{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:16:01Z","timestamp":1779963361734,"version":"3.53.1"},"reference-count":28,"publisher":"Charles University in Prague, Karolinum Press","issue":"3","license":[{"start":{"date-parts":[[2016,8,29]],"date-time":"2016-08-29T00:00:00Z","timestamp":1472428800000},"content-version":"vor","delay-in-days":241,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,8,29]],"date-time":"2016-08-29T00:00:00Z","timestamp":1472428800000},"content-version":"tdm","delay-in-days":241,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007397","name":"Univerzita Karlova v Praze","doi-asserted-by":"publisher","award":["PRVOUK P37\/11"],"award-info":[{"award-number":["PRVOUK P37\/11"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007397","name":"Univerzita Karlova v Praze","doi-asserted-by":"publisher","award":["P37\/09"],"award-info":[{"award-number":["P37\/09"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Med. (Hradec Kralove, Czech Repub.)"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>\n                    Goeckerman therapy (GT) represents an effective treatment of psoriasis including a combination of pharmaceutical grade crude coal tar (CCT) and ultraviolet irradiation (UV-R). Coal tar contains a mixture of polycyclic aromatic hydrocarbons. The best known carcinogenic polyaromate \u2013 benzo[a]pyrene is metabolized into a highly reactive benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE). Glutathione S-transferase M1 (GSTM1) catalyses the conjugation of drugs, toxins and products of oxidative stress with glutathione. The aim of the study is to found possible associations between\n                    <jats:italic>GSTM1<\/jats:italic>\n                    genotypes and the level of BPDE-DNA adducts in 46 psoriatic patients treated with GT. For genotyping, droplet digital PCR was applied. The\n                    <jats:italic>GSTM1<\/jats:italic>\n                    copy number was normalized to \u03b2-globin reference gene. In five\n                    <jats:italic>GSTM1*1\/*1<\/jats:italic>\n                    subjects, the\n                    <jats:italic>GSTM1<\/jats:italic>\n                    to \u03b2-globin ratio moved from 0.99 to 1.03 with a median of 1.01.\n                    <jats:italic>GSTM1*0\/*1<\/jats:italic>\n                    heterozygotes (n = 20) contained only one\n                    <jats:italic>GSTM1<\/jats:italic>\n                    function allele which conditioned the ratio 0.47\u20130.53 (median 0.50).\n                    <jats:italic>GSTM1*0\/*0<\/jats:italic>\n                    individuals (n = 21) showed no amplification of the null variants because of the large deletion in\n                    <jats:italic>GSTM1<\/jats:italic>\n                    . BPDE-DNA concentrations ranged from 1.8 to 66.3 ng\/\u00b5g with a median of 12.3 ng\/\u00b5g.\n                    <jats:italic>GSTM1*0\/*0<\/jats:italic>\n                    and\n                    <jats:italic>GSTM1*0\/*1<\/jats:italic>\n                    genotypes showed non-significantly higher concentrations of BPDE-DNA adducts than the\n                    <jats:italic>GSTM1*1\/*1<\/jats:italic>\n                    one (12.3 and 12.4 vs 7.8 ng\/\u00b5g). The non-significant relationship between BPDE-DNA adducts and\n                    <jats:italic>GSTM1<\/jats:italic>\n                    genotypes in psoriatic patients could be associated with relatively low doses of CCT and short-term UV-R exposures used in GT.\n                  <\/jats:p>","DOI":"10.14712\/18059694.2016.94","type":"journal-article","created":{"date-parts":[[2016,8,29]],"date-time":"2016-08-29T17:32:28Z","timestamp":1472491948000},"page":"75-78","source":"Crossref","is-referenced-by-count":0,"title":["Droplet Digital PCR Analysis of GSTM1 Deletion Polymorphism in Psoriatic Subjects Treated with Goeckerman 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