{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T18:42:42Z","timestamp":1740163362628,"version":"3.37.3"},"reference-count":31,"publisher":"Veterinary World","license":[{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"vor","delay-in-days":23,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"tdm","delay-in-days":23,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/00772\/2020"],"award-info":[{"award-number":["UIDB\/00772\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet World"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:p>Background and Aim: Anemia, a clinical condition characterized by reduced erythrocytes, is often observed in cats. Regeneration indicates that the bone marrow can respond appropriately to anemia. The absolute reticulocyte count is the reference for differentiating regenerative and non-regenerative anemia, while red blood cell (RBC) indices and morphology provide supplementary information. This study aimed to identify anemia types and establish the most reliable RBC indices and morphology methods in agreement with the reference method. \n\nMaterials and Methods: One hundred forty-five cases of cat anemia were prospectively classified using two methods: RBC indices and RBC morphology, and subsequently compared with the absolute reticulocyte count. \n\nResults: Based on RBC indices assessment, 27 cases (19%) exhibited regenerative anemia. Based on RBC morphology, 29 (20%) cases were identified as having regenerative anemia. Using the reticulocyte absolute count as a reference method, 34 (23.4%) cases of regenerative anemia were identified. The findings indicated that RBC indices and RBC morphology did not align in evaluating medullary regeneration and that there is a good degree of agreement between RBC morphology assessment and the reticulocyte absolute count in identifying regenerative anemias. \n\nConclusion: Blood smear analysis of RBC morphology was more dependable for classifying regenerative anemia than RBC indices. Further studies should be conducted with a larger number of animals and that allow the identification of the cause of anemia and the monitoring of the animal. \n\nKeywords: anemia, cats, red blood cell indices, red blood cell morphology, reticulocyte.<\/jats:p>","DOI":"10.14202\/vetworld.2024.1591-1595","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T17:23:29Z","timestamp":1721841809000},"page":"1591-1595","update-policy":"https:\/\/doi.org\/10.14202\/vetworld.2022.policies","source":"Crossref","is-referenced-by-count":0,"title":["Regenerative anemia identification in cats: Red blood cell indices or morphology, what to use?"],"prefix":"10.14202","author":[{"given":"Ana Catarina","family":"da Silva Teixeira Fernandes","sequence":"first","affiliation":[{"name":"Department of Clinical Biology, School of Life and Environmental Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, Quinta de Prados, Vila Real, Portugal."}]},{"given":"Daniela Maria Carneiro","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Health and Food Safety (Segalab), Argivai, P\u00f3voa de Varzim, Portugal."}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6130-8381","authenticated-orcid":false,"given":"Felisbina Lu\u00edsa","family":"Queiroga","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, Quinta de Prados, Vila Real, Portugal; Animal and Veterinary Research Centre, University of Tr\u00e1s-os-Montes and Alto Douro, Vila Real, Portugal; Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), Quinta de Prados, Vila Real, Portugal."}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7114-6918","authenticated-orcid":false,"given":"Ana C.","family":"Silvestre-Ferreira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, Quinta de Prados, Vila Real, Portugal; Animal and Veterinary Research Centre, University of Tr\u00e1s-os-Montes and Alto Douro, Vila Real, Portugal; Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), Quinta de Prados, Vila Real, Portugal."}]}],"member":"5308","published-online":{"date-parts":[[2024,7,24]]},"reference":[{"key":"ref0","unstructured":"Fleischman, W. (2012) Anemia: Determining the cause. Compend. Contin. Educ. Vet., 34(6): E1."},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Chervier, C., Cador\u00e9, J.L., Rodriguez-Pi\u00f1eiro, M.I., Deputte, B.L. and Chabanne, L. (2012) Causes of anaemia other than acute blood loss and their clinical significance in dogs. J. Small Anim. Pract., 53(4): 223\u2013227.","DOI":"10.1111\/j.1748-5827.2011.01191.x"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Lynch, A.M., Respess, M., Boll, A.E., Bozych, M., McMichael, M., Fletcher, D.J., De Laforcade, A.M. and Rozanski, E.A. (2016) Hospital-acquired anemia in critically Ill dogs and cats: A multi-institutional study. J. Vet. Intern. Med., 30(1): 141\u2013146.","DOI":"10.1111\/jvim.13650"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Tasker, S. (2012) Diagnostic approach to anaemia in cats. In Pract., 34(7): 370\u2013381.","DOI":"10.1136\/inp.e4889"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Paltrinieri, S., Rossi, G., Manca, M., Scarpa, P., Vitiello, T. and Giordano, A. (2016) Sensitivity and specificity of manual and automated measurements of reticulocyte parameters for classification of anemia in dogs: 174 cases (1993\u20132013). J. Am. Vet. Med. Assoc., 249(7): 776\u2013786.","DOI":"10.2460\/javma.249.7.776"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Yadav, S.N., Ahmed, N., Nath, A.J. and Boro, P.K. (2022) Erythrocyte parameters as a diagnostic tool in canine medicine: A review. Agric. Rev., 43(3): 288\u2013295.","DOI":"10.18805\/ag.r-2112"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Hodges, J. and Christopher, M.M. (2011) Diagnostic accuracy of using erythrocyte indices and polychromasia to identify regenerative anemia in dogs. J. Am. Vet. Med. Assoc., 238(11): 1452\u20131458.","DOI":"10.2460\/javma.238.11.1452"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Weiss, D.J. (1984) Uniform evaluation and semiquantitative reporting of hematologic data in veterinary laboratories. Vet. Clin. Pathol., 13(1): 27\u201331.","DOI":"10.1111\/j.1939-165x.1984.tb00836.x"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Hunt, A. and Jugan, M.C. (2021) Anemia, iron deficiency, and cobalamin deficiency in cats with chronic gastrointestinal disease. J. Vet. Intern. Med., 35(1): 172\u2013178.","DOI":"10.1111\/jvim.15962"},{"key":"ref9","unstructured":"Tasker, S. (2006) The Differential Diagnosis of Feline Anaemia. WSAVA World Congress. Available from: https:\/\/www.vin.com\/apputil\/content\/defaultadv1.aspx?id=3859015&pid=11223.  Retrieved on 15-05-2023."},{"key":"ref10","unstructured":"Tvedten, H. (2022) Classification and laboratory evaluation of anemia. In: Brooks, M.B., Harr, K.E., Seelig, D.M., Wardrop, K.J. and Weiss, D.J., editors. Schalm\u2019s Veterinary Hematology. 7th ed. John Wiley and Sons, Inc., Hoboken."},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Cohen, J. (1968) Weighted kappa: Nominal scale agreement with provision for scaled disagreement or partial credit. Psychol. Bull., 70(4): 213\u2013220.","DOI":"10.1037\/h0026256"},{"key":"ref12","unstructured":"Altman, D. (1991) Practical Statistics for Medical Research. Chapman and Hall\/CRC Texts in Statistical Science, London."},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Piva, E., Brugnara, C., Chiandetti, L. and Plebani, M. (2010) Automated reticulocyte counting: State of the art and clinical applications in the evaluation of erythropoiesis. Clin. Chem. Lab. Med., 48(10): 1369\u20131380.","DOI":"10.1515\/cclm.2010.292"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"Papasouliotis, K. and Murphy, K. (2021) Pictorial guide to canine and feline blood smears. Part 1: Normal cells. In Pract., 47(6): 311\u2013317.","DOI":"10.1002\/inpr.79"},{"key":"ref15","unstructured":"Adewoyin, A.S. and Nwogoh, B. (2014) Peripheral blood film - A review. Ann. Ib. Postgrad. Med., 12(2): 71\u201379."},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Winzelberg, O.S. and Hohenhaus, A.E. (2019) Feline non-regenerative anemia: Diagnostic and treatment recommendations. J. Feline Med. Surg., 21(7): 615\u2013631.","DOI":"10.1177\/1098612x19856178"},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Korman, R.M., Hetzel, N., Knowles, T.G., Harvey, A.M. and Tasker, S. (2013) A retrospective study of 180 anaemic cats: Features, aetiologies and survival data. J. Feline Med. Surg., 15(2): 81\u201390.","DOI":"10.1177\/1098612x12461008"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Furman, E., Leidinger, E., Hooijberg, E.H., Bauer, N., Beddies, G. and Moritz, A. (2014) A retrospective study of 1,098 blood samples with anemia from adult cats: Frequency, classification, and association with serum creatinine concentration. J. Vet. Intern. Med., 28(5): 1391\u20131397.","DOI":"10.1111\/jvim.12422"},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Samly, S.S.M., Mohanm, R.C.P., Sivarankaran, M. and Sadasivan, S. (2019) Comparison of hematological parameters with peripheral smear in anemia. Int. J. Clin. Diagn. Pathol., 2(1): 92\u201395.","DOI":"10.33545\/pathol.2019.v2.i1b.17"},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Keiner, M., Fuchs, J., Bauer, N. and Moritz, A. (2020) Evaluation of reticulocyte hemoglobin content (RETIC-HGB) for the diagnosis of iron-limited erythropoiesis in cats. Vet. Clin. Pathol., 49(4): 557\u2013566.","DOI":"10.1111\/vcp.12925"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"DeNicola, D.B. (2020) Interpreting Routine Feline Haematology: What do Red Cell Parameters Really Mean? IDEXX, International Cat Care. Available from: https:\/\/icatcare.org\/event\/isfm-vet-and-nurse-webinar-free-access-what-do-red-cell-parameters-really-mean. Retrieved on 04-01-2024.","DOI":"10.22233\/9781910443491.idexxwebinar230124"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"Tvedten, H. and Moritz, A. (2022) Reticulocyte and heinz body staining and enumeration. In: Brooks, M.B., Harr, E.K., Seelig, D.M., Wardrop, K.J. and Weiss, D.J., editors. Schalm\u2019s Veterinary Hematology. 7th ed. Wiley-Blackwell, Hoboken.","DOI":"10.1002\/9781119500537.ch23"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Paltrinieri, S., Fossati, M. and Menaballi, V. (2018) Diagnostic performances of manual and automated reticulocyte parameters in anaemic cats. J. Feline Med. Surg., 20(2): 122\u2013127.","DOI":"10.1177\/1098612x17699067"},{"key":"ref24","unstructured":"Belo, A.F.C. (2015) Avalia\u00e7\u00e3o dos \u00cdndices Eritrocit\u00e1rios MCV, MCHC e RDW e a sua Compara\u00e7\u00e3o com a Resposta Reticulocit\u00e1ria em C\u00e3es e Gatos An\u00e9micos. Masters Dissertation, Lus\u00f3fona University of Humanities and Technologies, Lisbon, Portugal."},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"Chang L., Yore, K., DeNicola, D.B. and Donald, S. (2023) Performance of Red Blood Cell Indices for the Detection of Reticulocytosis in Anemic and Non-Anemic Dogs in Taiwan. Available from: https:\/\/www.ssrn.4559205. Retrieved on 04-01-2024.","DOI":"10.2139\/ssrn.4559205"},{"key":"ref26","doi-asserted-by":"publisher","unstructured":"Betting, A., Schweighauser, A. and Francey, T. (2022) Diagnostic value of reticulocyte indices for the assessment of the iron status of cats with chronic kidney disease. J. Vet. Intern. Med., 36(2): 619\u2013628.","DOI":"10.1111\/jvim.16367"},{"key":"ref27","doi-asserted-by":"publisher","unstructured":"Banfi, G., Salvagno, G.L. and Lippi, G. (2007) The role of ethylenediaminetetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes. Clin. Chem. Lab. Med., 45(5): 565\u2013576.","DOI":"10.1515\/cclm.2007.110"},{"key":"ref28","doi-asserted-by":"publisher","unstructured":"Narasimha, A., Kumar, H. and Prasad, C.S.B.R. (2008) Anticoagulant induced artifacts in peripheral blood smears. Indian J. Hematol. Blood Transfs., 24(2): 43\u201348.","DOI":"10.1007\/s12288-008-0027-6"},{"key":"ref29","doi-asserted-by":"publisher","unstructured":"Santoro, P. (2018) Manual methods vs automated hematology analyzers in veterinary hematology. Vet. Clin. Pathol., 47(2): 178.","DOI":"10.1111\/vcp.12611"},{"key":"ref30","doi-asserted-by":"publisher","unstructured":"Harvey, J.W. (2017) The feline blood film: 1. Techniques and erythrocyte morphology. J. Feline Med. 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