{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:19:43Z","timestamp":1790623183077,"version":"4.1.0"},"reference-count":30,"publisher":"Charles University in Prague, Karolinum Press","issue":"5-6","license":[{"start":{"date-parts":[[2023,7,30]],"date-time":"2023-07-30T00:00:00Z","timestamp":1690675200000},"content-version":"vor","delay-in-days":940,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,7,30]],"date-time":"2023-07-30T00:00:00Z","timestamp":1690675200000},"content-version":"tdm","delay-in-days":940,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fol. Biol."],"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p>\n                    Apolipoprotein J (clusterin) is a component of high-density lipoproteins, the high level of which is reversely correlated with the risk of coronary heart disease. In addition, it exerts anti-inflammatory and anti-apoptotic effects on endothelial cells and inhibits smooth muscle cell migration and proliferation, indicating that it may play a protective role in cardiovascular disease. However, the exact mechanisms by which this occurs remain unclear. This study aimed to clarify these underlying protective mechanisms by researching the inhibitory effects of apolipoprotein J\n                    <jats:italic>via<\/jats:italic>\n                    the NOD-like receptor protein 3 pathway on the inflammation induced by cholesterol crystals in THP\u20111 macrophages. In culture, THP-1 macrophages were infected with adenoviral vectors containing apolipoprotein J genes and subsequently treated with cholesterol crystals. The inflammatory cytokines interleukin\u20111\u03b2, interleukin 18 and tumour necrosis factor \u03b1 were quantitatively measured with ELISA kits. NOD-like receptor protein 3, cysteinyl aspartate specific proteinase 1 and interleukin 1\u03b2 were evaluated by Western blot and PCR analysis. As a result, apolipoprotein J expression was found to remarkably decrease the levels of inflammatory cytokines, including tumour necrosis factor \u03b1, interleukin 18 and interleukin 1\u03b2, secreted by THP\u20111 macrophages. It was also found capable of inhibiting the levels of NOD-like receptor protein 3, cysteinyl aspartate-specific proteinase 1 and interleukin 1\u03b2 both at the protein and mRNA levels. In the current study, we revealed that over-expression of apolipoprotein J attenuated the inflammation induced by cholesterol crystals through inhibition of the NOD-like receptor protein 3 inflammasome pathway.\n                  <\/jats:p>","DOI":"10.14712\/fb2021067050183","type":"journal-article","created":{"date-parts":[[2023,7,30]],"date-time":"2023-07-30T14:36:21Z","timestamp":1690727781000},"page":"183-190","source":"Crossref","is-referenced-by-count":3,"title":["Over-expression of Apolipoprotein J Inhibits Cholesterol Crystal-Induced Inflammatory Responses via Suppressing NLRP3 Inflammasome Activation in THP-1 Macrophages"],"prefix":"10.14712","volume":"67","author":[{"given":"Y.","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.-Q.","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2023,7,30]]},"reference":[{"key":"fb2021067050183_1","first-page":"2453265","volume":"18","author":"Cacciapaglia","year":"2018","journal-title":"Mediators Inflamm."},{"key":"fb2021067050183_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5551\/jat.2600","volume":"17","author":"Corrado","year":"2010","journal-title":"J. Atheroscler. Thromb."},{"key":"fb2021067050183_3","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.phymed.2013.09.012","volume":"21","author":"Chang","year":"2014","journal-title":"Phytomedicine"},{"key":"fb2021067050183_4","doi-asserted-by":"crossref","first-page":"e8668","DOI":"10.1371\/journal.pone.0008668","volume":"5","author":"Daigneault","year":"2010","journal-title":"PLoS One"},{"key":"fb2021067050183_5","doi-asserted-by":"crossref","first-page":"5380","DOI":"10.1021\/bi00474a025","volume":"29","author":"de Silva","year":"1990","journal-title":"Biochemistry"},{"key":"fb2021067050183_6","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1038\/nature08938","volume":"464","author":"Duewell","year":"2010","journal-title":"Nature"},{"key":"fb2021067050183_7","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1042\/bj3310231","volume":"331","author":"Gelissen","year":"1998","journal-title":"Biochem. J."},{"key":"fb2021067050183_8","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1038\/nrneph.2017.51","volume":"13","author":"Gister\u00e5","year":"2017","journal-title":"Nat. Rev. Nephrol."},{"key":"fb2021067050183_9","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1007\/s10815-012-9779-x","volume":"29","author":"Han","year":"2012","journal-title":"J. Assist. Reprod. Genet."},{"key":"fb2021067050183_10","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1038\/ni.2001","volume":"12","author":"Hansson","year":"2011","journal-title":"Nat. Immunol."},{"key":"fb2021067050183_11","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/j.jaci.2014.05.046","volume":"135","author":"Harden","year":"2015","journal-title":"J. Allergy Clin. Immunol."},{"key":"fb2021067050183_12","doi-asserted-by":"crossref","first-page":"11030","DOI":"10.1016\/S0021-9258(18)99123-4","volume":"266","author":"Jenne","year":"1991","journal-title":"J. Biol. Chem."},{"key":"fb2021067050183_13","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1172\/JCI108450","volume":"58","author":"Katz","year":"1976","journal-title":"J. Clin. Invest."},{"key":"fb2021067050183_14","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.1161\/ATVBAHA.109.190058","volume":"29","author":"Kim","year":"2009","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"fb2021067050183_15","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1159\/000487295","volume":"45","author":"Liu","year":"2018","journal-title":"Cell. Physiol. Biochem."},{"key":"fb2021067050183_16","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"fb2021067050183_17","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1053\/euhj.1998.1350","volume":"20","author":"MRC\/BHF","year":"1999","journal-title":"Eur. Heart J."},{"key":"fb2021067050183_18","doi-asserted-by":"crossref","first-page":"102985","DOI":"10.1016\/j.ebiom.2020.102985","volume":"60","author":"Niyonzima","year":"2020","journal-title":"EBioMedicine"},{"key":"fb2021067050183_19","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s12170-011-0212-2","volume":"6","author":"Ordovas-Montanes","year":"2012","journal-title":"Curr. Cardiovasc. Risk Rep."},{"key":"fb2021067050183_20","first-page":"15","volume":"8","author":"Ozaki","year":"2015","journal-title":"J. Inflamm. Res."},{"key":"fb2021067050183_21","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s11154-013-9275-3","volume":"15","author":"Park","year":"2014","journal-title":"Rev. Endocr. Metab. Disord."},{"key":"fb2021067050183_22","first-page":"537","volume":"22","author":"Poulakou","year":"2008","journal-title":"In Vivo"},{"key":"fb2021067050183_23","doi-asserted-by":"crossref","first-page":"e11765","DOI":"10.1371\/journal.pone.0011765","volume":"5","author":"Rajam\u00e4ki","year":"2010","journal-title":"PLoS One"},{"key":"fb2021067050183_24","doi-asserted-by":"crossref","first-page":"3499","DOI":"10.1093\/eurheartj\/ehy310","volume":"39","author":"Ridker","year":"2018","journal-title":"Eur. Heart J."},{"key":"fb2021067050183_25","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1161\/CIRCULATIONAHA.112.108753","volume":"127","author":"Riwanto","year":"2013","journal-title":"Circulation"},{"key":"fb2021067050183_26","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/S0002-9378(99)70259-0","volume":"180","author":"Staff","year":"1999","journal-title":"Am. J. Obstet. Gynecol."},{"key":"fb2021067050183_27","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1038\/nature10759","volume":"481","author":"Strowig","year":"2012","journal-title":"Nature"},{"key":"fb2021067050183_28","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1111\/j.1365-2362.2010.02345.x","volume":"40","author":"Van Dijk","year":"2010","journal-title":"Eur. J. Clin. Invest."},{"key":"fb2021067050183_29","first-page":"2466","volume":"21","author":"Wang","year":"2020","journal-title":"Mol. Med. Rep."},{"key":"fb2021067050183_30","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1186\/1476-511X-13-70","volume":"13","author":"Yanni","year":"2014","journal-title":"Lipids Health Dis."}],"container-title":["Folia Biologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/foliabiologica.lf1.cuni.cz\/media\/pdf\/fb_2021067050183.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/foliabiologica.lf1.cuni.cz\/media\/pdf\/fb_2021067050183.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:34:29Z","timestamp":1779960869000},"score":1,"resource":{"primary":{"URL":"https:\/\/fb.littledata.cz\/67\/5\/0183\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":30,"journal-issue":{"issue":"5-6","published-online":{"date-parts":[[2021]]}},"alternative-id":["fb_2021067050183"],"URL":"https:\/\/doi.org\/10.14712\/fb2021067050183","relation":{},"ISSN":["0015-5500","2533-7602"],"issn-type":[{"value":"0015-5500","type":"print"},{"value":"2533-7602","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021]]}}}