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Biol."],"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p>\n                    The aim of this\n                    <jats:italic>in vitro<\/jats:italic>\n                    study was to examine the dose-dependent effects of iron as a potential endocrine disruptor in relation to the release of sexual steroid hormones by a human adrenocortical carcinoma (NCI-H295R) cell line. The cells were exposed to different concentrations (3.90, 62.50, 250, 500, 1000 \u03bcM) of FeSO\n                    <jats:sub>4<\/jats:sub>\n                    .7H\n                    <jats:sub>2<\/jats:sub>\n                    O and compared with the control group (culture medium without FeSO\n                    <jats:sub>4<\/jats:sub>\n                    .7H\n                    <jats:sub>2<\/jats:sub>\n                    O). Cell viability was measured by the metabolic activity assay. Quantification of sexual steroid production was performed by enzyme-linked immunosorbent assay. Following 48 h culture of the cells in the presence of FeSO\n                    <jats:sub>4<\/jats:sub>\n                    .7H\n                    <jats:sub>2<\/jats:sub>\n                    O, significantly (P &lt; 0.001) increased production of progesterone was observed at the lowest concentration (3.90 \u03bcM) of FeSO\n                    <jats:sub>4<\/jats:sub>\n                    .7H\n                    <jats:sub>2<\/jats:sub>\n                    O, whereas the lowest release of progesterone by NCIH295R cells was noted after addition of 1000 \u03bcM of FeSO\n                    <jats:sub>4<\/jats:sub>\n                    .7H\n                    <jats:sub>2<\/jats:sub>\n                    O, which did not elicit cytotoxic action (P &gt; 0.05). Testosterone production was substantially increased at the concentrations \u2264 62.50 \u03bcM of FeSO\n                    <jats:sub>4<\/jats:sub>\n                    .7H\n                    <jats:sub>2<\/jats:sub>\n                    O. Lower levels of testosterone were recorded in the groups with higher concentrations (\u2265 250 \u03bcM) of FeSO\n                    <jats:sub>4<\/jats:sub>\n                    .7H\n                    <jats:sub>2<\/jats:sub>\n                    O (P &gt; 0.05). The presented data suggest that iron has no endocrine disruptive effect on the release of sexual steroid hormones, but its toxicity may be reflected at other points of the steroidogenesis pathway.\n                  <\/jats:p>","DOI":"10.14712\/fb2021067020076","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T10:49:55Z","timestamp":1691405395000},"page":"76-81","source":"Crossref","is-referenced-by-count":4,"title":["Human Adrenocortical Carcinoma (NCI-H295R) Cell Line as an In Vitro Cell Culture Model for Assessing the Impact of Iron on Steroidogenesis"],"prefix":"10.14712","volume":"67","author":[{"given":"Z.","family":"K\u0148a\u017eick\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Fialkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"\u010e\u00faranov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Bil\u010d\u00edkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Kov\u00e1\u010dikov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Mi\u0161keje","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Valkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Forg\u00e1cs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Roychoudhury","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Mass\u00e1nyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Luk\u00e1\u010d","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"fb2021067020076_1","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1042\/BJ20140878","volume":"466","author":"Bauckman","year":"2015","journal-title":"Biochem. 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