{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T03:36:29Z","timestamp":1769139389412,"version":"3.49.0"},"reference-count":0,"publisher":"Caleo","issue":"6","license":[{"start":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:00:00Z","timestamp":1763510400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Endod J."],"abstract":"<jats:p>Objective: This study compared the efficacy of continuous chelation with etidronate and sequential chelation with chitosan to traditional chelation with EDTA in reducing bacterial levels in teeth with necrotic pulp.Methods: Sixty patients with single-rooted and single-canalled teeth with pulp necrosis were randomly divided into three groups based on the irrigation protocol with passive ultrasonic activation (PUI). G1 (Control): NaOCl\/EDTA+PUI, G2: NaOCl\/etidronate+PUI, and G3: NaOCl\/chitosan+PUI. Microbiological samples were taken before (S1) and after the root canal preparation and irrigation protocol (S2). Total bacterial counts were measured using 16S ribosomal RNA gene-based real-time quantitative polymerase chain reaction. Statistical analysis was performed using the Wilcoxon signed rank test and the Kruskal-Wallis test (P<\/jats:p>","DOI":"10.14744\/eej.2025.49368","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T11:43:10Z","timestamp":1764848590000},"page":"506-513","source":"Crossref","is-referenced-by-count":0,"title":["Efficacy of Continuous Chelation With Etidronate And Sequential Chelation With Chitosan On Bacterial Reduction In Teeth With Pulp Necrosis: A Double-Blind, Randomized Clinical Trial"],"prefix":"10.65717","volume":"10","author":[{"given":"Karthikeyan","family":"Anbalagan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit","family":"Jena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luna","family":"Samanta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akhil","family":"Changanath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anwesha","family":"Pradhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Govind","family":"Shashirekha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinay","family":"Shivagnage","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"55248","published-online":{"date-parts":[[2025,11,19]]},"container-title":["European Endodontic Journal"],"original-title":[],"link":[{"URL":"https:\/\/www.eurendodj.com\/index.php\/pub\/article\/download\/364\/364","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurendodj.com\/index.php\/pub\/article\/download\/364\/364","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T12:16:24Z","timestamp":1769084184000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurendodj.com\/index.php\/pub\/article\/view\/364"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,19]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,11,19]]}},"URL":"https:\/\/doi.org\/10.14744\/eej.2025.49368","relation":{},"ISSN":["2548-0839"],"issn-type":[{"value":"2548-0839","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,19]]}}}