{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T14:51:45Z","timestamp":1786546305978,"version":"build-2736575974"},"reference-count":36,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Transl Gastroenterol","Clinical and Translational Gastroenterology"],"abstract":"<jats:sec>\n                    <jats:title>INTRODUCTION:<\/jats:title>\n                    <jats:p>In patients with ulcerative colitis (UC), dysplasia develops in 10%\u201320% of cases. The persistence of low-grade dysplasia (LGD) in UC in 2 consecutive observations is still an indication for restorative proctocolectomy. Our hypothesis is that in the case of weak cytotoxic activation, dysplasia persists. We aimed to identify possible immunological markers of LGD presence and persistence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS:<\/jats:title>\n                    <jats:p>We prospectively enrolled 112 UC patients who underwent screening colonoscopy (T0) who had biopsies taken from their sigmoid colon. Ninety of them had at least a second colonoscopy (T1) with biopsies taken in the sigmoid colon and 8 patients had dysplasia in both examinations suggesting a persistence of LGD in their colon. Immunohistochemistry and real time polymerase chain reaction for CD4, CD69, CD107, and CD8\u03b2 messenger RNA (mRNA) expression and flow cytometry for epithelial cells expressing CD80 or HLA avidin-biotin complex were performed. Non-parametric statistics, receiver operating characteristic curves analysis, and logistic multiple regression analysis were used.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>\n                      Thirteen patients had LGD diagnosed at T0. The mucosal mRNA expression of CD4, CD69, and CD8\u03b2 was significantly lower than in patients without dysplasia (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.033,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.046 and\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.007, respectively). A second colonoscopy was performed in 90 patients after a median follow-up of 17 (12\u201325) months and 14 of the patients were diagnosed with LGD. In these patients, CD8\u03b2 mRNA expression at T0 was significantly lower in patients without dysplasia (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.004). A multivariate survival analysis in a model including CD8\u03b2 mRNA levels and age &gt;50 demonstrated that both items were independent predictors of dysplasia at follow-up (hazard ratio [HR] = 0.47 [95% confidence interval [CI]: 0.26\u20130.86],\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.014, and HR = 13.32 [95% CI: 1.72\u2013102.92],\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.013).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>DISCUSSION:<\/jats:title>\n                    <jats:p>These data suggest a low cytotoxic T cell activation in the colonic mucosa of UC patients who do not manage to clear dysplasia. Thus, low level of CD8\u03b2 mRNA expression in non-dysplastic colonic mucosa might be considered in future studies about the decision making of management of LGD in UC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.14309\/ctg.0000000000000061","type":"journal-article","created":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T18:03:59Z","timestamp":1563991439000},"page":"e00061","source":"Crossref","is-referenced-by-count":8,"title":["Weak Cytotoxic T Cells Activation Predicts Low-Grade Dysplasia Persistence in Ulcerative Colitis"],"prefix":"10.14309","volume":"10","author":[{"given":"Andromachi","family":"Kotsafti","sequence":"first","affiliation":[{"name":"Laboratory of Advanced Translational Research, Veneto Institute of Oncology IOV - IRCCS, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"D'Inc\u00e0","sequence":"additional","affiliation":[{"name":"Gastroenterology Unit, Azienda Ospedaliera di Padova, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melania","family":"Scarpa","sequence":"additional","affiliation":[{"name":"Laboratory of Advanced Translational Research, Veneto Institute of Oncology IOV - IRCCS, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Fassan","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Padova, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imerio","family":"Angriman","sequence":"additional","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology, University of Padova, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Mescoli","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Padova, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicol\u00f2","family":"Bortoli","sequence":"additional","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology, University of Padova, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Brun","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of Padova, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romeo","family":"Bardini","sequence":"additional","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology, University of Padova, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Rugge","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Padova, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edoardo","family":"Savarino","sequence":"additional","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology, University of Padova, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana","family":"Zingone","sequence":"additional","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology, University of Padova, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo","family":"Castoro","sequence":"additional","affiliation":[{"name":"Upper GI Surgery Unit, Istituto Humanitas, Rozzano, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignazio","family":"Castagliuolo","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of Padova, Padova, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Scarpa","sequence":"additional","affiliation":[{"name":"General Surgery Unit, Azienda Ospedaliera di Padova, Padova, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2019,7,23]]},"reference":[{"key":"R1-20230814","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1016\/0016-5085(92)91163-X","article-title":"Colorectal cancer risk and mortality in patients with ulcerative colitis","volume":"103","author":"Langholz","year":"1992","journal-title":"Gastroenterology"},{"key":"R2-20230814","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1136\/gut.48.4.526","article-title":"The risk of colorectal cancer in ulcerative colitis: A meta-analysis","volume":"48","author":"Eaden","year":"2001","journal-title":"Gut"},{"issue":"Suppl 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