{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:32:12Z","timestamp":1793143932059,"version":"4.5.0"},"reference-count":0,"publisher":"Kare Publishing","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatol Forum"],"abstract":"<jats:p>Background&amp;Aims: While liver biopsy remains the reference standard for assessing hepatic fibrosis, the major prognostic factor in metabolic dysfunction-associated steatotic liver disease (MASLD), its inherent limitations have driven the search for innovative non-invasive diagnostic tests. In this study, we sought to evaluate the diagnostic accuracy of plasma PDGFR\u00df      and PSD4 methylation levels, integrated within machine learning algorithms, for non-invasive staging of hepatic fibrosis in patients with MASLD, and to compare its performance against established non-invasive biomarkers\r\nMaterials and Methods: Patients with biopsy-proven MASLD and healthy controls were recruited from the three institutions. Quantitative methylation of circulating cell-free DNA was assessed using bisulfite modification and pyrosequencing. The resulting data were used to develop linear discriminant analysis, random forest, and support vector machine algorithms to identify patients at different stages of fibrosis.\r\nResults: The study included 234 patients with histologically confirmed MASLD and 43 healthy controls. Each dataset was validated using an independent cohort. Advanced fibrosis was associated with elevated plasma       PDGFR\u00df and PSD4 methylation levels in both the discovery and validation cohorts.  The integration of plasma DNA methylation markers with clinical parameters demonstrated performance comparable to that of existing noninvasive biomarkers for detecting both significant and advanced fibrosis, achieving area under the curve scores exceeding 0.75 across all models.\r\nConclusions: Supervised machine learning algorithms incorporating plasma PDGFR\u00df and PSD4 DNA methylation levels show promise as a non-invasive approach for assessing hepatic fibrosis in MASLD.<\/jats:p>","DOI":"10.14744\/hf.2025.84568","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T19:03:36Z","timestamp":1779131016000},"page":"125-132","source":"Crossref","is-referenced-by-count":0,"title":["Plasma PDGFR\u00df and PSD4 methylation levels for non-invasive staging of liver fibrosis"],"prefix":"10.14744","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1101-8834","authenticated-orcid":false,"given":"Burge","family":"Ulukan","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology, School of Medicine, Ko\u00e7 University, Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0414-2471","authenticated-orcid":false,"given":"Hong","family":"Yang","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory, KTH- Royal Institute of Technology, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6516-3407","authenticated-orcid":false,"given":"Hande","family":"Uludag","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, School of Medicine, Ko\u00e7 University, Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3721-8586","authenticated-orcid":false,"given":"Cheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory, KTH- Royal Institute of Technology, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1808-7872","authenticated-orcid":false,"given":"Ali","family":"Mutlu","sequence":"additional","affiliation":[{"name":"School of Medicine, Ko\u00e7 University, Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1240-7233","authenticated-orcid":false,"given":"Murat","family":"Dayangac","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Faculty of Medicine, Medipol University, Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2080-7528","authenticated-orcid":false,"given":"Murat","family":"Akyildiz","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, School of Medicine, Ko\u00e7 University, Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6753-2760","authenticated-orcid":false,"given":"Buket","family":"Yigit","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, School of Medicine, Ko\u00e7 University, Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaghayegh","family":"Soleimani","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, School of Medicine, Ko\u00e7 University, Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2968-2610","authenticated-orcid":false,"given":"Hale","family":"Kirimlioglu","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Ac\u0131badem Mehmet Ali Ayd\u0131nlar University, Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2043-2041","authenticated-orcid":false,"given":"Burcu","family":"Saka","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Ko\u00e7 University, Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9360-8116","authenticated-orcid":false,"given":"Cigdem","family":"Ataizi Celikel","sequence":"additional","affiliation":[{"name":"Department of Medical Pathology, School of Medicine, Marmara University, Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7420-3538","authenticated-orcid":false,"given":"Fatih","family":"Eren","sequence":"additional","affiliation":[{"name":"Institute of Gastroenterology, Marmara University, Istanbul, T\u00fcrkiye; Department of Gastroenterology and Hepatology, School of Medicine, Recep Tayyip Erdogan University, Rize, T\u00fcrkiye ; Liver Research Unit, Institute of Gastroenterology, Marmara University, Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4254-6090","authenticated-orcid":false,"given":"Adil","family":"Mardinoglu","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory, KTH- Royal Institute of Technology, Stockholm, Sweden; Centre for Host-Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, King\u2019s College London, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4518-5283","authenticated-orcid":false,"given":"Yusuf","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, School of Medicine, Recep Tayyip Erdogan University, Rize, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1542-117X","authenticated-orcid":false,"given":"Mujdat","family":"Zeybel","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, School of Medicine, Ko\u00e7 University, Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5966","published-online":{"date-parts":[[2026]]},"container-title":["Hepatology Forum"],"original-title":[],"link":[{"URL":"https:\/\/file.paplino.com\/hepatologyforum\/pdfs\/1779128661-en.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/hepatologyforum.org\/storage\/upload\/pdfs\/1779128661-en.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T22:43:09Z","timestamp":1793140989000},"score":1,"resource":{"primary":{"URL":"https:\/\/hepatologyforum.org\/articles\/HF-84568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.14744\/hf.2025.84568","relation":{},"ISSN":["1307-5888","2757-7392"],"issn-type":[{"value":"1307-5888","type":"print"},{"value":"2757-7392","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}