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In the latter, voltage\u2010induced Ca<jats:sup>2+<\/jats:sup>release (VICR) is regarded as the dominant mechanism facilitating contractions. Any role of the SOICR in the regulation of cytoplasmic Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) and its dynamics in skeletal muscle in\u00a0vivo remains poorly understood. By means of in\u00a0vivo single fiber Ca<jats:sup>2+<\/jats:sup>microinjections combined with bioimaging techniques, we tested the hypothesis that the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>dynamics following Ca<jats:sup>2+<\/jats:sup>injection would be amplified and fiber contraction facilitated by SOICR. The circulation\u2010intact spinotrapezius muscle of adult male Wistar rats (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>34) was exteriorized and loaded with Fura\u20102 AM to monitor [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>dynamics. Groups of rats underwent the following treatments: (1) 0.02, 0.2, and 2.0\u00a0mmol\/L Ca<jats:sup>2+<\/jats:sup>injections, (2) 2.0\u00a0mmol\/L Ca<jats:sup>2+<\/jats:sup>with inhibition of ryanodine receptors (RyR) by dantrolene sodium (DAN), and (3) 2.0\u00a0mmol\/L Ca<jats:sup>2+<\/jats:sup>with inhibition of SR Ca<jats:sup>2+<\/jats:sup>ATPase (SERCA) by cyclopiazonic acid (CPA). A quantity of 0.02\u00a0mmol\/L Ca<jats:sup>2+<\/jats:sup>injection yielded no detectable response, whereas peak evoked [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>increased 9.9\u00a0\u00b1\u00a01.8% above baseline for 0.2\u00a0mmol\/L and 23.8\u00a0\u00b1\u00a04.3% (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) for 2.0\u00a0mmol\/L Ca<jats:sup>2+<\/jats:sup>injections. The peak [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in response to 2.0\u00a0mmol\/L Ca<jats:sup>2+<\/jats:sup>injection was largely abolished by DAN and CPA (\u221285.8%, \u221271.0%, respectively, both<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05 vs. unblocked) supporting dependence of the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>dynamics on Ca<jats:sup>2+<\/jats:sup>released by SOICR rather than injected Ca<jats:sup>2+<\/jats:sup>itself. Thus, this investigation demonstrates the presence of a robust SR\u2010evoked SOICR operant in skeletal muscle in\u00a0vivo.<\/jats:p>","DOI":"10.14814\/phy2.13180","type":"journal-article","created":{"date-parts":[[2017,3,15]],"date-time":"2017-03-15T00:10:18Z","timestamp":1489536618000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["In vivo Ca<sup>2+<\/sup>dynamics induced by Ca<sup>2+<\/sup>injection in individual rat skeletal muscle fibers"],"prefix":"10.14814","volume":"5","author":[{"given":"Mario","family":"Wakizaka","sequence":"first","affiliation":[{"name":"Department of Engineering Science, Bioscience and Technology Program University of Electro\u2010Communications Chofu Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Eshima","sequence":"additional","affiliation":[{"name":"Department of Engineering Science, Bioscience and Technology Program University of Electro\u2010Communications Chofu Tokyo Japan"},{"name":"Department of Metabolism and Endocrinology Juntendo University Graduate School of Medicine Hongo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Engineering Science, Bioscience and Technology Program University of Electro\u2010Communications Chofu Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Shirakawa","sequence":"additional","affiliation":[{"name":"Department of Engineering Science, Bioscience and Technology Program University of Electro\u2010Communications Chofu Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David C.","family":"Poole","sequence":"additional","affiliation":[{"name":"Departments of Anatomy &amp; Physiology and Kinesiology Kansas State University Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Kano","sequence":"additional","affiliation":[{"name":"Department of Engineering Science, Bioscience and Technology Program University of Electro\u2010Communications Chofu Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.t01-1-00657.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/390493"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2003.00125.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201010461"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/2044-5040-1-18"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3440"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.274274"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.003bo.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1253\/circj.CJ-09-0478"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00040.2008"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/228034a0"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00023.2013"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-3362-7_18"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.225854"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00013.2002"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.146.3.621"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16011066"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.128.5.893"},{"key":"e_1_2_6_20_1","first-page":"420","article-title":"Critical role of intracellular RyR1 calcium release channels in skeletal muscle function and disease","volume":"6","author":"Hernandez\u2010Ochoa E. 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