{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:10:48Z","timestamp":1779963048499,"version":"3.53.1"},"reference-count":22,"publisher":"Charles University in Prague, Karolinum Press","issue":"1","license":[{"start":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T00:00:00Z","timestamp":1523923200000},"content-version":"vor","delay-in-days":106,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T00:00:00Z","timestamp":1523923200000},"content-version":"tdm","delay-in-days":106,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["00023884"],"award-info":[{"award-number":["00023884"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["IG144102"],"award-info":[{"award-number":["IG144102"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prague Med. Rep."],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>\n                    The current diagnostic algorithm for beta-lactam allergy is based on skin and provocation tests, both of which carry a certain risk of inducing hypersensitivity reactions. Thus, non-invasive\n                    <jats:italic>in vitro<\/jats:italic>\n                    tests reliable enough to replace skin and provocation tests at least in a portion of patients are desirable. We aimed to verify the utility of IFN-\u03b3 ELISPOT as a first-line test in patients with suspected non-immediate hypersensitivity reaction to amoxicillin (AMX) and penicillin (PNC). The prospective observational study included 24 patients with recent, suspected non-immediate hypersensitivity reaction to AMX or PNC and 6 recently-exposed healthy subjects.\n                    <jats:italic>In vitro<\/jats:italic>\n                    tests were performed in all patients and healthy subjects: a) IFN-\u03b3 ELISPOT with PNC, AMX and amoxicillin plus clavulanic acid (AMX-CL); b) penicillin specific IgE; c) basophil activation test (BAT). Skin and provocation tests followed only in certain patients. IFN-\u03b3 ELISPOT results with PNC and AMX stimulation did not differ from the unstimulated condition. The highest IFN-\u03b3 responses to AMX-CL were close to previously published criteria in three patients; one of which had true hypersensitivity according to drug provocation tests. Five patients with confirmed hypersensitivity by skin tests showed no response to the culprit antibiotic on IFN-\u03b3 ELISPOT assay. Our results did not support the utility of IFN-\u03b3 ELISPOT in the diagnosis of mild, non-immediate hypersensitivity to amoxicillin and penicillin.\n                  <\/jats:p>","DOI":"10.14712\/23362936.2018.3","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T18:34:46Z","timestamp":1523990086000},"page":"30-42","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of IFN-\u03b3 Enzyme-linked Immunospot Assay (ELISPOT) as a First-line Test in the Diagnosis of Non-Immediate Hypersensitivity to Amoxicillin and Penicillin"],"prefix":"10.14712","volume":"119","author":[{"given":"Lenka","family":"Sedl\u00e1\u010dkov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslav","family":"Pr\u016fcha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Pol\u00e1kov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanka","family":"M\u00edkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2018,4,17]]},"reference":[{"key":"23362936.2018.3_1","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.jaci.2005.10.030","volume":"117","author":"Beeler","year":"2006","journal-title":"J. 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