{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:15:25Z","timestamp":1779963325088,"version":"3.53.1"},"reference-count":16,"publisher":"Charles University in Prague, Karolinum Press","issue":"3","license":[{"start":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T00:00:00Z","timestamp":1763164800000},"content-version":"vor","delay-in-days":9450,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T00:00:00Z","timestamp":1763164800000},"content-version":"tdm","delay-in-days":9450,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["304\/97\/1072"],"award-info":[{"award-number":["304\/97\/1072"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB 367\/C1"],"award-info":[{"award-number":["SFB 367\/C1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fol. Biol."],"published-print":{"date-parts":[[2000]]},"abstract":"<jats:p>\n                    The granulomatous reaction accompanied with MGC formation represents the most striking feature of the non-favourable biological tolerance of implanted devices. We compared MGC formation in the course of the granulomatous reaction\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    employing three types of hydrogels whose biocompatibility had been well studied earlier. The efficiency of the\n                    <jats:italic>in vitro<\/jats:italic>\n                    assay for the granulomatous reaction, including MGC formation, was verified employing the nematode\n                    <jats:italic>Nippostrongylus brasiliensis<\/jats:italic>\n                    , a well-known inductor of MGC formation\n                    <jats:italic>in vitro<\/jats:italic>\n                    . The\n                    <jats:italic>in vitro<\/jats:italic>\n                    results demonstrated a very low level of MGC formation in reaction against all three types of hydrogels without polymer-specific differences in comparison with the nematode experiment characterized by a high extent of MGC formation. On the other hand, the extent of MGC formation was implant type-specific\n                    <jats:italic>in vivo<\/jats:italic>\n                    : pHEMA-\n                    <jats:italic>co<\/jats:italic>\n                    -DMAEMA &gt; pHEMA &gt; pHEMA-\n                    <jats:italic>co<\/jats:italic>\n                    -NaMA. These results indicate that in the\n                    <jats:italic>in vitro<\/jats:italic>\n                    assay it was not possible to discriminate among the types of polymers used in the experiment in comparison with the animal experiment. They also indicate potential differences between granuloma formation induced by parasites and by foreign bodies.\n                  <\/jats:p>","DOI":"10.14712\/fb2000046030113","type":"journal-article","created":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T10:02:41Z","timestamp":1763200961000},"page":"113-118","source":"Crossref","is-referenced-by-count":0,"title":["Synthetic Hydrogel Capacity to Induce Formation of Foreign-Body Giant Multinucleate Cells Differs in Vivo and in Vitro"],"prefix":"10.14712","volume":"46","author":[{"suffix":"Jr.","given":"Karel","family":"Smetana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Hol\u00edkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Seitzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Haas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Vac\u00edk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2025,11,15]]},"reference":[{"key":"fb2000046030113_1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1002\/jbm.820080503","volume":"8","author":"Coleman","year":"1974","journal-title":"J. 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