{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:09:26Z","timestamp":1779962966004,"version":"3.53.1"},"reference-count":25,"publisher":"Charles University in Prague, Karolinum Press","issue":"1","license":[{"start":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T00:00:00Z","timestamp":1703808000000},"content-version":"vor","delay-in-days":1823,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T00:00:00Z","timestamp":1703808000000},"content-version":"tdm","delay-in-days":1823,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","award":["LM2015062"],"award-info":[{"award-number":["LM2015062"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["16-02972S"],"award-info":[{"award-number":["16-02972S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["18-03461S"],"award-info":[{"award-number":["18-03461S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","award":["PROGRES-Q38"],"award-info":[{"award-number":["PROGRES-Q38"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","award":["INTER-COST LTC17023"],"award-info":[{"award-number":["INTER-COST LTC17023"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007543","name":"Grantov\u00e1 Agentura, Univerzita Karlova","doi-asserted-by":"publisher","award":["1456217"],"award-info":[{"award-number":["1456217"]}],"id":[{"id":"10.13039\/100007543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007543","name":"Grantov\u00e1 Agentura, Univerzita Karlova","doi-asserted-by":"publisher","award":["OP RDE CZ.02.1.01\/0.0\/0.0\/16_013\/0001775"],"award-info":[{"award-number":["OP RDE CZ.02.1.01\/0.0\/0.0\/16_013\/0001775"]}],"id":[{"id":"10.13039\/100007543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004240","name":"Akademie V\u011bd \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["67985823"],"award-info":[{"award-number":["67985823"]}],"id":[{"id":"10.13039\/501100004240","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fol. Biol."],"published-print":{"date-parts":[[2019]]},"abstract":"<jats:p>\n                    Hypothermic incubation of chicken eggs leads to smaller embryos with enlarged hearts, originally described as hypertrophic. Over the years, however, accumulated evidence suggested that hyperplasia, rather than hypertrophy, is the predominant mechanism of cardiac growth during the prenatal period. We have thus set to re-evaluate the hypothermia model to precise the exact cellular mechanism behind cardiac enlargement. Fertilized chicken eggs were incubated at either 37.5 \u00b0C (normothermia) or 33.5 \u00b0C from embryonic day (ED) 13 onward (hypothermia). Sampling was performed at ED17, at which point wet embryo and heart weight were recorded, and the hearts were submitted to histological examination. In agreement with previous results, the hypothermic embryos were 29% smaller and had hearts 18% larger, translating into a 67% increase in the heart to body weight ratio (P &lt; 0.05 for all parameters). The cell size was essentially the same between control and hypothermic hearts in all regions analysed. Likewise, there was no significant relationship between the cell size and heart weight; however, in the hypothermic hearts, there was a trend showing positive correlation between cell sizes in different cardiac regions and heart weight. Proliferation rate, determined on the basis of anti-phosphohistone H3 immunofluorescence, showed an overall increase in the hypothermic group, reaching statistical significance (P = 0.02,\n                    <jats:italic>t<\/jats:italic>\n                    -test) in the right ventricle. The proliferation rate was similar among different regions of the same heart. However, the correlation between the proliferation rate and heart weight was only small (r\n                    <jats:sup>2<\/jats:sup>\n                    = 0.007 and r\n                    <jats:sup>2<\/jats:sup>\n                    = 0.234 for the normothermic and hypothermic group, respectively). We thus conclude that hyperplasia is the predominant response mechanism in this volume-overload model; mechanistically, decreased heart rate at lower temperature increases the end-diastolic and stroke volume, minimizing the drop in cardiac output through the Frank- Starling mechanism.\n                  <\/jats:p>","DOI":"10.14712\/fb2019065010036","type":"journal-article","created":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T09:26:57Z","timestamp":1703842017000},"page":"36-42","source":"Crossref","is-referenced-by-count":2,"title":["Cardiac Enlargement in the Chick Embryo Induced by Hypothermic Incubation Is Due to a Combination of Hyperplasia and Hypertrophy of Cardiomyocytes"],"prefix":"10.14712","volume":"65","author":[{"given":"K.","family":"Skuhrov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Kvasilov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Svat\u016f\u0148kov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Sedmera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2023,12,29]]},"reference":[{"key":"fb2019065010036_1","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1002\/ar.21280","volume":"294","author":"Benes","year":"2011","journal-title":"Anat. Rec. (Hoboken)"},{"key":"fb2019065010036_2","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1203\/00006450-198911000-00015","volume":"26","author":"Benson","year":"1989","journal-title":"Pediatr. Res."},{"key":"fb2019065010036_3","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1126\/science.1164680","volume":"324","author":"Bergmann","year":"2009","journal-title":"Science"},{"key":"fb2019065010036_4","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/S0022-5223(19)36944-2","volume":"99","author":"Bical","year":"1990","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"fb2019065010036_5","doi-asserted-by":"crossref","first-page":"C97","DOI":"10.1152\/ajpcell.1987.252.1.C97","volume":"252","author":"Boehm","year":"1987","journal-title":"Am. J. Physiol."},{"key":"fb2019065010036_6","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1007\/s10237-012-0461-0","volume":"12","author":"Buffinton","year":"2013","journal-title":"Biomech. Model Mechanobiol."},{"key":"fb2019065010036_7","first-page":"H55","volume":"257","author":"Clark","year":"1989","journal-title":"Am. J. Physiol."},{"key":"fb2019065010036_8","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1007\/BF00319331","volume":"55","author":"Grohmann","year":"1961","journal-title":"Z. f. Zellforschung"},{"key":"fb2019065010036_9","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/0002-9149(78)90812-3","volume":"41","author":"Hutchins","year":"1978","journal-title":"Am. J. Cardiol."},{"key":"fb2019065010036_10","first-page":"405","volume":"25","author":"Jeter","year":"1971","journal-title":"J. Embryol. Exp. Morphol."},{"key":"fb2019065010036_11","doi-asserted-by":"crossref","first-page":"C1091","DOI":"10.1152\/ajpcell.1991.261.6.C1091","volume":"261","author":"Kennedy","year":"1991","journal-title":"Am. J. Physiol."},{"key":"fb2019065010036_12","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1002\/(SICI)1097-0185(199601)244:1<118::AID-AR12>3.0.CO;2-S","volume":"244","author":"Knaapen","year":"1996","journal-title":"Anat. Rec."},{"key":"fb2019065010036_13","doi-asserted-by":"crossref","first-page":"425","DOI":"10.33549\/physiolres.933216","volume":"65","author":"Krejci","year":"2016","journal-title":"Physiol. Res."},{"key":"fb2019065010036_14","unstructured":"Libby, P., Bonow, R. O., Mann, D. L., Zipes, D. P. (2008) Braunwald\u2019s Heart Disease. Elsevier, Philadelphia."},{"key":"fb2019065010036_15","first-page":"975","volume":"50","author":"Merkow","year":"1967","journal-title":"Am. J. Pathol."},{"key":"fb2019065010036_16","doi-asserted-by":"crossref","unstructured":"Ostadal, B. (2013) Comparative aspects of cardiac adaptation. In: Cardiac Adaptations, eds. Ostadal B., Dhalla N. S., pp 3-18. Springer Science+Business Media, New York.","DOI":"10.1007\/978-1-4614-5203-4_1"},{"key":"fb2019065010036_17","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/0890-6238(96)00062-7","volume":"10","author":"Peterka","year":"1996","journal-title":"Reprod. Toxicol."},{"key":"fb2019065010036_18","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1002\/(SICI)1097-0185(199707)248:3<421::AID-AR15>3.0.CO;2-R","volume":"248","author":"Sedmera","year":"1997","journal-title":"Anat. Rec."},{"key":"fb2019065010036_19","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1002\/ar.10098","volume":"267","author":"Sedmera","year":"2002","journal-title":"Anat. Rec."},{"key":"fb2019065010036_20","doi-asserted-by":"crossref","unstructured":"Sedmera, D. (2016) Hemodynamics during development and postnatal life. In: Congenital Heart Diseases: The Broken Heart, eds. Ricken-Sperling, S., Kelly, R. G., Driscoll, D., pp 97-109, Springer Verlag, Wien.","DOI":"10.1007\/978-3-7091-1883-2_9"},{"key":"fb2019065010036_21","first-page":"654","volume":"19","author":"Toussaint","year":"1998","journal-title":"Eur. Heart J."},{"key":"fb2019065010036_22","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.pbiomolbio.2014.06.002","volume":"115","author":"Vostarek","year":"2014","journal-title":"Prog. Biophys. Mol. Biol."},{"key":"fb2019065010036_23","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1111\/apha.12691","volume":"217","author":"Vostarek","year":"2016","journal-title":"Acta Physiol. (Oxf)"},{"key":"fb2019065010036_24","first-page":"281","volume":"25","author":"Warbanow","year":"1970","journal-title":"Acta Biol. Med. Ger."},{"key":"fb2019065010036_25","first-page":"859","volume":"26","author":"Warbanow","year":"1971","journal-title":"Acta Biol. Med. Ger."}],"container-title":["Folia Biologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/foliabiologica.lf1.cuni.cz\/media\/pdf\/fb_2019065010036.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/foliabiologica.lf1.cuni.cz\/media\/pdf\/fb_2019065010036.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:38:36Z","timestamp":1779961116000},"score":1,"resource":{"primary":{"URL":"https:\/\/fb.littledata.cz\/65\/1\/0036\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":25,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019]]}},"alternative-id":["fb_2019065010036"],"URL":"https:\/\/doi.org\/10.14712\/fb2019065010036","relation":{},"ISSN":["0015-5500","2533-7602"],"issn-type":[{"value":"0015-5500","type":"print"},{"value":"2533-7602","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]}}}