{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T21:33:16Z","timestamp":1779917596234,"version":"3.53.1"},"reference-count":60,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,2,13]],"date-time":"2017-02-13T00:00:00Z","timestamp":1486944000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["PG\/15\/66\/31710"],"award-info":[{"award-number":["PG\/15\/66\/31710"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cardioprotective efficacy of remote ischemic preconditioning (<jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content>) remains controversial. Experimental studies investigating <jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content> have largely monitored cardiovascular changes during index ischemia and reperfusion with little work investigating changes during <jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content> application. This work aims to identify cardiovascular changes associated with autonomic nervous system (<jats:styled-content style=\"fixed-case\">ANS<\/jats:styled-content>) activity during <jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content> and prior to index ischemia. <jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content> was induced in anesthetized male C57\/Bl6 mice by four cycles of 5\u00a0min of hindlimb ischemia using inflated cuff (200\u00a0mmHg) followed by 5\u00a0min reperfusion. Electrocardiography (<jats:styled-content style=\"fixed-case\">ECG<\/jats:styled-content>) and microcirculatory blood flow in both hindlimbs were recorded throughout <jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content> protocol. Heart rate variability (<jats:styled-content style=\"fixed-case\">HRV<\/jats:styled-content>) analysis was performed using <jats:styled-content style=\"fixed-case\">ECG<\/jats:styled-content> data. Hearts extracted at the end of <jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content> protocol were used either for measurement of myocardial metabolites using high\u2010performance liquid chromatography or for Langendorff perfusion to monitor function and injury during 30\u00a0min index ischemia and 2\u00a0h reperfusion. Isolated\u2010perfused hearts from <jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content> animals had significantly less infarct size after index ischemia and reperfusion (34\u00a0\u00b1\u00a05% vs. 59\u00a0\u00b1\u00a07%; mean\u00a0\u00b1\u00a0<jats:styled-content style=\"fixed-case\">SE <\/jats:styled-content><jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). <jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content> protocol was associated with increased heart rate measured both in ex\u00a0vivo and in\u00a0vivo. Frequency ratio of <jats:styled-content style=\"fixed-case\">HRV<\/jats:styled-content> spectra was altered in <jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content> compared to control. <jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content> was associated with a standard hyperemic response in the cuffed\u2010limb but there was a sustained reduction in blood flow in the uncuffed contralateral limb. <jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content> hearts (prior to index ischemia) had significantly lower phosphorylation potential and energy charge compared to the control group. In conclusion, <jats:styled-content style=\"fixed-case\">RIPC<\/jats:styled-content> is associated with changes in <jats:styled-content style=\"fixed-case\">ANS<\/jats:styled-content> activity (heart rate, blood flow, <jats:styled-content style=\"fixed-case\">HRV<\/jats:styled-content>) and mild myocardial ischemic stress that would contribute to cardioprotection.<\/jats:p>","DOI":"10.14814\/phy2.13085","type":"journal-article","created":{"date-parts":[[2017,2,14]],"date-time":"2017-02-14T01:10:23Z","timestamp":1487034623000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Remote ischemic preconditioning triggers changes in autonomic nervous system activity: implications for cardioprotection"],"prefix":"10.14814","volume":"5","author":[{"given":"Safa","family":"Abdul\u2010Ghani","sequence":"first","affiliation":[{"name":"Bristol Heart Institute School of Clinical Sciences Faculty of Medicine &amp; Dentistry University of Bristol Bristol United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold N.","family":"Fleishman","sequence":"additional","affiliation":[{"name":"Research Institute for Complex Problems of Hygiene and Occupational Diseases Novokuznetsk Kemerovo Oblast Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Khaliulin","sequence":"additional","affiliation":[{"name":"Bristol Heart Institute School of Clinical Sciences Faculty of Medicine &amp; Dentistry University of Bristol Bristol United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Meloni","sequence":"additional","affiliation":[{"name":"BHF Centre for Cardiovascular Science University of Edinburgh Edinburgh United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianni D.","family":"Angelini","sequence":"additional","affiliation":[{"name":"Bristol Heart Institute School of Clinical Sciences Faculty of Medicine &amp; Dentistry University of Bristol Bristol United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u2010Saadeh","family":"Suleiman","sequence":"additional","affiliation":[{"name":"Bristol Heart Institute School of Clinical Sciences Faculty of Medicine &amp; Dentistry University of Bristol Bristol United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,13]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1","article-title":"Cardiac energetics during remote ischemic preconditioning","volume":"18","author":"Abdul\u2010Ghani S.","year":"2013","journal-title":"Exp. 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