{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:16:44Z","timestamp":1779963404978,"version":"3.53.1"},"reference-count":28,"publisher":"Charles University in Prague, Karolinum Press","issue":"2","license":[{"start":{"date-parts":[[2025,1,12]],"date-time":"2025-01-12T00:00:00Z","timestamp":1736640000000},"content-version":"vor","delay-in-days":6586,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,12]],"date-time":"2025-01-12T00:00:00Z","timestamp":1736640000000},"content-version":"tdm","delay-in-days":6586,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fol. Biol."],"published-print":{"date-parts":[[2007]]},"abstract":"<jats:p>\n                    A 3D culture system was used to investigate the behaviour of mesothelial cells present in the wall of human\n                    <jats:italic>processus vaginalis peritonei<\/jats:italic>\n                    . Small tissue fragments placed on collagen sponges were cultured for 7, 14 and 21 days in medium supplemented with 10% FBS, and analysed for the expression and distribution of cytokeratins (CKAE1-AE3, CK19), p63, Ki-67, vimentin, CD34, and HBME-1. Before culture, flat mesothelial cells displayed immunoreactivity for cytokeratins, vimentin and HBME-1, while p63 and CD34 were negative. Mesenchymal cells within the stroma were vimentin-positive and endothelial cells of small vessels displayed positive staining for CD34. Cytokeratins, p63 and HBME-1 were negative in all stromal cells. In cultured fragments, flat mesothelial cells positive for vimentin, cytokeratins and HBME-1 proliferated, lining the fragment surface and migrating into the sponge. Capillaries showed morphological alterations; however, their immunoreactivity was comparable with the stroma prior to culture. Cells that had migrated into the sponge and displayed characteristics of mesothelial progenitors, predominantly spindle-shaped and stellate, showed heterogeneous expression of markers especially in late phases of cultivation. These cells were constantly positive for vimentin, a small fraction was cytokeratin-positive and a few displayed HBME-1 immunoreactivity. CD34 was found in cells forming small cavities into the matrix, resembling newly formed blood vessels. Cells that had migrated into the sponge could be isolated and expanded in co-culture with feeder NIH.3T3 fibroblasts. This system is suitable for studying growth and behaviour of mesothelial cells within their natural environment, providing a good method for isolation and expansion of their progenitor cells.\n                  <\/jats:p>","DOI":"10.14712\/fb2007053020050","type":"journal-article","created":{"date-parts":[[2025,1,12]],"date-time":"2025-01-12T14:55:51Z","timestamp":1736693751000},"page":"50-57","source":"Crossref","is-referenced-by-count":2,"title":["Expansion of Human Mesothelial Progenitor Cells in a Long- term Three-dimensional Organotypic Culture of Processus Vaginalis Peritonei"],"prefix":"10.14712","volume":"53","author":[{"given":"A.","family":"Rosellini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Michelini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Tanda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Mandys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto P.","family":"Revoltella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2025,1,12]]},"reference":[{"key":"fb2007053020050_1","doi-asserted-by":"crossref","first-page":"2629","DOI":"10.1089\/ten.2006.12.2629","volume":"12","author":"Asano","year":"2006","journal-title":"Tissue Eng."},{"key":"fb2007053020050_2","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1046\/j.1365-2559.2000.00981.x","volume":"37","author":"Attanoos","year":"2000","journal-title":"Histopathology"},{"key":"fb2007053020050_3","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/j.yexcr.2005.11.028","volume":"312","author":"Barbieri","year":"2006","journal-title":"Exp. 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