{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:11:37Z","timestamp":1779963097522,"version":"3.53.1"},"reference-count":35,"publisher":"Charles University in Prague, Karolinum Press","issue":"4","license":[{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"vor","delay-in-days":4611,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"tdm","delay-in-days":4611,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fol. Biol."],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>\n                    The genus\n                    <jats:italic>Holoaden<\/jats:italic>\n                    includes three species described so far, but the only published cytogenetic data is from\n                    <jats:italic>Holoaden bradei<\/jats:italic>\n                    , with the karyotype 2n = 18, based on conventional staining. In the present paper we report, for the first time, data on chromosomes of\n                    <jats:italic>H. luederwaldti<\/jats:italic>\n                    , which presented 2n = 18 and a case of natural triploidy, with 2n = 3x = 27. In this sample, another karyotypic variation was observed due to the occurrence of two types of chromosome 8, which present submetacentric or subtelocentric morphologies. Homomorphic subtelocentric or heteromorphic condition was observed among the diploid specimens, whereas the triploid had one submetacentric and two subtelocentric chromosomes 8. In all specimens, Ag-NOR was located in the long arms of chromosomes 8, at the interstitial region when subtelocentric, or in the proximal region when submetacentric, confirmed by fluorescent\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization with the HM123 probe. The C bands showed centromeric distribution and distribution at Ag-NOR site. The centromeric heterochromatin was fluorescent with DAPI staining, whereas the AgNOR displayed bright fluorescence with CMA\n                    <jats:sub>3<\/jats:sub>\n                    . Fluorescent\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization using a telomeric probe labelled exclusively the telomere regions. Although the same 2n = 18 chromosome numbers have been observed in\n                    <jats:italic>H. luederwaldti<\/jats:italic>\n                    and\n                    <jats:italic>H. bradei<\/jats:italic>\n                    , some differences in both karyotypes can be visualized, mainly with regard to the morphology of the last chromosome pairs.\n                  <\/jats:p>","DOI":"10.14712\/fb2012058040144","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T06:59:57Z","timestamp":1723791597000},"page":"144-150","source":"Crossref","is-referenced-by-count":3,"title":["The Karyotype of Holoaden luederwaldti (Anura, Strabomantidae), with Report of Natural Triploidy"],"prefix":"10.14712","volume":"58","author":[{"given":"Jo\u00e3o R. C.","family":"Campos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. A.","family":"Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. F. B.","family":"Haddad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Kasahara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2024,8,16]]},"reference":[{"key":"fb2012058040144_1","first-page":"335","volume":"16","author":"Baldissera","year":"1993","journal-title":"Rev. Brasil. Genet."},{"key":"fb2012058040144_2","unstructured":"Batistic, R. F. (1989) Aspectos citogen\u00e9ticos da evolu\u00e7\u00e3o em Phyllomedusa (Anura Amphibia). PhD Thesis \u2013 USP, S\u00e3o Paulo, Brazil, 1-203."},{"key":"fb2012058040144_3","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1007\/BF00293683","volume":"19","author":"Be\u00e7ak","year":"1966","journal-title":"Chromosoma"},{"key":"fb2012058040144_4","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1139\/g67-057","volume":"9","author":"Bogart","year":"1967","journal-title":"Can. J. Genet. Cytol."},{"key":"fb2012058040144_5","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1159\/000130172","volume":"11","author":"Bogart","year":"1972","journal-title":"Cytogenetics"},{"key":"fb2012058040144_6","first-page":"152","volume":"3","author":"Borkin","year":"1996","journal-title":"Russ. J. Herpetol."},{"key":"fb2012058040144_7","first-page":"121","volume":"14","author":"Borkin","year":"2007","journal-title":"Russ. J. Herpetol."},{"key":"fb2012058040144_8","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.micron.2006.11.001","volume":"39","author":"Campos","year":"2008","journal-title":"Micron"},{"key":"fb2012058040144_9","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1159\/000015093","volume":"82","author":"Christian","year":"1998","journal-title":"Cytogenet. Cell Genet."},{"key":"fb2012058040144_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1206\/0003-0090(2005)294[0001:SROTFF]2.0.CO;2","volume":"294","author":"Faivovich","year":"2005","journal-title":"Bull. Am. Mus. Nat. Hist."},{"key":"fb2012058040144_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1206\/0003-0090(2006)297[0001:TATOL]2.0.CO;2","volume":"297","author":"Frost","year":"2006","journal-title":"Bull. Am. Mus. Nat. Hist."},{"key":"fb2012058040144_12","unstructured":"Frost, D. R. (2011) Amphibian species of the world: an online reference. Version 5.5 (31 January, 2011). 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