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Biol."],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>\n                    Natural proteins can be used in measuring intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration. As one of the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    - regulated photoproteins, aequorin has several advantages in comparison to widely used Ca\n                    <jats:sup>2+<\/jats:sup>\n                    fluorescence indicators (e.g., fura-2, indo-1 and fluo-3), including high dynamic range and resistance to motion artefacts. However, incorporation of aequorin into cells remains a challenge. Hypoosmotic shock treatment was optimized and used as a method for loading aequorin into the cytoplasm of follicular lymphoma cells. Measurement of aequorin luminescence in the cells was performed using a luminometer with a sensitive photomultiplier tube and the luminescence intensity was recalculated into intracellular [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]. The value of (0.85 \u00b1 0.52)\u00b710\n                    <jats:sup>-6<\/jats:sup>\n                    M was found. We show that the optimized method of incorporation was effective for loading aequorin into follicular lymphoma cells\n                    <jats:italic>in vitro<\/jats:italic>\n                    . The cell viability remains high immediately after the procedure. This method can also be used for measuring intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration in other types of non-adherent cells.\n                  <\/jats:p>","DOI":"10.14712\/fb2015061040134","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T10:57:14Z","timestamp":1715252234000},"page":"134-139","source":"Crossref","is-referenced-by-count":4,"title":["Aequorin as Intracellular Ca2+ Indicator Incorporated in Follicular Lymphoma Cells by Hypoosmotic Shock Treatment"],"prefix":"10.14712","volume":"61","author":[{"given":"M.","family":"Klabusay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Skopal\u00edk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Erceg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ale\u0161","family":"Hrdli\u010dka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2024,5,9]]},"reference":[{"key":"fb2015061040134_1","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1126\/science.841325","volume":"195","author":"Allen","year":"1977","journal-title":"Science"},{"key":"fb2015061040134_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0143416002001860","volume":"32","author":"Alvarez","year":"2002","journal-title":"Cell Calcium"},{"key":"fb2015061040134_3","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0304-3940(96)12497-6","volume":"207","author":"Barber","year":"1996","journal-title":"Neurosci. 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