{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:50:19Z","timestamp":1791924619266,"version":"4.3.4"},"update-to":[{"DOI":"10.14712\/fb2026.0017","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000}},{"DOI":"10.14712\/fb2026.0017","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000}}],"reference-count":30,"publisher":"Charles University in Prague, Karolinum Press","issue":"4","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":230,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"tdm","delay-in-days":230,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["foliabiologica.lf1.cuni.cz"],"crossmark-restriction":true},"short-container-title":["Fol. Biol."],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>\n                    Osteoporotic fractures (OF) are the main consequence of osteoporosis. Bone mineral density (BMD)\u2011based diagnosis lacks sensitivity for early osteopoenia (OP) and OF risk. This study evaluated serum miR\u2011642a\u20113p as a diagnostic marker for OP and OF and investigated its mechanism in osteoclast differentiation via\n                    <jats:italic>IFNG<\/jats:italic>\n                    . Bioinformatic analysis of GSE74209 was performed. Serum miR\u2011642a\u20113p levels were measured by RT\u2011qPCR in 30 healthy controls (HCs), 40 OP patients and 40 OF patients. Diagnostic performance, correlation with clinical indicators, and risk prediction for OF were analysed using logistic regression. A dual\u2011luciferase reporter assay confirmed miR\u2011642a\u20113p targeting\n                    <jats:italic>IFNG<\/jats:italic>\n                    . RT\u2011qPCR and Western blot assessed downstream osteoclast markers and the effect of TNF\u2011\u03b1\u2011induced inflammation on miR\u2011642a\u20113p. Serum miR\u2011642a\u20113p was found significantly higher in OP and OF groups than in HCs. The AUC for distinguishing HCs from OP patients was 0.834. miR\u2011642a\u20113p negatively correlated with 25(OH)D and was identified as an independent risk factor for OP. The reporter assay confirmed direct targeting of\n                    <jats:italic>IFNG<\/jats:italic>\n                    by miR\u2011642a\u20113p. Disruption of this axis altered expression of osteoclast markers (NFATc1, CTSK, c\u2011FOS). In the TNF\u2011\u03b1\u2011induced inflammatory model, miR\u2011642a\u20113p increased, IFN-\u03b3 decreased, and osteoclast markers were up-regulated, indicating that miR\u2011642a\u20113p promotes osteoclast differentiation through\n                    <jats:italic>IFNG<\/jats:italic>\n                    suppression. We conclude that the serum miR-642a-3p is a diagnostic biomarker for OP and OF. It promotes osteoclast differentiation by targeting and inhibiting\n                    <jats:italic>IFNG<\/jats:italic>\n                    , identifying a new miR-642a-3p\/IFNG regulatory axis in OP.\n                  <\/jats:p>","DOI":"10.14712\/fb2026.0017","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T12:46:31Z","timestamp":1787143591000},"page":"163-174","update-policy":"https:\/\/doi.org\/10.14712\/crossmark-policy-fb","source":"Crossref","is-referenced-by-count":0,"title":["Serum miR-642a-3p in Osteopoenia and Osteoporotic Fracture: Diagnostic Value for Osteopoenia and Regulation of Osteoclast Differentiation via IFNG"],"prefix":"10.14712","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1006-4282","authenticated-orcid":false,"given":"Sibao","family":"Zeng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6023-0143","authenticated-orcid":false,"given":"Chenhao","family":"Huangfu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8132-0539","authenticated-orcid":false,"given":"Fang","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5575-3401","authenticated-orcid":false,"given":"Yongqi","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5727","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"fb2026.0017_1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s40618-024-02425-z","volume":"48","author":"Arcidiacono","year":"2025","journal-title":"J. Endocrinol. Invest."},{"key":"fb2026.0017_2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.3390\/molecules27010211","volume":"27","author":"Bravo V\u00e1zquez","year":"2021","journal-title":"Molecules"},{"key":"fb2026.0017_3","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1359\/jbmr.061113","volume":"22","author":"Burge","year":"2007","journal-title":"J. Bone Miner. Res."},{"key":"fb2026.0017_4","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/s12020-025-04346-0","volume":"90","author":"Chen","year":"2025","journal-title":"Endocrine"},{"key":"fb2026.0017_5","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s11657-017-0324-5","volume":"12","author":"Compston","year":"2017","journal-title":"Arch. Osteoporos."},{"key":"fb2026.0017_6","doi-asserted-by":"crossref","first-page":"9497","DOI":"10.1002\/jcp.29757","volume":"235","author":"Dinesh","year":"2020","journal-title":"J. Cell. 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