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For example, it can trigger formation of advanced glycation end (<jats:styled-content style=\"fixed-case\">AGE<\/jats:styled-content>) products with ischemia\u2010reperfusion performed under hyperglycemic conditions. For this study, we hypothesized that albumin modified by glycation loses its unique cardioprotective properties in the setting of ischemia\u2010reperfusion under high glucose conditions. Here, ex\u00a0vivo rat heart perfusions were performed under simulated normo\u2010 and hyperglycemic conditions, that is Krebs\u2010Henseleit buffer containing 11\u00a0mmol\/L and 33\u00a0mmol\/L glucose, respectively, \u00b1 normal or glycated albumin preparations. The perfusion protocol consisted of a 60\u00a0min stabilization step that was followed by 20\u00a0min of global ischemia and 60\u00a0min reperfusion. Additional experiments were completed to determine infarct sizes in response to 20\u00a0min regional ischemia and 120\u00a0min reperfusion. At the end of perfusions, heart tissues were isolated and evaluated for activation of the <jats:styled-content style=\"fixed-case\">AGE<\/jats:styled-content> pathway, oxidative stress, and apoptosis. Our data reveal that native bovine serum albumin treatment elicited cardioprotection (improved functional recovery, decreased infarct sizes) under high glucose conditions together with enhanced myocardial antioxidant capacity. However, such protective features are lost with glycation where hearts displayed increased infarct sizes and poor functional recovery versus native albumin treatments. Myocardial antioxidant capacity was also lowered together with activation of the intracellular <jats:styled-content style=\"fixed-case\">AGE<\/jats:styled-content> pathway. These data therefore show that although albumin acts as a cardioprotective agent during ischemia\u2010reperfusion, it loses its cardioprotective and antioxidant properties when modified by glycation.<\/jats:p>","DOI":"10.14814\/phy2.13107","type":"journal-article","created":{"date-parts":[[2017,1,27]],"date-time":"2017-01-27T01:10:37Z","timestamp":1485479437000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Glycation abolishes the cardioprotective effects of albumin during ex\u00a0vivo ischemia\u2010reperfusion"],"prefix":"10.14814","volume":"5","author":[{"given":"Rudo F.","family":"Mapanga","sequence":"first","affiliation":[{"name":"Cardio\u2010Metabolic Research Group (CMRG) Department of Physiological Sciences Stellenbosch University Stellenbosch South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danzil E.","family":"Joseph","sequence":"additional","affiliation":[{"name":"Cardio\u2010Metabolic Research Group (CMRG) Department of Physiological Sciences Stellenbosch University Stellenbosch South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4218-515X","authenticated-orcid":false,"given":"Marco","family":"Saieva","sequence":"additional","affiliation":[{"name":"Cardio\u2010Metabolic Research Group (CMRG) Department of Physiological Sciences Stellenbosch University Stellenbosch South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Boyer","sequence":"additional","affiliation":[{"name":"Inserm UMR 1188 Diab\u00e8te ath\u00e9rothrombose Th\u00e9rapies R\u00e9union Oc\u00e9an Indien (D\u00e9TROI) Universit\u00e9 de La R\u00e9union Plateforme CYROI Saint Denis de La R\u00e9union France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Rondeau","sequence":"additional","affiliation":[{"name":"Inserm UMR 1188 Diab\u00e8te ath\u00e9rothrombose Th\u00e9rapies R\u00e9union Oc\u00e9an Indien (D\u00e9TROI) Universit\u00e9 de La R\u00e9union Plateforme CYROI Saint Denis de La R\u00e9union France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Bourdon","sequence":"additional","affiliation":[{"name":"Inserm UMR 1188 Diab\u00e8te ath\u00e9rothrombose Th\u00e9rapies R\u00e9union Oc\u00e9an Indien (D\u00e9TROI) Universit\u00e9 de La R\u00e9union Plateforme CYROI Saint Denis de La R\u00e9union France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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