{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:33:40Z","timestamp":1793144020999,"version":"4.5.0"},"reference-count":0,"publisher":"Kare Publishing","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatol Forum"],"abstract":"<jats:p>Background and Aim: The transcription factor MafF is a novel regulator of adipogenesis, but its role in hepatic steatosis remains unclear. This study aimed to explore the impact of MafF on hepatocyte steatosis and its under-lying mechanisms.\r\nMaterials and Methods: A stable MafF-overexpressing cell line was estab-lished using lentiviral infection. RT-qPCR and Western blot analysis con-firmed MafF expression. Free fatty acid (FFA) or ethanol (ETOH) induction was used to simulate hepatocyte steatosis in non-alcoholic or alcoholic fatty liver disease (NAFLD or AFLD). Cell activity and lipid accumulation were assessed through the CCK-8 assay, Calcein-AM\/PI staining, and Oil Red O staining. The changes in lipid metabolism-related gene expression before and after FFA or ETOH treatment were detected using RT-qPCR.\r\nResults: FFA or ETOH induced lipid accumulation in hepatocytes, and overexpression of MafF significantly ameliorated ETOH-induced hepato-cyte steatosis but had little effect on FFA-induced hepatocyte steatosis. MafF overexpression significantly reduced the expression of peroxisome proliferator-activated receptor gamma (PPARG), acetyl-CoA carboxylase (ACC), and lipoprotein lipase (LPL) in hepatocytes. Upon FFA induc-tion, control (NC) cells exhibited downregulation of these genes, whereas MafF-overexpressing cells upregulated LPL expression. In contrast, under ETOH treatment, NC cells upregulated these genes, while MafF-overex-pressing cells showed downregulation.\r\nConclusion: This study highlighted the regulation of lipid-related genes by MafF, including PPARG, ACC, and LPL, and its effect on FFA- and ETOH-induced hepatocellular lipid accumulation in distinct ways. MafF showed a more pronounced improvement in ETOH-induced hepatocyte ste-atosis, providing crucial insights into MafF\u2019s role in hepatic lipid metabo-lism and potential therapeutic strategies for NAFLD and AFLD.<\/jats:p>","DOI":"10.14744\/hf.2024.2024.0030","type":"journal-article","created":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T03:51:57Z","timestamp":1741319517000},"page":"91-98","source":"Crossref","is-referenced-by-count":1,"title":["The effects of hepatocyte-specific MafF overexpression on FFA or ETOH induced hepatocyte steatosis and its underlying mechanism"],"prefix":"10.14744","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1917-5023","authenticated-orcid":false,"given":"Jinhui","family":"Cai","sequence":"first","affiliation":[{"name":"School of Pharmacy, Guangdong Medical University, Zhanjiang, PR China; 2Pathological Diagnosis and Research Center, Hospital of Guangdong Medical University, Zhanjiang, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0756-1133","authenticated-orcid":false,"given":"Shen","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Guangdong Medical University, Zhanjiang, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8107-3060","authenticated-orcid":false,"given":"Yanmin","family":"Feng","sequence":"additional","affiliation":[{"name":"The First Clinical Medical College, Guangdong Medical University, Zhanjiang, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7856-7608","authenticated-orcid":false,"given":"Lin","family":"Zheng","sequence":"additional","affiliation":[{"name":"The First Clinical Medical College, Guangdong Medical University, Zhanjiang, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6809-3774","authenticated-orcid":false,"given":"Qiuting","family":"Liang","sequence":"additional","affiliation":[{"name":"The First Clinical Medical College, Guangdong Medical University, Zhanjiang, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4208-9413","authenticated-orcid":false,"given":"Yanqing","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Guangdong Medical University, Zhanjiang, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5995-4456","authenticated-orcid":false,"given":"Xiaoxia","family":"Ye","sequence":"additional","affiliation":[{"name":"Pathological Diagnosis and Research Center, Hospital of Guangdong Medical University, Zhanjiang, PR China; Department of Histology and Embryology, Guangdong Medical University, Zhanjiang, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5966","published-online":{"date-parts":[[2025]]},"container-title":["Hepatology Forum"],"original-title":[],"link":[{"URL":"https:\/\/file.paplino.com\/hepatologyforum\/pdfs\/1751889777-en.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/hepatologyforum.org\/storage\/upload\/pdfs\/1751889777-en.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:22:31Z","timestamp":1793143351000},"score":1,"resource":{"primary":{"URL":"https:\/\/hepatologyforum.org\/articles\/HF-11508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.14744\/hf.2024.2024.0030","relation":{},"ISSN":["1307-5888","2757-7392"],"issn-type":[{"value":"1307-5888","type":"print"},{"value":"2757-7392","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]}}}