{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:12:51Z","timestamp":1779963171585,"version":"3.53.1"},"reference-count":28,"publisher":"Charles University in Prague, Karolinum Press","issue":"3","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"vor","delay-in-days":3834,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"tdm","delay-in-days":3834,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["IN 00023001"],"award-info":[{"award-number":["IN 00023001"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007543","name":"Grantov\u00e1 Agentura, Univerzita Karlova","doi-asserted-by":"publisher","award":["GAUK-251168_99910"],"award-info":[{"award-number":["GAUK-251168_99910"]}],"id":[{"id":"10.13039\/100007543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007397","name":"Univerzita Karlova v Praze","doi-asserted-by":"publisher","award":["SVV2013\/266504"],"award-info":[{"award-number":["SVV2013\/266504"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["VFN, 64165"],"award-info":[{"award-number":["VFN, 64165"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fol. Biol."],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>\n                    <jats:italic>OTC<\/jats:italic>\n                    encodes ornithine carbamoyltransferase, mitochondrial matrix enzyme involved in the synthesis of urea. The tissue-specific expression of\n                    <jats:italic>OTC<\/jats:italic>\n                    in the liver and intestine is dependent on the interaction of\n                    <jats:italic>OTC<\/jats:italic>\n                    promoter with an upstream enhancer. HNF-4 and C\/EBP\u03b2 are crucial for this interaction in the rat and mouse. In the present study we focused on characterization of elements involved in the regulation of OTC transcription in human. Using a set of 5\u2019-deleted promoter mutants in a reporter assay we identified two positive cis-acting regulatory elements located at c.-105 and c.-136 within the human\n                    <jats:italic>OTC<\/jats:italic>\n                    promoter. Both are essential for the transcriptional activity of the promoter itself and for the interaction with the enhancer. Protein binding at the corresponding sites was confirmed by DNase I footprinting. Electromobility shift assay with a specific competitor and anti-HNF-4\u03b1 antibody identified the DNA-protein binding sites as HNF-4\u03b1 recognition motifs. A third HNF-4\u03b1 binding site has been found at the position c.-187. All three HNF-4\u03b1 binding sites are located within 35 bp upstream of the transcription start sites at positions c.-95, c.-119 (major) and c.-169 (minor). A series of C\/EBP\u03b2 recognition motifs was identified within the enhancer. Involvement of C\/EBP\u03b2 and HNF-4\u03b1 in the promoter-enhancer interaction is further supported by a massive DNA-protein interaction observed in the footprinting and EMSA assays. Since the\n                    <jats:italic>OTC<\/jats:italic>\n                    promoter lacks general core promoter elements such as TATA-box or initiators in standard positions, HNF-4\u03b1 most likely plays an essential role in the initiation of\n                    <jats:italic>OTC<\/jats:italic>\n                    transcription in human.\n                  <\/jats:p>","DOI":"10.14712\/fb2014060030133","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T05:32:57Z","timestamp":1719811977000},"page":"133-143","source":"Crossref","is-referenced-by-count":2,"title":["HNF-4\u03b1 Regulates Expression of Human Ornithin Carbamoyltransferase through Interaction with Two Positive Cis-Acting Regulatory Elements Located in the Proximal Promoter"],"prefix":"10.14712","volume":"60","author":[{"given":"O.","family":"Luk\u0161an","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Dvo\u0159\u00e1kov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milan","family":"Jirsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2024,7,1]]},"reference":[{"key":"fb2014060030133_1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1002\/jcp.1111","volume":"188","author":"Black","year":"2001","journal-title":"J. Cell. Physiol."},{"key":"fb2014060030133_2","unstructured":"Brusilow, S. W., Horwich, A. L. (1995) Urea cycle enzymes. In The Molecular and Metabolic Bases of Inherited Disease, eds. R. Scriver, A. L. Beaudet, W. S. Sly, D. Valle, pp. 1187-1232, McGraw-Hill, New York."},{"key":"fb2014060030133_3","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1128\/MCB.16.3.925","volume":"16","author":"Drewes","year":"1996","journal-title":"Mol. Cell. Biol."},{"key":"fb2014060030133_4","doi-asserted-by":"crossref","first-page":"5343","DOI":"10.1093\/nar\/gks190","volume":"40","author":"Fang","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"fb2014060030133_5","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1038\/nsmb763","volume":"11","author":"Hahn","year":"2004","journal-title":"Nat. Struct. Mol. 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Tech."}],"container-title":["Folia Biologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/foliabiologica.lf1.cuni.cz\/media\/pdf\/fb_2014060030133.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/foliabiologica.lf1.cuni.cz\/media\/pdf\/fb_2014060030133.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:43:50Z","timestamp":1779961430000},"score":1,"resource":{"primary":{"URL":"https:\/\/foliabiologica.lf1.cuni.cz\/60\/3\/0133\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":28,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2014]]}},"alternative-id":["fb_2014060030133"],"URL":"https:\/\/doi.org\/10.14712\/fb2014060030133","relation":{},"ISSN":["0015-5500","2533-7602"],"issn-type":[{"value":"0015-5500","type":"print"},{"value":"2533-7602","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}