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We now analyzed effects of the soluble mediators released into the coronary arterial blood during stent implantation on vasomotion of isolated rat epicardial coronary artery segments and on coronary flow and left ventricular developed pressure in isolated perfused rat hearts. Coronary blood was retrieved during percutaneous <jats:styled-content style=\"fixed-case\">SVG<\/jats:styled-content> intervention using a distal occlusion\/aspiration protection device in nine symptomatic patients with stable angina pectoris and a flow\u2010limiting <jats:styled-content style=\"fixed-case\">SVG<\/jats:styled-content> stenosis. The blood was separated into particulate debris and plasma. Responses to coronary plasma were determined in isolated rat epicardial coronary arteries and in isolated, constant pressure\u2010perfused rat hearts (\u00b1nitric oxide synthase [<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content>] inhibition and \u00b1serotonin receptor blockade, respectively). Coronary aspirate plasma taken after stent implantation induced a stronger vasoconstriction of rat epicardial coronary arteries\u00a0(52\u00a0\u00b1\u00a08% of maximal potassium chloride induced vasoconstriction [% <jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content>l<jats:sub>max<\/jats:sub>\u00a0=\u00a0100%]) than plasma taken before stent implantation (12\u00a0\u00b1\u00a08% of <jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content>l<jats:sub>max<\/jats:sub>); <jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content> inhibition augmented this vasoconstrictor response (to 110\u00a0\u00b1\u00a015% and 24\u00a0\u00b1\u00a09% of <jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content>l<jats:sub>max<\/jats:sub>). Coronary aspirate plasma taken after stent implantation reduced in isolated perfused rat hearts only under <jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content> inhibition coronary flow by 17\u00a0\u00b1\u00a03% and left ventricular developed pressure by 25\u00a0\u00b1\u00a04%. Blockade of serotonin receptors abrogated these effects. Coronary aspirate plasma taken after stent implantation induces vasoconstriction in isolated rat epicardial coronary arteries and reduces coronary flow and left ventricular developed pressure in isolated perfused rat hearts with pharmacologically induced endothelial dysfunction.<\/jats:p>","DOI":"10.14814\/phy2.12874","type":"journal-article","created":{"date-parts":[[2016,8,2]],"date-time":"2016-08-02T01:18:26Z","timestamp":1470100706000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Aspirate from human stented saphenous vein grafts induces epicardial coronary vasoconstriction and impairs perfusion and left ventricular function in rat bioassay hearts with pharmacologically induced endothelial dysfunction"],"prefix":"10.14814","volume":"4","author":[{"given":"Helmut R.","family":"Lieder","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Pathophysiologie Universit\u00e4tsklinikum Essen Westdeutsches Herz\u2010 und Gef\u00e4\u00dfzentrum Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodor","family":"Baars","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Kardiologie Universit\u00e4tsklinikum Essen Westdeutsches Herz\u2010 und Gef\u00e4\u00dfzentrum Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Kahlert","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Kardiologie Universit\u00e4tsklinikum Essen Westdeutsches Herz\u2010 und Gef\u00e4\u00dfzentrum Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Kleinbongard","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pathophysiologie Universit\u00e4tsklinikum Essen Westdeutsches Herz\u2010 und Gef\u00e4\u00dfzentrum Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"e166","article-title":"ACC\/AHA\/SCAI 2005 guideline update for percutaneous coronary intervention: a report of the American College of Cardiology\/American Heart Association Task Force on Practice Guidelines","volume":"113","author":"ACC\/AHA\/SCAI Writing Committee to Update 2001 Guidelines for Percutaneous Coronary Intervention","year":"2006","journal-title":"Circulation"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2014.06.1170"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-012-0303-3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.89.2.623"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2011.02.018"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(83)90597-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCINTERVENTIONS.114.001403"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/24.11.932"},{"key":"e_1_2_7_10_1","first-page":"H529","article-title":"Effects of atherosclerosis on the coronary microcirculation","volume":"258","author":"Chilian W. 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