{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:08:04Z","timestamp":1779962884817,"version":"3.53.1"},"reference-count":34,"publisher":"Charles University in Prague, Karolinum Press","issue":"3","license":[{"start":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:00:00Z","timestamp":1675296000000},"content-version":"vor","delay-in-days":397,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:00:00Z","timestamp":1675296000000},"content-version":"tdm","delay-in-days":397,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["actamedica.lfhk.cuni.cz"],"crossmark-restriction":true},"short-container-title":["Acta Med. (Hradec Kralove, Czech Repub.)"],"published-print":{"date-parts":[[2022]]},"abstract":"<jats:p>\n                    Introduction: Genetic mechanisms among many other factors play a crucial role in the development and progression of nonalcoholic fatty liver disease (NAFLD). The farnesoid X-receptor (FXR) regulates the expression of target genes involved in metabolic and energy homeostasis, so it can be assumed that genetic variations within the\n                    <jats:italic>NR1H4<\/jats:italic>\n                    gene, encoding FXR, can affect the development or progression of associated diseases, including NAFLD. The aim: To study the association of SNP rs11110390\n                    <jats:italic>NR1H4<\/jats:italic>\n                    gene with the probability of development and course of NAFLD in children. Materials and methods: 76 children aged 9\u201317 years and overweight were examined. According to controlled attenuated parameter (CAP) measurement (Fibroscan\u00ae502touch) children were divided into 2 groups: group 1 consisted of 40 patients with NAFLD, group 2 was composed by 36 patients without hepatic steatosis. According to genetic testing children were divided into 3 subgroups \u2013 children with CC-, CT-, TT-genotype SNP rs11110390\n                    <jats:italic>NR1H4<\/jats:italic>\n                    gene. Results: The frequency of TT-genotype SNP rs11110390\n                    <jats:italic>NR1H4<\/jats:italic>\n                    gene detection in children with NAFLD was 17.5% versus 2.8% in the control group (p &lt; 0.05). In children with TT-genotype SNP rs11110390\n                    <jats:italic>NR1H4<\/jats:italic>\n                    gene the liver stiffness (p &lt; 0.05) and CAP (p = 0.1) were higher than in patients with CC- and CT-genotypes. Patients with the TT-genotype differed from CC-genotype patients with lower levels of IL-10 (p &lt; 0.05) and pro-inflammatory cytokine balance (p &lt; 0.05). An increase in the concentration of taurine-conjugated bile acid fractions in the hepatic and gallbladder\u2019s bile in children with TT-genotype SNP rs11110390\n                    <jats:italic>NR1H4<\/jats:italic>\n                    (p &lt; 0.05) was demonstrated. Conclusions: SNP rs11110390\n                    <jats:italic>NR1H4<\/jats:italic>\n                    is associated with an increased probability of NAFLD development in children. An increase in the steatosis degree and liver stiffness in combination with increased taurine-conjugated bile acids fractions in the hepatic and gallbladder\u2019s bile, shift in cytokine balance due to a decrease in IL-10 level in children with TT-genotype SNP rs11110390\n                    <jats:italic>NR1H4<\/jats:italic>\n                    were observed.\n                  <\/jats:p>","DOI":"10.14712\/18059694.2022.26","type":"journal-article","created":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T15:48:23Z","timestamp":1675439303000},"page":"105-111","update-policy":"https:\/\/doi.org\/10.14712\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["The Role of FXR-Signaling Variability in the Development and Course of Non-Alcoholic Fatty Liver Disease in Children"],"prefix":"10.14712","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6721-2468","authenticated-orcid":false,"given":"Yuriy","family":"Stepanov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5821-1351","authenticated-orcid":false,"given":"Natalia","family":"Zavhorodnia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5878-179X","authenticated-orcid":false,"given":"Inna","family":"Klenina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6688-6623","authenticated-orcid":false,"given":"Olena","family":"Hrabovska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1738-4624","authenticated-orcid":false,"given":"Viktoria","family":"Yagmur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2023,2,2]]},"reference":[{"key":"18059694.2022.26_1","first-page":"99","volume":"2","author":"Stepanov","year":"2015","journal-title":"Gastroenterologia"},{"key":"18059694.2022.26_2","doi-asserted-by":"crossref","first-page":"629","DOI":"10.21037\/hbsn.2019.09.15","volume":"8","author":"Mantovani","year":"2019","journal-title":"Hepatobiliary Surg Nutr"},{"key":"18059694.2022.26_3","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1016\/j.bbadis.2014.04.025","volume":"1842","author":"van der Sijde","year":"2014","journal-title":"Biochim Biophys Acta"},{"key":"18059694.2022.26_4","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1053\/j.gastro.2020.01.048","volume":"158","author":"Goldner","year":"2020","journal-title":"Gastroenterology"},{"key":"18059694.2022.26_5","doi-asserted-by":"crossref","first-page":"e13331","DOI":"10.1111\/eci.13331","volume":"50","author":"Ismaiel","year":"2020","journal-title":"Eur J Clin Invest"},{"key":"18059694.2022.26_6","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1016\/j.dld.2019.05.029","volume":"51","author":"Zusi","year":"2019","journal-title":"Dig Liver Dis"},{"key":"18059694.2022.26_7","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1002\/hep.31229","volume":"72","author":"Sookoian","year":"2020","journal-title":"Hepatology"},{"key":"18059694.2022.26_8","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s12519-011-0299-z","volume":"8","author":"Chen","year":"2012","journal-title":"World J Pediatr"},{"key":"18059694.2022.26_9","doi-asserted-by":"crossref","first-page":"10713","DOI":"10.1038\/ncomms10713","volume":"7","author":"Gomez-Ospina","year":"2016","journal-title":"Nat Commun"},{"key":"18059694.2022.26_10","first-page":"187","volume":"23","author":"Pavlovic","year":"2017","journal-title":"Curr Pharm Des"},{"key":"18059694.2022.26_11","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1002\/cphy.c190007","volume":"10","author":"Ticho","year":"2019","journal-title":"Compr Physiol"},{"key":"18059694.2022.26_12","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1517\/17425255.2014.999664","volume":"11","author":"Koutsounas","year":"2015","journal-title":"Expert Opin Drug Metab Toxicol"},{"key":"18059694.2022.26_13","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1016\/j.biotechadv.2018.03.003","volume":"36","author":"Hiebl","year":"2018","journal-title":"Biotechnol Adv"},{"key":"18059694.2022.26_14","first-page":"176","volume":"3","author":"Veselsky","year":"1979","journal-title":"Laboratory Work"},{"key":"18059694.2022.26_15","unstructured":"National Center for Biotechnology Information U.S. National Library of Medicine. 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