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Similarly, <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content> applied to the lateral\u2010line organ (hair cell organ) increases afferent nerve activity in adult frogs (post\u2010metamorphic day 30), yet this increase is developmentally delayed from post\u2010metamorphic day 4\u201330. In this study, we discovered that there was also a developmental delay in increased suprathreshold sound\u2010evoked activity auditory nerve between juvenile and adult mice similar to what had been observed previously in frog. Moreover, juvenile mice with a targeted deletion of the <jats:italic>\u03b1<\/jats:italic><jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content> gene [<jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content> null (\u2212\/\u2212)] did not show a similar developmental increase in nerve activity, suggesting <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content> signaling is involved. This developmental delay is not due to a delay in <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content> expression, but instead is due to a delay in receptor formation. We observed that the increase in sound\u2010evoked nerve activity is correlated with increased formation of cochlear <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content> receptors, which require three complexed proteins (<jats:styled-content style=\"fixed-case\">CLR<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> RAMP<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">RCP<\/jats:styled-content>) to be functional. <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content> receptor formation in the cochlea was incomplete at 1\u00a0month of age (juvenile), but complete by 3\u00a0months (adult), which corresponded to the onset of suprathreshold enhancement of sound\u2010evoked activity in wild\u2010type animals. Taken together, these data support a model for cochlear function that is enhanced by maturation of <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content> receptor complexes.<\/jats:p>","DOI":"10.14814\/phy2.12869","type":"journal-article","created":{"date-parts":[[2016,7,21]],"date-time":"2016-07-21T01:33:10Z","timestamp":1469064790000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Maturation of suprathreshold auditory nerve activity involves cochlear <scp>CGRP<\/scp>\u2013receptor complex formation"],"prefix":"10.14814","volume":"4","author":[{"given":"Ian M.","family":"Dickerson","sequence":"first","affiliation":[{"name":"Deptartment of Neuroscience University of Rochester Medical Center Rochester New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhiannon","family":"Bussey\u2010Gaborski","sequence":"additional","affiliation":[{"name":"Deptartment of Biomedical Engineering University of Rochester Medical Center Rochester New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph C.","family":"Holt","sequence":"additional","affiliation":[{"name":"Deptartment of Otolaryngology University of Rochester Medical Center Rochester New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paivi M.","family":"Jordan","sequence":"additional","affiliation":[{"name":"Deptartment of Otolaryngology University of Rochester Medical Center Rochester New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne E.","family":"Luebke","sequence":"additional","affiliation":[{"name":"Deptartment of Neuroscience University of Rochester Medical Center Rochester New York"},{"name":"Deptartment of Biomedical Engineering University of Rochester Medical Center Rochester New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"5163","DOI":"10.1523\/JNEUROSCI.20-13-05163.2000","article-title":"Calcitonin gene\u2010related peptide suppresses hair cell responses to mechanical stimulation in the Xenopus lateral line organ","volume":"20","author":"Bailey G. 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