarestrup FM, Schlundt J. Prevalence and genomic analysis of ESBL-producing Escherichia coli in retail raw meats in Singapore. J Antimicrob Chemother. 2021;76(3):601\u2013605.","DOI":"10.1093\/jac\/dkaa461"},{"key":"ref34","doi-asserted-by":"crossref","unstructured":"35.\tLe QP, Ueda S, Nguyen TNH, Dao TVK, Van Hoang TA, Tran TTN, Hirai I, Nakayama T, Kawahara R, Do TH, Vien QM. Characteristics of extended-spectrum \u03b2-lactamase\u2013producing Escherichia coli in retail meats and shrimp at a local market in Vietnam. Foodborne Pathog Dis. 2015;12(8):719\u2013725.","DOI":"10.1089\/fpd.2015.1954"},{"key":"ref35","doi-asserted-by":"crossref","unstructured":"36.\tKim YJ, Moon JS, Oh DH, Chon JW, Song BR, Lim JS, Heo EJ, Park HJ, Wee SH, Sung K. Genotypic characterization of ESBL-producing Escherichia coli from imported meat in South Korea. Food Res Int. 2018;107:158\u2013164.","DOI":"10.1016\/j.foodres.2017.12.023"},{"key":"ref36","doi-asserted-by":"crossref","unstructured":"37.\tMatakone M, Founou RC, Founou LL, Dimani BD, Koudoum PL, Fonkoua MC, Boum-Ii Y, Gonsu H, Noubom M. Multidrug-resistant and extended-spectrum \u03b2-lactamase-producing Escherichia coli isolated from slaughtered pigs and slaughterhouse workers in Yaound\u00e9, Cameroon. One Health. 2024;19:100885.","DOI":"10.1016\/j.onehlt.2024.100885"},{"key":"ref37","doi-asserted-by":"crossref","unstructured":"38.\tSaenkankam I, Apiwatsiri P, Supimon N, Niyomtham W, Hampson DJ, Prapasarakul N. Tracking extended-spectrum \u03b2-lactamase-producing and colistin-resistant Escherichia coli in pig abattoirs: Impacts on food safety. Foodborne Pathog Dis. 2025;10(1089):40229124.","DOI":"10.1089\/fpd.2024.0178"},{"key":"ref38","doi-asserted-by":"crossref","unstructured":"39.\tCoelho MMS, Davanzo EFA, Dos Santos RL, Castro VHDL, da Costa HMB, Dallago BSL, Perecmanis S, Santana AP. Escherichia coli and Enterobacteriaceae counts, virulence gene profile, antimicrobial resistance, and biofilm formation capacity during pig slaughter stages. Life. 2024;14(10):1261.","DOI":"10.3390\/life14101261"},{"key":"ref39","doi-asserted-by":"crossref","unstructured":"40.\tHide M, Meng S, Cheng S, Ba\u00f1uls AL, Ky S, Yay C, Laurent D, Delvallez G. Colistin resistance in ESBL- and carbapenemase-producing Escherichia coli and Klebsiella pneumoniae clinical isolates in Cambodia. J Glob Antimicrob Resist. 2024;38:236\u2013244.","DOI":"10.1016\/j.jgar.2024.06.017"},{"key":"ref40","doi-asserted-by":"crossref","unstructured":"41.\tHussain HI, Aqib AI, Seleem MN, Shabbir MA, Hao H, Iqbal Z, Kulyar MFEA, Zaheer T, Li K. Genetic basis of molecular mechanisms in \u03b2-lactam resistant gram-negative bacteria. Microb Pathog. 2021;158:105040.","DOI":"10.1016\/j.micpath.2021.105040"},{"key":"ref41","doi-asserted-by":"crossref","unstructured":"42.\tLekagul A, Tangcharoensathien V, Mills A, Rushton J, Yeung S. How antibiotics are used in pig farming: A mixed-methods study of pig farmers, feed mills and veterinarians in Thailand. BMJ Glob Health. 2020;5(2):e001918.","DOI":"10.1136\/bmjgh-2019-001918"},{"key":"ref42","doi-asserted-by":"crossref","unstructured":"43.\tLugsomya K, Chatsuwan T, Niyomtham W, Tummaruk P, Hampson DJ, Prapasarakul N. Routine prophylactic antimicrobial use is associated with increased phenotypic and genotypic resistance in commensal Escherichia coli isolates recovered from healthy fattening pigs on farms in Thailand. Microb Drug Resist. 2018;24(2):213\u2013223.","DOI":"10.1089\/mdr.2017.0042"},{"key":"ref43","doi-asserted-by":"crossref","unstructured":"44.\tLay KK, Torio HE, Bitrus AA, Mala W, Sinwat N, Chuanchuen R. Multidrug-resistant Escherichia coli harboring extended-spectrum \u03b2-lactamase-encoding genes isolated from clinically healthy pigs. Thai J Vet Med. 2021;51(2):303\u2013310.","DOI":"10.56808\/2985-1130.3122"},{"key":"ref44","doi-asserted-by":"crossref","unstructured":"45.\tValentin L, Sharp H, Hille K, Seibt U, Fischer J, Pfeifer Y, Michael GB, Nickel S, Schmiedel J, Falgenhauer L, Friese A. Subgrouping of ESBL-producing Escherichia coli from animal and human sources: An approach to quantify the distribution of ESBL types between different reservoirs. Int J Med Microbiol. 2014;304(7):805\u2013816.","DOI":"10.1016\/j.ijmm.2014.07.015"},{"key":"ref45","doi-asserted-by":"crossref","unstructured":"46.\tChishimba K, Hang\u2019Ombe BM, Muzandu K, Mshana SE, Matee MI, Nakajima C, Suzuki Y. Detection of extended-spectrum beta-lactamase-producing Escherichia coli in market-ready chickens in Zambia. Int J Microbiol. 2016;2016:5275724.","DOI":"10.1155\/2016\/5275724"},{"key":"ref46","doi-asserted-by":"crossref","unstructured":"47.\tHusna A, Rahman MM, Badruzzaman ATM, Sikder MH, Islam MR, Rahman MT, Alam J, Ashour HM. Extended-spectrum \u03b2-lactamases: Challenges and opportunities. Biomedicines. 2023;11(11):2937.","DOI":"10.3390\/biomedicines11112937"},{"key":"ref47","unstructured":"48.\tRay B, Bhunia A. Escherichia coli gastroenteritis. In: Fundamental food microbiology. 4th ed. New York: CRC Press; 2008. p. 323\u2013326."}],"container-title":["Veterinary World"],"original-title":[],"link":[{"URL":"https:\/\/veterinaryworld.org\/Vol.19\/January-2026\/5.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T16:21:44Z","timestamp":1768234904000},"score":1,"resource":{"primary":{"URL":"https:\/\/veterinaryworld.org\/Vol.19\/January-2026\/5.php"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,8]]},"references-count":48,"URL":"https:\/\/doi.org\/10.14202\/vetworld.2026.52-64","relation":{},"ISSN":["0972-8988","2231-0916"],"issn-type":[{"value":"0972-8988","type":"print"},{"value":"2231-0916","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,8]]},"article-number":"5"}}