{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T21:52:56Z","timestamp":1770414776237,"version":"3.49.0"},"reference-count":30,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2014,9,26]],"date-time":"2014-09-26T00:00:00Z","timestamp":1411689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecules and Cells"],"published-print":{"date-parts":[[2014,10]]},"DOI":"10.14348\/molcells.2014.0168","type":"journal-article","created":{"date-parts":[[2014,9,25]],"date-time":"2014-09-25T21:15:47Z","timestamp":1411679747000},"page":"727-733","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":30,"title":["Metabolic Engineering of Rational Screened Saccharopolyspora spinosa for the Enhancement of Spinosyns A and D Production"],"prefix":"10.1016","volume":"37","author":[{"given":"Amit Kumar","family":"Jha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anaya Raj","family":"Pokhrel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit Kumar","family":"Chaudhary","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong-Whan","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan Je","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Kyung","family":"Sohng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.14348\/molcells.2014.0168_bib1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/0378-1119(92)90627-2","article-title":"Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp","volume":"116","author":"Bierman","year":"1992","journal-title":"Gene"},{"key":"10.14348\/molcells.2014.0168_bib2","first-page":"968518","article-title":"An Insight into the \u201c-Omics\u201d based engineering of Streptomycetes for secondary metabolite overproduction","author":"Chaudhary","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"10.14348\/molcells.2014.0168_bib3","first-page":"133","article-title":"Naturally derived materials as products and leads for insect control: the spinosyns","volume":"2","author":"Crouse","year":"1998","journal-title":"Rev. Tox."},{"key":"10.14348\/molcells.2014.0168_bib4","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1007\/s10295-008-0431-9","article-title":"SpnH from Saccharopolyspora spinosa encodes a rhamnosyl 4-O-methyltransferase for biosynthesis of the insecticidal macrolide, spinosyn","volume":"35","author":"Huang","year":"2008","journal-title":"A. J. Ind. Microbiol. Biotechnol"},{"key":"10.14348\/molcells.2014.0168_bib5","doi-asserted-by":"crossref","first-page":"52","DOI":"10.4014\/jmb.1308.08063","article-title":"Enhancement of herboxidiene production in Streptomyces chromofuscus ATCC 49982","volume":"24","author":"Jha","year":"2014","journal-title":"J. Microbiol. Biotechnol."},{"key":"10.14348\/molcells.2014.0168_bib6","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1631\/jzus.2006.AS0366","article-title":"Improvement of spinosad producing Saccharopolyspora spinosa by rational screening","volume":"7","author":"Jin","year":"2006","journal-title":"J. Zhejiang. University SCIENCE A"},{"key":"10.14348\/molcells.2014.0168_bib7","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1007\/s12010-008-8500-0","article-title":"Enhanced production of spinosad in Saccharopolyspora spinosa by genome shuffling","volume":"159","author":"Jin","year":"2009","journal-title":"Appl. Biochem. Biotechnol"},{"key":"10.14348\/molcells.2014.0168_bib8","series-title":"Practical Streptomyces genetics","article-title":"Practical Streptomyces genetics","author":"Kieser","year":"2000"},{"key":"10.14348\/molcells.2014.0168_bib9","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1128\/JB.185.2.592-600.2003","article-title":"Accumulation of S-adenosyl-L-methionine enhances production of actinorhodin but inhibits sporulation in Streptomyces lividans TK23","volume":"185","author":"Kim","year":"2003","journal-title":"J. Bacteriol"},{"key":"10.14348\/molcells.2014.0168_bib10","doi-asserted-by":"crossref","first-page":"2901","DOI":"10.1021\/ja910223x","article-title":"The biosynthesis of spinosyn in Saccharopolyspora spinosa: synthesis of permethylated rhamnose and characterization of the functions of SpnH, SpnI, and SpnK","volume":"132","author":"Kim","year":"2010","journal-title":"J. Am. Chem. Soc"},{"key":"10.14348\/molcells.2014.0168_bib11","series-title":"Discovery, isolation and structure elucidation of a family of structurally unique, fermentation-derived tetracyclic macrolides","first-page":"214","article-title":"Discovery, isolation and structure elucidation of a family of structurally unique, fermentation-derived tetracyclic macrolides","author":"Kirst","year":"1992"},{"key":"10.14348\/molcells.2014.0168_bib12","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1146\/annurev.bioeng.1.1.535","article-title":"Metabolic engineering","volume":"1","author":"Koffas","year":"1999","journal-title":"Annu. Rev. Biomed. Eng"},{"key":"10.14348\/molcells.2014.0168_bib13","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1046\/j.1365-2958.2002.02840.x","article-title":"afsS is a target of AfsR, a transcriptional factor with ATPase activity that globally controls secondary metabolism in Streptomyces coelicolor A3(2)","volume":"43","author":"Lee","year":"2002","journal-title":"Mol. Microbiol"},{"key":"10.14348\/molcells.2014.0168_bib14","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s10059-013-0127-5","article-title":"Emerging tools for synthetic genome design","volume":"35","author":"Lee","year":"2013","journal-title":"Mol. Cells"},{"key":"10.14348\/molcells.2014.0168_bib15","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1007\/s12010-008-8281-5","article-title":"Improvement of Saccharopolyspora spinosa and the kinetic analysis for spinosad production","volume":"152","author":"Liang","year":"2009","journal-title":"Appl. Biochem. Biotechnol"},{"key":"10.14348\/molcells.2014.0168_bib16","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1477-5956-9-40","article-title":"Comparative proteomic analysis of saccharopolyspora spinosa SP06081 and PR2 strains reveals the differentially expressed proteins correlated with the increase of spinosad yield","volume":"9","author":"Luo","year":"2011","journal-title":"Proteome Sci"},{"key":"10.14348\/molcells.2014.0168_bib17","doi-asserted-by":"crossref","first-page":"5632","DOI":"10.1128\/JB.183.19.5632-5638.2001","article-title":"Rhamnose bio-synthesis pathway supplies precursors for primary and secondary metabolism in Saccharopolyspora spinosa","volume":"183","author":"Madduri","year":"2001","journal-title":"J. Bacteriol"},{"key":"10.14348\/molcells.2014.0168_bib18","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1038\/sj.jim.7000180","article-title":"Genes for the biosynthesis of spinosyns: applications for yield improvement in Saccharopolyspora spinosa","volume":"27","author":"Madduri","year":"2001","journal-title":"J. Ind. Microbiol. Biotechnol"},{"key":"10.14348\/molcells.2014.0168_bib19","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/s002530000511","article-title":"Metabolic engineering","volume":"55","author":"Nielsen","year":"2001","journal-title":"Appl. Microbiol. Botechnol"},{"key":"10.14348\/molcells.2014.0168_bib20","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1128\/JB.185.2.601-609.2003","article-title":"Enhanced expression of S-adenosylmethionine synthetase causes overproduction of actinorhodin in Streptomyces coelicolor A3 (2)","volume":"185","author":"Okamoto","year":"2003","journal-title":"J. Bacteriol"},{"key":"10.14348\/molcells.2014.0168_bib21","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1007\/s10529-010-0481-8","article-title":"Improvement of spinosad production by over-expression of gtt and gdh controlled by promoter ermE* in Saccharopolyspora spinosa SIPI-A2090","volume":"33","author":"Pan","year":"2011","journal-title":"Biotechnol. Lett"},{"key":"10.14348\/molcells.2014.0168_bib22","series-title":"Molecular cloning: a laboratory manual","article-title":"Molecular cloning: a laboratory manual","author":"Sambrook","year":"2001"},{"key":"10.14348\/molcells.2014.0168_bib23","series-title":"Methods in biotechnology, biopesticides: use and delivery","first-page":"171","article-title":"Methods in biotechnology, biopesticides: use and delivery","author":"Sparks","year":"1998"},{"key":"10.14348\/molcells.2014.0168_bib24","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.febslet.2004.04.033","article-title":"Neocarzinostatin naphthoate synthase: an unique iterative type I PKS from neocarzinostatin producer Streptomyces carzinostaticus","volume":"566","author":"Sthapit","year":"2004","journal-title":"FEBS Lett"},{"key":"10.14348\/molcells.2014.0168_bib25","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/j.1574-6968.2011.02405.x","article-title":"Duplication of partial spinosyn biosynthetic gene cluster in Saccharopolyspora spinosa enhances spinosyn production","volume":"325","author":"Tang","year":"2011","journal-title":"FEMS Microbiol. Lett"},{"key":"10.14348\/molcells.2014.0168_bib26","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1023\/A:1010289901631","article-title":"A cluster of genes for the biosynthesis of spinosyns, novel macrolide insect control agents produced by Saccharopolyspora spinosa","volume":"78","author":"Waldron","year":"2001","journal-title":"Antonie. Van. Leeuwenhoek."},{"key":"10.14348\/molcells.2014.0168_bib27","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/S1074-5521(01)00029-1","article-title":"Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa","volume":"8","author":"Waldron","year":"2001","journal-title":"Chem. Biol"},{"key":"10.14348\/molcells.2014.0168_bib28","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s00253-007-1172-9","article-title":"Improving heterologous polyketide production in Escherichia coli by overexpression of an S-adenosylmethionine synthetase gene","volume":"77","author":"Wang","year":"2007","journal-title":"Appl. Microbiol. Biotechnol"},{"key":"10.14348\/molcells.2014.0168_bib29","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.bej.2013.01.007","article-title":"Stepwise increase of spinosad production in Saccharopolyspora spinosa by metabolic engineering","volume":"72","author":"Xue","year":"2013","journal-title":"Biochem. Eng. J"},{"key":"10.14348\/molcells.2014.0168_bib30","first-page":"927","article-title":"S-Adenosylmethionine (SAM) regulates antibiotic biosynthesis in Streptomyces spp. in a mode independent of its role as a methyl donor","volume":"16","author":"Zhao","year":"2006","journal-title":"J. Microbiol. Biotechnol"}],"container-title":["Molecules and Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1016847823053670?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1016847823053670?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/ocean.kisti.re.kr\/downfile\/crosscheck\/ksmcb\/JAKO201433150756152.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T17:49:34Z","timestamp":1760896174000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1016847823053670"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10]]},"references-count":30,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["S1016847823053670"],"URL":"https:\/\/doi.org\/10.14348\/molcells.2014.0168","relation":{},"ISSN":["1016-8478"],"issn-type":[{"value":"1016-8478","type":"print"}],"subject":[],"published":{"date-parts":[[2014,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Metabolic Engineering of Rational Screened Saccharopolyspora spinosa for the Enhancement of Spinosyns A and D Production","name":"articletitle","label":"Article Title"},{"value":"Molecules and Cells","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.14348\/molcells.2014.0168","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 THE AUTHORS. Published by Elsevier Inc on behalf of the Korean Society for Molecular and Cellular Biology. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}