{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T02:22:54Z","timestamp":1793931774162,"version":"4.8.0"},"reference-count":0,"publisher":"Journal of Biological Methods","issue":"2","license":[{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JBM"],"abstract":"<jats:p>Background: Early pathogen identification in the bloodstream has long been a key focus for microbiologists and clinicians, given its crucial role in patient management. Matrix-assisted laser desorption ionization-time of flight mass spectrometry has emerged as a valuable tool for the direct and rapid identification of organisms from positive blood cultures. Objective: This study aimed to evaluate the accuracy, productivity, and feasibility of two methods for the rapid detection of bloodstream infections. Methods: Two methods were employed in this study: One based on differential centrifugation and the other using a lysis buffer. Results: The addition of a lysis buffer, sodium dodecyl sulfate (SDS), to the blood culture broth resulted in the identification of a greater number of microorganisms (Acinetobacter baumannii, Pseudomonas aeruginosa, and Klebsiella pneumonia). Conclusion: The application of SDS into culture broths is user-friendly and can be easily integrated into routine blood culture processing, allowing for species-level identification within hours of a positive BacT\/ALERT signal.<\/jats:p>","DOI":"10.14440\/jbm.2025.0119","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T09:52:10Z","timestamp":1741945930000},"page":"e99010058","source":"Crossref","is-referenced-by-count":1,"title":["Bacterial identification using MALDI-TOF mass spectrometry in positive blood cultures: A pilot study"],"prefix":"10.14440","volume":"12","author":[{"given":"Smriti","family":"Srivastava","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akshay","family":"Shankar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neha","family":"Sharad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aparna","family":"Ningombam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamran","family":"Farooque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Purva","family":"Mathur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5574","published-online":{"date-parts":[[2025,3,12]]},"container-title":["Journal of Biological Methods"],"original-title":[],"link":[{"URL":"https:\/\/web-api.polscientific.com\/uploads\/file\/asp\/202505301420321f25f6920.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/web-api.polscientific.com\/uploads\/file\/asp\/202505301420321f25f6920.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T05:37:35Z","timestamp":1749101855000},"score":1,"resource":{"primary":{"URL":"https:\/\/polscientific.com\/journal\/JBM\/12\/2\/10.14440\/jbm.2025.0119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,12]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,5,30]]}},"URL":"https:\/\/doi.org\/10.14440\/jbm.2025.0119","relation":{},"ISSN":["2326-9901"],"issn-type":[{"value":"2326-9901","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,12]]}}}