{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:15:27Z","timestamp":1779963327095,"version":"3.53.1"},"reference-count":23,"publisher":"Charles University in Prague, Karolinum Press","issue":"4","license":[{"start":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:00:00Z","timestamp":1765411200000},"content-version":"vor","delay-in-days":344,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:00:00Z","timestamp":1765411200000},"content-version":"tdm","delay-in-days":344,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010849","name":"Health and Family Planning Commission of Wuhan Municipality","doi-asserted-by":"publisher","award":["WX23Z01"],"award-info":[{"award-number":["WX23Z01"]}],"id":[{"id":"10.13039\/501100010849","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010849","name":"Health and Family Planning Commission of Wuhan Municipality","doi-asserted-by":"publisher","award":["WX20Q25"],"award-info":[{"award-number":["WX20Q25"]}],"id":[{"id":"10.13039\/501100010849","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fol. Biol."],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p>With a dismal prognosis, cholangiocarcinoma (CCA) is a highly invasive cancer and its global incidence is increasing. Non-coding RNAs, particularly circRNAs, are increasingly recognized as important regulators in tumorigenesis, yet the mechanistic details of hsa_circ_0000977-mediated miR-338-3p regulation in CCA remain incompletely understood. This study aimed to investigate the mechanism of the hsa_circ_0000977\/miR-338-3p\/ETS1 axis in CCA progression, evaluate its prognostic significance and investigate its functional role in CCA cells. Real-time quantitative PCR (RT-qPCR) was performed to quantify hsa_circ_0000977, miR-338-3p and ETS1 mRNA expression levels. Pearson correlation was used to assess their association. To confirm the molecular interactions between these molecules, dual-luciferase reporter assays were employed. Cell migration capacity was evaluated via Transwell migration assays, while CCK-8 tests analysed proliferation. Prognostic value was assessed through survival analysis and multi\u00advariate regression. The study revealed significant up-regulation of hsa_circ_0000977 in CCA tumour tissues, with elevated expression levels associated with poorer 5-year survival outcomes. Multivariate analysis confirmed that hsa_circ_0000977 over-expression is an independent predictor of survival. Functional assays indicated that hsa_circ_0000977 negatively regulated miR-338-3p, which was down-regulated in CCA and exhibited tumour-suppressive effects. CCA cell proliferation and migration were suppressed following hsa_circ_0000977 knockdown, effects that were partially reversed by miR-338-3p inhibition. Further investigation demonstrated that miR-338-3p exerts its tumour-suppressive effects by directly targeting ETS1, and the hsa_circ_0000977\/miR-338-3p\/ETS1 axis regulates the proliferation and migration of CCA cells. We have concluded that hsa_circ_0000977 drives CCA progression by sponging miR-338-3p and modulating its target ETS1, suggesting its clinical value for predicting outcomes and developing targeted therapies.<\/jats:p>","DOI":"10.14712\/fb2025.0002","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T22:42:52Z","timestamp":1765492972000},"page":"162-170","source":"Crossref","is-referenced-by-count":0,"title":["Hsa_circ_0000977 Sponges miR-338-3p to Drive Cholangiocarcinoma Progression and Predict Poor Prognosis"],"prefix":"10.14712","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8204-8907","authenticated-orcid":false,"given":"Shanshan","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3119-8536","authenticated-orcid":false,"given":"Chengcheng","family":"Ying","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8206-2730","authenticated-orcid":false,"given":"Guanbao","family":"Long","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9197-7464","authenticated-orcid":false,"given":"Zouxiao","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4991-9175","authenticated-orcid":false,"given":"Jiangyang","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2025,12,11]]},"reference":[{"key":"fb2025.0002_1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.2147\/IJGM.S186854","volume":"12","author":"Alsaleh","year":"2019","journal-title":"Int. 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