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Biol."],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>\n                    Heat stress disrupts cellular homeostasis in cells by inducing protein misfolding, activating endoplasmic reticulum stress pathways and impairing cell survival mechanisms. The present study aimed to evaluate the cytoprotective effects of tauroursodeoxycholic acid (TUDCA) against heat stress-induced cellular damage in an\n                    <jats:italic>in vitro<\/jats:italic>\n                    spermatogonial GC1 cell model. The IC\n                    <jats:sub>50<\/jats:sub>\n                    concentration of TUDCA was determined by the MTT assay. GC1 cells were divided into four groups: control (GC1C), TUDCA-treated (GC1TUDCA), heat stress model (HSM; GC1HSM) and TUDCA-treated HSM (GC1HSMTUDCA). Standard culture conditions were used for GC1C. HSM group cells were incubated for 60 minutes at 43 \u00b0C. TUDCA groups of cells were treated with 200 \u00b5M TUDCA in culture media for 24 hours. Following the experiments, all groups were subjected to immuno\u00adcytochemical staining with phosphorylated PERK (p-PERK) and C\/EBP homologous protein (CHOP) antibodies, and immunoreactivity was evaluated using the H-Score system. The MTT assay determined the IC\n                    <jats:sub>50<\/jats:sub>\n                    value of TUDCA as 558 \u00b5M for GC1 cells, with 200 \u00b5M selected for subsequent experiments due to minimal cytotoxicity. Heat stress exposure was associated with a reduction in GC1 cell viability and an increase in p-PERK and CHOP expression compared with the GC1C group. In heat-stressed cells, TUDCA treatment was associated with a significant decrease in p-PERK and CHOP expression relative to the GC1HSM group. These results suggest that TUDCA might reduce the cellular reactions related to endoplasmic reticulum stress in heat-stressed GC1 spermatogonial cells under\n                    <jats:italic>in vitro<\/jats:italic>\n                    conditions.\n                  <\/jats:p>","DOI":"10.14712\/fb2026.0007","type":"journal-article","created":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T07:39:56Z","timestamp":1777189196000},"page":"68-74","update-policy":"https:\/\/doi.org\/10.14712\/crossmark-policy-fb","source":"Crossref","is-referenced-by-count":0,"title":["Cytoprotective Effects of Tauroursodeoxycholic Acid in Heat Stress-Induced Endoplasmic Reticulum Stress in Male Germ Cells"],"prefix":"10.14712","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1704-3720","authenticated-orcid":false,"given":"Suna Karadeniz","family":"Sayg\u0131l\u0131","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4662-6493","authenticated-orcid":false,"given":"Orhan","family":"\u00d6zatik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8422-2975","authenticated-orcid":false,"given":"Fikriye Yasemin","family":"\u00d6zatik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5938-3494","authenticated-orcid":false,"given":"Ahmet","family":"Ko\u00e7ak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2466-9417","authenticated-orcid":false,"given":"Ay\u015fe \u00c7ak\u0131r","family":"G\u00fcndo\u011fdu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9914-7122","authenticated-orcid":false,"given":"Mustafa","family":"\u00d6ztatlici","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6627-5443","authenticated-orcid":false,"given":"Mahmut Kemal","family":"Ozbilgin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2026,4,26]]},"reference":[{"key":"fb2026.0007_1","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.matbio.2018.03.015","volume":"68","author":"Burman","year":"2018","journal-title":"Matrix Biol."},{"key":"fb2026.0007_2","doi-asserted-by":"crossref","first-page":"975","DOI":"10.3390\/ani12080975","volume":"12","author":"Capela","year":"2022","journal-title":"Animals (Basel)"},{"key":"fb2026.0007_3","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1038\/s41568-020-00312-2","volume":"21","author":"Chen","year":"2021","journal-title":"Nat. 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