{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:16:47Z","timestamp":1779963407733,"version":"3.53.1"},"reference-count":29,"publisher":"Charles University in Prague, Karolinum Press","issue":"5","license":[{"start":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:00:00Z","timestamp":1704844800000},"content-version":"vor","delay-in-days":2200,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:00:00Z","timestamp":1704844800000},"content-version":"tdm","delay-in-days":2200,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018628","name":"Scientific Research Foundation of Education Department of Anhui Province of China","doi-asserted-by":"publisher","award":["1604a0802078"],"award-info":[{"award-number":["1604a0802078"]}],"id":[{"id":"10.13039\/501100018628","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fol. Biol."],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>\n                    Tumour necrosis factor-stimulated gene 6 (\n                    <jats:italic>TSG6<\/jats:italic>\n                    ) is a protective inflammatory reaction gene which is upregulated by inflammatory processes. Recent studies suggest that TSG-6 exhibits anti-scarring effects. However, the mechanism of TSG-6 action in the scar formation remains poorly understood. We investigated whether TSG-6 affects growth of the human hypertrophic scar fibroblasts (HSFs) via Fas\/FasL signalling pathway. Cultured HSFs were transfected with a vector carrying the\n                    <jats:italic>TSG6<\/jats:italic>\n                    gene (pLVX-Puro-TSG-6) or with a vector not containing the\n                    <jats:italic>TSG6<\/jats:italic>\n                    gene (pLVX-Puro). Untransfected HSFs served as a control group to both transfected HSFs. The expressions level of TSG-6 was up-regulated in the pLVX-Puro-TSG-6 group at the protein and mRNA level. MTT and flow cytometry were used to assess the effect of TSG-6 on the growth and apoptotic status of HSFs. Finally, qRT-PCR and western blot were used to measure the expression levels of Fas, FasL, FADD, caspase-3 and caspase-8 in each group. The apoptosis rate was significantly enhanced and the growth rate reduced in the HSFs transfected with the\n                    <jats:italic>TSG6<\/jats:italic>\n                    gene vector. The expression levels of Fas, FasL, FADD, caspase-3 and caspase- 8 were significantly raised in the TSG-6 overexpressing HSFs. It is concluded that increased expression of TSG-6 may induce apoptosis of human hypertrophic scar fibroblasts via activation of the Fas\/FasL signalling pathway.\n                  <\/jats:p>","DOI":"10.14712\/fb2018064050173","type":"journal-article","created":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T16:46:30Z","timestamp":1704905190000},"page":"173-181","source":"Crossref","is-referenced-by-count":6,"title":["TSG-6 Induces Apoptosis of Human Hypertrophic Scar Fibroblasts via Activation of the Fas\/FasL Signalling Pathway"],"prefix":"10.14712","volume":"64","author":[{"given":"X.-Y.","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Jing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.-N.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2024,1,10]]},"reference":[{"key":"fb2018064050173_1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/0014-5793(95)00339-B","volume":"364","author":"Aggarwal","year":"1995","journal-title":"FEBS Lett."},{"key":"fb2018064050173_2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S1044-5323(03)00031-9","volume":"15","author":"Barnhart","year":"2003","journal-title":"Semin. 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