{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T11:55:59Z","timestamp":1782734159467,"version":"3.54.5"},"reference-count":0,"publisher":"Journal of Biological Methods","issue":"2","license":[{"start":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T00:00:00Z","timestamp":1744243200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JBM"],"abstract":"<jats:p>Background: Protein A chromatography is widely used for antibody purification. With conventional packed-bed columns, mass transfer within resin beads is diffusion-limited, entailing long residence time to achieve high binding capacities. Recently, several vendors have introduced Protein A membranes as alternatives to traditional Protein A resins\/columns. These membranes feature open pore structures that facilitate the convective transport of protein molecules, enabling high binding capacities within significantly shorter residence time. The use of Protein A membranes can improve throughput, eliminate the need for column packing, and reduce cost. These advantages notwithstanding, Protein A membranes present certain drawbacks. A major limitation is their high dead volume-to-stationary phase ratio, which leads to larger elution volumes compared to their conventional counterparts. This results in significant eluate dilution and increased buffer consumption. Objective: In the current study, we aimed to demonstrate that ultrafiltration (UF), when used in combination with Protein A membrane chromatography, can address these limitations by allowing eluate concentration and buffer reuse. Methods: A laboratory model of UF integrated Protein A membrane was set up to test the feasibility and effectiveness of the proposed strategy. Results: Integrated UF effectively concentrated Protein A membrane eluate to a concentration comparable to that of Protein A column eluate. In addition, reuse of pH-adjusted UF filtrate as elution buffer reduces buffer consumption by 50%. Conclusion: UF integration is an effective solution for addressing the problem of increased elution volume and buffer consumption associated with Protein A membrane.<\/jats:p>","DOI":"10.14440\/jbm.2025.0109","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T01:54:36Z","timestamp":1744682076000},"page":"e99010050","source":"Crossref","is-referenced-by-count":5,"title":["Incorporating ultrafiltration into Protein A membrane chromatography as a strategy to reduce elution volume and buffer consumption"],"prefix":"10.14440","volume":"12","author":[{"given":"Gaoya","family":"Yuan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xudong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifeng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5574","published-online":{"date-parts":[[2025,4,10]]},"container-title":["Journal of Biological Methods"],"original-title":[],"link":[{"URL":"https:\/\/web-api.polscientific.com\/uploads\/file\/asp\/202505301418480860d3275.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/web-api.polscientific.com\/uploads\/file\/asp\/202505301418480860d3275.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T05:31:46Z","timestamp":1749101506000},"score":1,"resource":{"primary":{"URL":"https:\/\/polscientific.com\/journal\/JBM\/12\/2\/10.14440\/jbm.2025.0109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,10]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,5,30]]}},"URL":"https:\/\/doi.org\/10.14440\/jbm.2025.0109","relation":{},"ISSN":["2326-9901"],"issn-type":[{"value":"2326-9901","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,10]]}}}