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In conclusion, when using the PI or YPI exclusion assay to determine Wallachian frozen-thawed ram sperm viability, the danger of overestimating the number of spermatozoa with the damaged plasma membrane must be considered. The observed breed-specific, and more importantly, individual differences in gene expression as well as in dye uptake indicate the need for further studies.<\/jats:p>","DOI":"10.14712\/fb2023069040127","type":"journal-article","created":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T04:41:57Z","timestamp":1709008917000},"page":"127-132","source":"Crossref","is-referenced-by-count":1,"title":["A Pilot Study on the Uptake of Propidium Iodide and YO-PRO-1 Iodide through the Pannexin Channels in Wallachian Frozen-Thawed Ram Spermatozoa"],"prefix":"10.14712","volume":"69","author":[{"given":"Filipp Georgijevi\u010d","family":"Savvulidi","sequence":"first","affiliation":[]},{"given":"Martin","family":"Pt\u00e1\u010dek","sequence":"additional","affiliation":[]},{"given":"Ane\u017eka","family":"M\u00e1lkov\u00e1","sequence":"additional","affiliation":[]},{"given":"Elena","family":"Golovina","sequence":"additional","affiliation":[]},{"given":"Jan","family":"Pytl\u00edk","sequence":"additional","affiliation":[]},{"given":"Martina","family":"Jano\u0161\u00edkov\u00e1","sequence":"additional","affiliation":[]},{"given":"Szabolcs","family":"Nagy","sequence":"additional","affiliation":[]},{"given":"Jo\u0103o Pedro","family":"Barbas","sequence":"additional","affiliation":[]},{"given":"Lud\u011bk","family":"St\u00e1dn\u00edk","sequence":"additional","affiliation":[]},{"given":"Karina","family":"Savvulidi Vargov\u00e1","sequence":"additional","affiliation":[]}],"member":"5727","published-online":{"date-parts":[[2024,2,27]]},"reference":[{"key":"fb2023069040127_1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.theriogenology.2017.01.023","volume":"92","author":"Anel-L\u00f3pez","year":"2017","journal-title":"Theriogenology"},{"key":"fb2023069040127_2","first-page":"585","volume":"10","author":"Buchelly","year":"2022","journal-title":"Computer Methods in Biomechanics and Biomedical Engineering: Imaging & Visualization"},{"key":"fb2023069040127_3","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.anireprosci.2015.08.006","volume":"161","author":"David","year":"2015","journal-title":"Anim. 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