{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:06:50Z","timestamp":1779962810902,"version":"3.53.1"},"reference-count":26,"publisher":"Charles University in Prague, Karolinum Press","issue":"5-6","license":[{"start":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T00:00:00Z","timestamp":1698537600000},"content-version":"vor","delay-in-days":1762,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T00:00:00Z","timestamp":1698537600000},"content-version":"tdm","delay-in-days":1762,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["1180"],"award-info":[{"award-number":["1180"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fol. Biol."],"published-print":{"date-parts":[[2019]]},"abstract":"<jats:p>\n                    Several studies have shown that peroxynitrite (ONOO\n                    <jats:sup>\u2013<\/jats:sup>\n                    ), formed upon the reaction of \u2022NO and O\n                    <jats:sup>2\u2013<\/jats:sup>\n                    , is increased in many cardiovascular diseases and is detrimental to myocardial function. Proteins associated with Ca\n                    <jats:sup>2+<\/jats:sup>\n                    homeostasis regulation in the heart may be involved in these effects. Thus, the aim of this study was to elucidate the mechanisms associated with ONOO\n                    <jats:sup>\u2013<\/jats:sup>\n                    -induced effects. We evaluated [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    regulation, sarco\/endoplasmic reticulum Ca\n                    <jats:sup>2+<\/jats:sup>\n                    - binding proteins, and phosphorylation levels of the ryanodine receptor in isolated rat myocytes. Electrical field-induced intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transients and contractions were recorded simultaneously. Myocytes superfused with 3-morpholinosydnonimine N-ethylcarbamide (SIN-1), an ONOO\n                    <jats:sup>\u2013<\/jats:sup>\n                    donor, decreased the amplitude of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transients and contraction in a dose-response (1\u2013200 \u03bcM) manner. Similarly, SIN-1 increased half-time decay in a concentration-dependent manner. Co-infusion of the ONOO\n                    <jats:sup>\u2013<\/jats:sup>\n                    donor with FeTMPyP (1 \u03bcM), an ONOO\n                    <jats:sup>\u2013<\/jats:sup>\n                    decomposition catalyst, inhibited the effects induced by ONOO\n                    <jats:sup>\u2013<\/jats:sup>\n                    . Impaired sarcoplasmic reticulum Ca\n                    <jats:sup>2+<\/jats:sup>\n                    uptake caused by ONOO\n                    <jats:sup>\u2013<\/jats:sup>\n                    (SIN-1 200 \u03bcM) was confirmed by a reduction of caffeine-evoked Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release along with prolongation of the half-time decay. Surprisingly, ONOO\n                    <jats:sup>\u2013<\/jats:sup>\n                    induced a spontaneous Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transient that started at the beginning of the relaxation phase and was inhibited by tetracaine. Also, reduced phosphorylation at the ryanodine receptor 2 (RyR2)-Ser-2814 site was observed. In conclusion, deficient sarco\/endoplasmic reticulum Ca2+-ATPase-mediated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    uptake concomitant with augmented Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release by RyR2 in myocytes may be associated with modification of myocyte Ca\n                    <jats:sup>2+<\/jats:sup>\n                    handling by ONOO\n                    <jats:sup>\u2013<\/jats:sup>\n                    . Thus, development of cardiac failure in diabetes, nephropathy, or hypertension may be related with elevated ONOO\n                    <jats:sup>\u2013<\/jats:sup>\n                    in cardiac tissue.\n                  <\/jats:p>","DOI":"10.14712\/fb2019065050237","type":"journal-article","created":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T13:38:47Z","timestamp":1698586727000},"page":"237-245","source":"Crossref","is-referenced-by-count":1,"title":["Peroxynitrite-Induced Intracellular Ca2+ Depression in Cardiac Myocytes: Role of Sarco\/Endoplasmic Reticulum Ca2+ Pump"],"prefix":"10.14712","volume":"65","author":[{"given":"V.","family":"Flores-Tamez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Escalante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelia","family":"Rios","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2023,10,29]]},"reference":[{"key":"fb2019065050237_1","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.cardiores.2005.03.025","volume":"67","author":"Borbely","year":"2005","journal-title":"Cardiovasc. 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