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Rodent models and human epidemiological studies have linked this chemical to neurobehavior impairments. In California mice, developmental exposure to BPA results in sociosexual disorders at adulthood, including communication and biparental care deficits, behaviors that are primarily regulated by the hypothalamus. Thus, we sought to examine the transcriptomic profile in this brain region of juvenile male and female California mice offspring exposed from periconception through lactation to BPA or ethinyl estradiol (EE, estrogen present in birth control pills and considered a positive estrogen control for BPA studies). Two weeks prior to breeding, P<jats:sub>0<\/jats:sub> females were fed a control diet, or this diet supplemented with 50\u00a0mg BPA\/kg feed weight or 0.1 ppb EE, and continued on the diets through lactation. At weaning, brains from male and female offspring were collected, hypothalamic RNA isolated, and RNA\u2010seq analysis performed. Results indicate that BPA and EE groups clustered separately from controls with BPA and EE exposure leading to unique set of signature gene profiles. <jats:italic>Kcnd3<\/jats:italic> was downregulated in the hypothalamus of BPA\u2010 and EE\u2010exposed females, whereas <jats:italic>Tbl2<\/jats:italic>,<jats:italic> Topors<\/jats:italic>,<jats:italic> Kif3a<\/jats:italic>, and <jats:italic>Phactr2<\/jats:italic> were upregulated in these groups. Comparison of transcripts differentially expressed in BPA and EE groups revealed significant enrichment of gene ontology terms associated with microtubule\u2010based processes. Current results show that perinatal exposure to BPA or EE can result in several transcriptomic alterations, including those associated with microtubule functions, in the hypothalamus of California mice. It remains to be determined whether these genes mediate BPA\u2010induced behavioral disruptions.<\/jats:p>","DOI":"10.14814\/phy2.13133","type":"journal-article","created":{"date-parts":[[2017,2,15]],"date-time":"2017-02-15T01:10:20Z","timestamp":1487121020000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Hypothalamic transcriptomic alterations in male and female California mice (<i>Peromyscus californicus<\/i>) developmentally exposed to bisphenol A or ethinyl estradiol"],"prefix":"10.14814","volume":"5","author":[{"given":"Sarah A.","family":"Johnson","sequence":"first","affiliation":[{"name":"Bond Life Sciences Center University of Missouri Columbia Missouri"},{"name":"Biomedical Sciences University of Missouri Columbia Missouri"},{"name":"Animal Sciences University of Missouri Columbia Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William G.","family":"Spollen","sequence":"additional","affiliation":[{"name":"Bond Life Sciences Center University of Missouri Columbia Missouri"},{"name":"Informatics Research Core Facility University of Missouri Columbia Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lindsey K.","family":"Manshack","sequence":"additional","affiliation":[{"name":"Bond Life Sciences Center University of Missouri Columbia Missouri"},{"name":"Biomedical Sciences University of Missouri Columbia Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan J.","family":"Bivens","sequence":"additional","affiliation":[{"name":"DNA Core Facility University of Missouri Columbia Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott A.","family":"Givan","sequence":"additional","affiliation":[{"name":"Bond Life Sciences Center University of Missouri Columbia Missouri"},{"name":"Informatics Research Core Facility University of Missouri Columbia Missouri"},{"name":"Molecular Microbiology and Immunology University of Missouri Columbia Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl S.","family":"Rosenfeld","sequence":"additional","affiliation":[{"name":"Bond Life Sciences Center University of Missouri Columbia Missouri"},{"name":"Biomedical Sciences University of Missouri Columbia Missouri"},{"name":"Genetics Area Program University of Missouri Columbia Missouri"},{"name":"Thompson Center for Autism and Neurobehavioral Disorders University of Missouri Columbia Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,14]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Lukasz Komsta2011.Outliers: Tests for outliers. 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