{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:36:21Z","timestamp":1787614581597,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T00:00:00Z","timestamp":1489449600000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Monoclonal antibody therapies targeting the <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> receptor (<jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content>) frequently result in hypomagnesemia in human patients. In contrast, <jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content> tyrosine kinase inhibitors do not affect Mg<jats:sup>2+<\/jats:sup> balance in patients and only have a mild effect on Mg<jats:sup>2+<\/jats:sup> homeostasis in rodents at elevated doses. <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> has also been shown to affect phosphate (P<jats:sub>i<\/jats:sub>) transport in rat and rabbit proximal convoluted tubules (<jats:styled-content style=\"fixed-case\">PCT<\/jats:styled-content>), but evidence from studies targeting <jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content> and looking at P<jats:sub>i<\/jats:sub> excretion in whole animals is still missing. Thus, the role of <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> in regulating reabsorption of Mg<jats:sup>2+<\/jats:sup> and\/or P<jats:sub>i<\/jats:sub> in the kidney remains controversial. Here, we inject mice with the anti\u2010<jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content> monoclonal antibody <jats:styled-content style=\"fixed-case\">ME<\/jats:styled-content>\u20101 for 2\u00a0weeks and observe a significant increase in serum P<jats:sub>i<\/jats:sub> and mild hypomagnesemia, but no changes in P<jats:sub>i<\/jats:sub> or Mg<jats:sup>2+<\/jats:sup> excretion. In contrast, a single injection of <jats:styled-content style=\"fixed-case\">ME<\/jats:styled-content>\u20101 resulted in hyperphosphatemia and a significant reduction in P<jats:sub>i<\/jats:sub> excretion 2\u00a0days after treatment, while no changes in serum Mg<jats:sup>2+<\/jats:sup> or Mg<jats:sup>2+<\/jats:sup> excretion were observed. Dietary Mg<jats:sup>2+<\/jats:sup> deprivation is known to trigger a rapid Mg<jats:sup>2+<\/jats:sup> conservation response in addition to hyperphosphatemia and hyperphosphaturia. Interestingly, one dose of <jats:styled-content style=\"fixed-case\">ME<\/jats:styled-content>\u20101 did not significantly modify the response of mice to 2\u00a0days of Mg<jats:sup>2+<\/jats:sup> deprivation. These data show that <jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content> plays a significant role in regulating P<jats:sub>i<\/jats:sub> reabsorption in the kidney <jats:styled-content style=\"fixed-case\">PCT<\/jats:styled-content>, but suggest only a minor role in long\u2010term regulation of Mg<jats:sup>2+<\/jats:sup> transport in the distal convoluted tubule.<\/jats:p>","DOI":"10.14814\/phy2.13176","type":"journal-article","created":{"date-parts":[[2017,3,15]],"date-time":"2017-03-15T00:10:18Z","timestamp":1489536618000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antibody\u2010mediated inhibition of EGFR reduces phosphate excretion and induces hyperphosphatemia and mild hypomagnesemia in mice"],"prefix":"10.14814","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3473-7778","authenticated-orcid":false,"given":"Bernardo","family":"Ortega","sequence":"first","affiliation":[{"name":"Department of Biology The College at Brockport State University of New York Brockport New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason M.","family":"Dey","sequence":"additional","affiliation":[{"name":"Department of Biology The College at Brockport State University of New York Brockport New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison R.","family":"Gardella","sequence":"additional","affiliation":[{"name":"Department of Biology The College at Brockport State University of New York Brockport New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Proano","sequence":"additional","affiliation":[{"name":"Department of Biology The College at Brockport State University of New York Brockport New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deanna","family":"Vaneerde","sequence":"additional","affiliation":[{"name":"Department of Biology The College at Brockport State University of New York Brockport New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/dji412"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gft358"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.276.1.F72"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1988.255.2.C155"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2009.11.004"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009111153"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2016.09.010"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"S101","DOI":"10.1684\/mrh.2011.0289","article-title":"Role of the distal convoluted tubule in renal Mg2\u00a0+\u00a0 handling: molecular lessons from inherited hypomagnesemia","volume":"24","author":"Ferre S.","year":"2011","journal-title":"Magnes. 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