{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T22:28:20Z","timestamp":1778711300400,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2016,7,20]],"date-time":"2016-07-20T00:00:00Z","timestamp":1468972800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aquaporins (<jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s) are transmembrane channel proteins that facilitate rapid water movement across cell membranes. In amniotic membrane, the <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>\u2010facilitated transfer of water across amnion cells has been proposed as a mechanism for amniotic fluid volume (<jats:styled-content style=\"fixed-case\">AFV<\/jats:styled-content>) regulation. To investigate whether <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s modulate <jats:styled-content style=\"fixed-case\">AFV<\/jats:styled-content> by altering intramembranous absorption (<jats:styled-content style=\"fixed-case\">IMA<\/jats:styled-content>) rate, we tested the hypothesis that <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content> gene expression in the amnion is positively correlated with <jats:styled-content style=\"fixed-case\">IMA<\/jats:styled-content> rate during experimental conditions when <jats:styled-content style=\"fixed-case\">IMA<\/jats:styled-content> rate and <jats:styled-content style=\"fixed-case\">AFV<\/jats:styled-content> are modified over a wide range. The relative abundances of <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>3, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>8, <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>9, and <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>11 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein were determined in the amnion of 16 late\u2010gestation ovine fetuses subjected to 2\u00a0days of control conditions, urine drainage, urine replacement, or intraamniotic fluid infusion. <jats:styled-content style=\"fixed-case\">AQP mRNA<\/jats:styled-content> levels were determined by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> and proteins by western immunoblot. Under control conditions, <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels among the five <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s differed more than 20\u2010fold. During experimental treatments, mean <jats:styled-content style=\"fixed-case\">IMA<\/jats:styled-content> rate in the experimental groups ranged from 100\u00a0\u00b1\u00a0120\u00a0<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content>\/day to 1370\u00a0\u00b1\u00a0270\u00a0<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content>\/day. The <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels of the five <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s did not change from control and were not correlated with <jats:styled-content style=\"fixed-case\">IMA<\/jats:styled-content> rates. The protein levels of <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>1 were positively correlated with <jats:styled-content style=\"fixed-case\">IMA<\/jats:styled-content> rates (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a038%, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.01) while the remaining four <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s were not. These findings demonstrate that five <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>s are differentially expressed in ovine amnion. Our study supports the hypothesis that <jats:styled-content style=\"fixed-case\">AQP<\/jats:styled-content>1 may play a positive role in regulating the rate of fluid transfer across the amnion, thereby participating in the dynamic regulation of AFV.<\/jats:p>","DOI":"10.14814\/phy2.12868","type":"journal-article","created":{"date-parts":[[2016,7,21]],"date-time":"2016-07-21T01:33:10Z","timestamp":1469064790000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Aquaporins in ovine amnion: responses to altered amniotic fluid volumes and intramembranous absorption rates"],"prefix":"10.14814","volume":"4","author":[{"given":"Cecilia Y.","family":"Cheung","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Oregon Health and Science University Portland Oregon"},{"name":"Center for Developmental Health Oregon Health and Science University Portland Oregon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debra F.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Center for Developmental Health Oregon Health and Science University Portland Oregon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Brace","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Oregon Health and Science University Portland Oregon"},{"name":"Center for Developmental Health Oregon Health and Science University Portland Oregon"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,7,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2004.12.001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"F463","DOI":"10.1152\/ajprenal.1993.265.4.F463","article-title":"Aquaporin CHIP: the archetypal molecular water channel","volume":"265","author":"Agr P.","year":"1993","journal-title":"Am. 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