{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:10:46Z","timestamp":1779963046856,"version":"3.53.1"},"reference-count":28,"publisher":"Charles University in Prague, Karolinum Press","issue":"2","license":[{"start":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T00:00:00Z","timestamp":1701907200000},"content-version":"vor","delay-in-days":340,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T00:00:00Z","timestamp":1701907200000},"content-version":"tdm","delay-in-days":340,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2020R1I1A3074228"],"award-info":[{"award-number":["NRF-2020R1I1A3074228"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007631","name":"Chungnam National University Hospital","doi-asserted-by":"publisher","award":["2013"],"award-info":[{"award-number":["2013"]}],"id":[{"id":"10.13039\/501100007631","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fol. Biol."],"published-print":{"date-parts":[[2023]]},"abstract":"<jats:p>\n                    Although hypothermic treatment has been reported to have some beneficial effects on ischaemia at the clinical level, the mechanism of ischaemia suppression by hypothermia remains unclear due to a lack of mechanism understanding and insufficient data. The aim of this study was to isolate and characterize microRNAs specifically expressed in ischaemia-hypothermia for the dihydropyrimidinase-like 3 (\n                    <jats:italic>Dpysl3<\/jats:italic>\n                    ) gene. PC12 cells were induced with CoCl\n                    <jats:sub>2<\/jats:sub>\n                    for chemical ischaemia and incubated at 32 \u2103 for hypothermia. In ischaemia-hypothermia, four types of microRNAs (miR-106b-5p, miR-194-5p, miR-326-5p, and miR-497-5p) were highly related to the\n                    <jats:italic>Dpysl3<\/jats:italic>\n                    gene based on exosomal microRNA analysis.\n                    <jats:italic>Dpysl3<\/jats:italic>\n                    gene expression was up-regulated by miR-497-5p but down-regulated by miR-106b-5p, miR-194-5p and miR-326-5p. Our results suggest that these four microRNAs are involved in the regulation of\n                    <jats:italic>Dpysl3<\/jats:italic>\n                    gene expression. These findings provide valuable clues that exosomal microRNAs could be used as therapeutic targets for effective treatment of ischaemia.\n                  <\/jats:p>","DOI":"10.14712\/fb2023069020069","type":"journal-article","created":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T04:37:46Z","timestamp":1702010266000},"page":"69-73","source":"Crossref","is-referenced-by-count":2,"title":["Regulation of Dihydropyrimidinase-like 3 Gene Expression by MicroRNAs in PC12 Cells with Induced Ischaemia and Hypothermia"],"prefix":"10.14712","volume":"69","author":[{"given":"Kisang","family":"Kwon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Hye","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyewon","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O-Yu","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Whan","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2023,12,7]]},"reference":[{"key":"fb2023069020069_1","doi-asserted-by":"crossref","first-page":"165260","DOI":"10.1016\/j.bbadis.2018.09.012","volume":"1866","author":"Barthels","year":"2020","journal-title":"Biochim. 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