{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T04:58:50Z","timestamp":1789707530096,"version":"4.0.1"},"reference-count":29,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2016,11,8]],"date-time":"2016-11-08T00:00:00Z","timestamp":1478563200000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000106","name":"Saskatchewan Health Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000106","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002715","name":"Alberta Livestock and Meat Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002715","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">MAM<\/jats:styled-content> domain\u2010containing 4 (<jats:styled-content style=\"fixed-case\">MAMDC<\/jats:styled-content>4) protein is associated with the unique endocytotic mechanism observed in the intestine of mammals during the immediate postnatal period. Transcriptional expression of <jats:styled-content style=\"fixed-case\">MAMDC<\/jats:styled-content>4 was substantially upregulated at birth in both the piglet jejunum and ileum and its expression decreases after birth. The protein was found localized specifically to the apical region of the luminal epithelium, however, <jats:styled-content style=\"fixed-case\">MAMDC<\/jats:styled-content>4 protein expression was lost at day 10 and 15 in the jejunum and ileum, respectively, and was not associated with \u201cfetal\u201d enterocyte replacement. Although spatial variation in the subcellular localization of Claudin 1 (<jats:styled-content style=\"fixed-case\">CLDN<\/jats:styled-content>1) was noted at day 3, the loss of <jats:styled-content style=\"fixed-case\">MAMDC<\/jats:styled-content>4 at later stages of development did not appear to have any effect on the tight junction structure. Germ\u2010free (<jats:styled-content style=\"fixed-case\">GF<\/jats:styled-content>) piglets and piglets whose gastrointestinal flora consists exclusively of <jats:italic>Escherichia coli<\/jats:italic> (<jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content>) or <jats:italic>Lactobacillus fermentum<\/jats:italic> (<jats:styled-content style=\"fixed-case\">LF<\/jats:styled-content>) maintained <jats:styled-content style=\"fixed-case\">MAMDC<\/jats:styled-content>4 protein expression to 14\u00a0days of age in distal regions of the small intestine whereas those with conventionalized intestinal flora (<jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>) showed no <jats:styled-content style=\"fixed-case\">MAMDC<\/jats:styled-content>4 protein at this age. <jats:styled-content style=\"fixed-case\">MAMDC<\/jats:styled-content>4 protein expression was most pronounced in the <jats:styled-content style=\"fixed-case\">LF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GF<\/jats:styled-content> colonized piglets which showed staining in the epithelial cells at 75% and 95% of the length of the small intestine, respectively, which matched that of the newborn. In contrast, <jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content> animals showed only a low abundance at these regions as well as a discontinuous staining pattern. Collectively these results suggest that maturation of <jats:styled-content style=\"fixed-case\">MAMDC<\/jats:styled-content>4 expression in the porcine epithelium occurs more rapidly than what is reported in previously studied rodent species. Furthermore, intestinal bacterial colonization is a major regulator of <jats:styled-content style=\"fixed-case\">MAMDC<\/jats:styled-content>4 in a manner specific to bacterial species and independent of enterocyte turnover.<\/jats:p>","DOI":"10.14814\/phy2.13018","type":"journal-article","created":{"date-parts":[[2016,11,8]],"date-time":"2016-11-08T04:14:15Z","timestamp":1478578455000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Postnatal regulation of <scp>MAMDC<\/scp>4 in the porcine intestinal epithelium is influenced by bacterial colonization"],"prefix":"10.14814","volume":"4","author":[{"given":"Alex J.","family":"Pasternak","sequence":"first","affiliation":[{"name":"Vaccine and Infectious Disease Organization International Vaccine Centre (VIDO\u2010InterVac) University of Saskatchewan Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenn M.","family":"Hamonic","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Organization International Vaccine Centre (VIDO\u2010InterVac) University of Saskatchewan Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Van Kessel","sequence":"additional","affiliation":[{"name":"Department of Animal and Poultry Science University of Saskatchewan Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather L.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Organization International Vaccine Centre (VIDO\u2010InterVac) University of Saskatchewan Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3300367"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(83)90236-X"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/42.2.7507141"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-8-215"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr070038b"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2001.20706.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1955.0068"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M110.005231"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.12.3259"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.229202. Article published online before March 2002"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1093\/jn\/70.1.13","article-title":"Porcine neonatal nutrition: the effect of diet on blood serum proteins and performance of the baby pig","volume":"70","author":"Lecce J. G.","year":"1960","journal-title":"J. Nutr."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2010.01052.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000677"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2011.03.022"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/263629"},{"key":"e_1_2_7_18_1","first-page":"37","volume-title":"Milk Production. Food Sciences and Technology","author":"Salmon H.","year":"2012"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2008.07.007"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/153537020623100807"},{"issue":"3","key":"e_1_2_7_21_1","first-page":"87","article-title":"Gradual disappearance of vacuolated enterocytes in the small intestine of neonatal piglets","volume":"58","author":"Skrzypek T.","year":"2007","journal-title":"J. Physiol. Pharmacol."},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1985.5"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1978.0092"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005176-198605020-00024"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-7-research0034"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2007-0320"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0396.2008.00841.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.2.691"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-6226(00)00217-7"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2005.08.001"}],"container-title":["Physiological Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.14814%2Fphy2.13018","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.13018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T05:40:27Z","timestamp":1696484427000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.14814\/phy2.13018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11]]},"references-count":29,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.14814\/phy2.13018"],"URL":"https:\/\/doi.org\/10.14814\/phy2.13018","archive":["Portico"],"relation":{},"ISSN":["2051-817X","2051-817X"],"issn-type":[{"value":"2051-817X","type":"print"},{"value":"2051-817X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11]]},"assertion":[{"value":"2016-09-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-10-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13018"}}