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Biol."],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>\n                    <jats:italic>Bacillus anthracis<\/jats:italic>\n                    is a spore-forming, Grampositive microorganism. It is a causative agent of anthrax, a highly infectious disease. It belongs to the \u201c\n                    <jats:italic>Bacillus cereus<\/jats:italic>\n                    group\u201d, which contains other closely related species, including\n                    <jats:italic>Bacillus cereus<\/jats:italic>\n                    ,\n                    <jats:italic>Bacillus thuringiensis<\/jats:italic>\n                    ,\n                    <jats:italic>Bacillus mycoides<\/jats:italic>\n                    ,\n                    <jats:italic>Bacillus weihenstephanensis<\/jats:italic>\n                    , and\n                    <jats:italic>Bacillus pseudomycoides<\/jats:italic>\n                    .\n                    <jats:italic>B. anthracis<\/jats:italic>\n                    naturally occurs in soil environments. The\n                    <jats:italic>BA5345<\/jats:italic>\n                    genetic marker was used for highly specific detection of\n                    <jats:italic>B. anthracis<\/jats:italic>\n                    with TaqMan probes. The detection limit of a real-time PCR assay was estimated at the level of 16.9 copies (CI\n                    <jats:sub>95%<\/jats:sub>\n                    - 37.4 to 37.86, SD = 0.2; SE = 0.118). Oligonucleotides designed for the targeted sequences (within the tested locus) revealed 100 % homology to\n                    <jats:italic>B. anthracis<\/jats:italic>\n                    strain reference sequences deposited in the database (NCBI) and high specificity to all tested\n                    <jats:italic>B. anthracis<\/jats:italic>\n                    strains. Additional\n                    <jats:italic>in silico<\/jats:italic>\n                    analysis of plasmid markers\n                    <jats:italic>pag<\/jats:italic>\n                    and\n                    <jats:italic>cap<\/jats:italic>\n                    genes with\n                    <jats:italic>B. anthracis<\/jats:italic>\n                    strains included in the database was carried out. Our study clearly indicates that the\n                    <jats:italic>BA5345<\/jats:italic>\n                    marker can be used with success as a chromosomal marker in routine identification of\n                    <jats:italic>B. anthracis<\/jats:italic>\n                    ; moreover, detection of plasmid markers indicates virulence of the examined strains.\n                  <\/jats:p>","DOI":"10.14712\/fb2015061050178","type":"journal-article","created":{"date-parts":[[2024,4,27]],"date-time":"2024-04-27T15:57:09Z","timestamp":1714233429000},"page":"178-183","source":"Crossref","is-referenced-by-count":9,"title":["Real-Time PCR Identification of Unique Bacillus anthracis Sequences"],"prefix":"10.14712","volume":"61","author":[{"given":"P.","family":"Cie\u015blik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Knap","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ko\u0142odziej","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Mirski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Joniec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Graniak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"\u017bakowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Winnicka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Bielawska-Dr\u00f3zd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2024,4,27]]},"reference":[{"key":"fb2015061050178_1","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.mcp.2008.06.001","volume":"22","author":"Antwerpen","year":"2008","journal-title":"Mol. 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