{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:08:21Z","timestamp":1779962901256,"version":"3.53.1"},"reference-count":43,"publisher":"Charles University in Prague, Karolinum Press","issue":"1","license":[{"start":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T00:00:00Z","timestamp":1717372800000},"content-version":"vor","delay-in-days":154,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T00:00:00Z","timestamp":1717372800000},"content-version":"tdm","delay-in-days":154,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100019054","name":"Changsha Science and Technology Project","doi-asserted-by":"publisher","award":["kq2202002"],"award-info":[{"award-number":["kq2202002"]}],"id":[{"id":"10.13039\/501100019054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fol. Biol."],"published-print":{"date-parts":[[2024]]},"abstract":"<jats:p>\n                    <jats:italic>Chlamydia psittaci<\/jats:italic>\n                    pneumonia (CPP) is a lung disease caused by the infection with the\n                    <jats:italic>Chla\u00admydia psittaci<\/jats:italic>\n                    bacterium, which can lead to severe acute respiratory distress syndrome and systemic symptoms. This study explored the specific mechanisms underlying the impact of reactive oxygen species (ROS) on the Th17\/Treg balance in CPP. The levels of ROS and the differentiation ratio of Th17\/Treg in the peripheral blood of healthy individuals and CPP patients were measured using ELISA and flow cytometry, respectively. The association between the ROS levels and Th17\/Treg was assessed using Pearson correlation analysis. The ROS levels and the Th17\/Treg ratio were measured in CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells following H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    treatment and NLRP3 inhibition. The effects of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    treatment and NLRP3 inhibition on the NLRP3\/IL-1\u03b2\/caspase-1 pathway were observed using immunoblotting. Compared to the healthy group, the CPP group exhibited increased levels of ROS in the peripheral blood, an elevated ratio of Th17 differentiation, and a decreased ratio of Treg differentiation. ROS levels were positively correlated with the Th17 cell proportion but negatively correlated with the Treg cell proportion. The ROS levels and NLRP3\/IL-1\u03b2\/caspase-1 expression were up-regulated in CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells after H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    treatment. Furthermore, there was an increase in Th17 differentiation and a decrease in Treg differentiation. Conversely, the NLRP3\/IL-1\u03b2\/caspase-1 pathway inhibition reversed the effects of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    treatment, with no significant change in the ROS levels. ROS regulates the Th17\/Treg balance in CPP, possibly through the NLRP3\/IL-1\u03b2\/caspase-1 pathway. This study provides a new perspective on the development of immunotherapy for CPP.\n                  <\/jats:p>","DOI":"10.14712\/fb2024070010074","type":"journal-article","created":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T15:30:26Z","timestamp":1717428626000},"page":"74-83","source":"Crossref","is-referenced-by-count":5,"title":["Reactive Oxygen Species Modulate Th17\/Treg Balance in Chlamydia psittaci Pneumonia via NLRP3\/IL-1\u03b2\/Caspase-1 Pathway Differentiation"],"prefix":"10.14712","volume":"70","author":[{"given":"Rong","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianglong","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiguo","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2024,6,3]]},"reference":[{"key":"fb2024070010074_1","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1002\/eji.201747195","volume":"48","author":"Affandi","year":"2018","journal-title":"Eur. 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