{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T02:45:36Z","timestamp":1784601936624,"version":"3.55.0"},"reference-count":0,"publisher":"CMB Association","issue":"2","license":[{"start":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T00:00:00Z","timestamp":1646006400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Mol Biol (Noisy-le-grand)"],"abstract":"<jats:p>This research aimed to investigate the microbial spectrum of microorganisms that cause ventilatorassociated\npneumonia (VAP) among ICU patients in the selected hospital, antimicrobial susceptibility,\ngenetic diversity of common isolates, and the monitoring effect of microbial culture on cleaning and\nsanitizing of external ventilator circuits in order to reduce the occurrence of hospital infections. For this\npurpose, endotracheal aspirate (ETA) specimens were sampled from ICU patients with clinically\nsuspected VAP in the hospital between August 2020 and August 2021 and then investigated for\nmicrobiological content. This was followed by Kirby-Bauer testing for determining drug sensitivity and\nERIC-PCR for genotyping. Afterward, microbial culture was performed on cleaned, sanitized and dried\nventilator external ventilator pipelines and those stored aseptically for 4 weeks to evaluate the cleaning\nand disinfection effect and measure the bacterial content. Results showed that in the 64 confirmed VAP\ncases, Klebsiella was the most frequently isolated organism, followed by P. aeruginosa and\nAcinetobacter baumannii, while Candida is the most widely isolated fungus. The antimicrobial\nsusceptibility spectrum revealed that 40% of the isolates were multidrug-resistant (MDR). ERIC-PCR\nshowed no genetic relationship between pneumococcal isolates. Through microbial culture, no\npathogenic bacteria were detected among cleaned and sanitized ventilator external ventilator pipelines\nand those stored aseptically for 4 weeks, indicating a 100% pass rate. It was concluded that ventilators\nin intensive care units (ICU) are susceptible to contamination, exposing patients to bacterial\ncontamination and other comorbidities. Gram-negative bacteria are the main pathogens of VAP, which\nare mostly multidrug-resistant. Clinical care measures for ventilators should be strengthened to reduce\nthe incidence of ventilator microbial contamination and to improve accurate clinical diagnosis and\ncorrect antimicrobial therapy.<\/jats:p>","DOI":"10.14715\/cmb\/2022.68.2.6","type":"journal-article","created":{"date-parts":[[2022,7,30]],"date-time":"2022-07-30T17:56:21Z","timestamp":1659203781000},"page":"42-47","source":"Crossref","is-referenced-by-count":5,"title":["Monitoring the effect of microbial culture on cleaning and sanitizing of the external\nventilator circuit"],"prefix":"10.14715","volume":"68","author":[{"family":"Fengling Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Feng Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Qi Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Qing Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Linghua  Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5752","published-online":{"date-parts":[[2022,2,28]]},"container-title":["Cellular and Molecular Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.cellmolbiol.org\/index.php\/CMB\/article\/download\/4283\/2043","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.cellmolbiol.org\/index.php\/CMB\/article\/download\/4283\/2043","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,30]],"date-time":"2022-07-30T17:56:42Z","timestamp":1659203802000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cellmolbiol.org\/index.php\/CMB\/article\/view\/4283"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,28]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,6,8]]}},"URL":"https:\/\/doi.org\/10.14715\/cmb\/2022.68.2.6","relation":{},"ISSN":["1165-158X","0145-5680"],"issn-type":[{"value":"1165-158X","type":"electronic"},{"value":"0145-5680","type":"print"}],"subject":[],"published":{"date-parts":[[2022,2,28]]}}}