{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T10:27:26Z","timestamp":1769164046166,"version":"3.49.0"},"reference-count":69,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T00:00:00Z","timestamp":1484870400000},"content-version":"am","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T00:00:00Z","timestamp":1484870400000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000154","name":"Division of Integrative Organismal Systems","doi-asserted-by":"publisher","award":["1354181"],"award-info":[{"award-number":["1354181"]}],"id":[{"id":"10.13039\/100000154","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM067550"],"award-info":[{"award-number":["GM067550"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005384","name":"American Physiological Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005384","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The enzyme soluble adenylyl cyclase (sAC) is directly stimulated by bicarbonate (HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>) to produce the signaling molecule cyclic adenosine monophosphate (cAMP). Because sAC and sAC\u2010related enzymes are found throughout phyla from cyanobacteria to mammals and they regulate cell physiology in response to internal and external changes in pH, CO<jats:sub>2<\/jats:sub>, and HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>, sAC is deemed an evolutionarily conserved acid\u2010base sensor. Previously, sAC has been reported in dogfish shark and round ray gill cells, where they sense and counteract blood alkalosis by regulating the activity of V\u2010type H<jats:sup>+<\/jats:sup>\u2010 ATPase. Here, we report the presence of sAC protein in gill, rectal gland, cornea, intestine, white muscle, and heart of leopard shark<jats:italic>Triakis semifasciata<\/jats:italic>. Co\u2010expression of sAC with transmembrane adenylyl cyclases supports the presence of cAMP signaling microdomains. Furthermore, immunohistochemistry on tissue sections, and western blots and cAMP\u2010activity assays on nucleus\u2010enriched fractions demonstrate the presence of sAC protein in and around nuclei. These results suggest that sAC modulates multiple physiological processes in shark cells, including nuclear functions.<\/jats:p>","DOI":"10.14814\/phy2.13090","type":"journal-article","created":{"date-parts":[[2017,1,21]],"date-time":"2017-01-21T01:25:45Z","timestamp":1484961945000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Bicarbonate\u2010sensing soluble adenylyl cyclase is present in the cell cytoplasm and nucleus of multiple shark tissues"],"prefix":"10.14814","volume":"5","author":[{"given":"Jinae N.","family":"Roa","sequence":"first","affiliation":[{"name":"Marine Biology Research Division Scripps Institution of Oceanography University of California San Diego 9500 Gilman Drive La Jolla California 92093 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Tresguerres","sequence":"additional","affiliation":[{"name":"Marine Biology Research Division Scripps Institution of Oceanography University of California San Diego 9500 Gilman Drive La Jolla California 92093 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,1,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/299161a0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000113749"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.1.79"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000172"},{"key":"e_1_2_8_6_1","first-page":"1002","article-title":"Electrical parameters of the isolated cornea of the dogfish","volume":"15","author":"Candia A.","year":"1976","journal-title":"Squalus acanthias. Invest Ophthalmol"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00360-012-0685-4"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5479.625"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.08.032"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20031061"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbd.2013.09.003"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2014.08.016"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbd.2007.11.003"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400050101"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003251"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00584.2004"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00645.2009"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M805501200"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-73336-9_8"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2005.06.007"},{"key":"e_1_2_8_21_1","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780195117028.001.0001","volume-title":"Biochemical Adaptation","author":"Hochachka P. W.","year":"2002"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2014.07.027"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201512075"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00317.2013"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00551.2012"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03364.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2014.07.032"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2014.03.008"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0944-5_6"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00314.2010"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01905"},{"key":"e_1_2_8_32_1","first-page":"662","article-title":"The rates of ion movement from plasma to aqueous humor in the dogfish","volume":"14","author":"Maren T. H.","year":"1975","journal-title":"Squalus acanthias. Invest Ophthalmol"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2014.05.011"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00406.2007"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00584.2009"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00360-003-0354-8"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00089.2016"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2014.04.003"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)33555-X"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.113.02061"},{"key":"e_1_2_8_41_1","first-page":"201","article-title":"Forskolin: a unique diterpene activator of cyclic AMP\u2010generating systems","volume":"7","author":"Seamon K. B.","year":"1981","journal-title":"J Cyclic Nucleotide Res"},{"key":"e_1_2_8_42_1","first-page":"11","article-title":"Corneal hydration. Comparative physiology of fish and mammals","volume":"1","author":"Smelser G. K.","year":"1962","journal-title":"Invest Ophthalmol"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00035.2006"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401990319"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22926"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6793-4-8"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009050477"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00507.2014"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2014.06.021"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.128389"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.01382"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02059"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00814.2006"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-010-0865-6"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00761.2009"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911790107"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2011.95"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.086157"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260419"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02329"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.138198"},{"key":"e_1_2_8_62_1","volume-title":"Fish physiology: the multifunctional gut of fish","author":"Wood C. M.","year":"2011"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.01678"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02736"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2006.09.003"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1086\/587094"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2009.09.006"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0598fje"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200311119"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2009.440"}],"container-title":["Physiological Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.14814%2Fphy2.13090","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.13090","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.14814\/phy2.13090","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/chorus\/v1\/articles\/10.14814%2Fphy2.13090","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.13090","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/full\/10.14814\/phy2.13090","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/full\/10.14814\/phy2.13090","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T23:36:41Z","timestamp":1719013001000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.14814\/phy2.13090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1]]},"references-count":69,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.14814\/phy2.13090"],"URL":"https:\/\/doi.org\/10.14814\/phy2.13090","archive":["Portico"],"relation":{},"ISSN":["2051-817X","2051-817X"],"issn-type":[{"value":"2051-817X","type":"print"},{"value":"2051-817X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1]]},"assertion":[{"value":"2016-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-01-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13090"}}