{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T07:56:51Z","timestamp":1792051011350,"version":"4.4.0"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,3,21]],"date-time":"2017-03-21T00:00:00Z","timestamp":1490054400000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["RO1 DK 097268"],"award-info":[{"award-number":["RO1 DK 097268"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["P30DK42086"],"award-info":[{"award-number":["P30DK42086"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["T32 DK007074"],"award-info":[{"award-number":["T32 DK007074"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chronic diseases arise when there is mutual reinforcement of pathophysiological processes that cause an aberrant steady state. Such a sequence of events may underlie chronic constipation, which has been associated with dysbiosis of the gut. In this study we hypothesized that assemblage of microbial communities, directed by slow gastrointestinal transit, affects host function in a way that reinforces constipation and further maintains selection on microbial communities. In our study, we used two models \u2013 an opioid\u2010induced constipation model in mice, and a humanized mouse model where germ\u2010free mice were colonized with stool from a patient with constipation\u2010predominant irritable bowel syndrome (IBS\u2010C) in humans. We examined the impact of pharmacologically (loperamide)\u2010induced constipation (PIC) and IBS\u2010C on the structural and functional profile of the gut microbiota. Germ\u2010free (GF) mice were colonized with microbiota from PIC donor mice and IBS\u2010C patients to determine how the microbiota affects the host. PIC and IBS\u2010C promoted changes in the gut microbiota, characterized by increased relative abundance of <jats:italic>Bacteroides ovatus<\/jats:italic> and <jats:italic>Parabacteroides distasonis<\/jats:italic> in both models. PIC mice exhibited decreased luminal concentrations of butyrate in the cecum and altered metabolic profiles of the gut microbiota. Colonization of GF mice with PIC\u2010associated mice cecal or human IBS\u2010C fecal microbiota significantly increased GI transit time when compared to control microbiota recipients. IBS\u2010C\u2010associated gut microbiota also impacted colonic contractile properties. Our findings support the concept that constipation is characterized by disease\u2010associated steady states caused by reinforcement of pathophysiological factors in host\u2010microbe interactions.<\/jats:p>","DOI":"10.14814\/phy2.13182","type":"journal-article","created":{"date-parts":[[2017,3,21]],"date-time":"2017-03-21T01:40:41Z","timestamp":1490060441000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Mutual reinforcement of pathophysiological host\u2010microbe interactions in intestinal stasis models"],"prefix":"10.14814","volume":"5","author":[{"given":"Ketrija","family":"Touw","sequence":"first","affiliation":[{"name":"Department of Medicine University of Chicago Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daina L.","family":"Ringus","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Chicago Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel","family":"Hubert","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Chicago Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Chicago Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa A.","family":"Leone","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Chicago Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuradha","family":"Nadimpalli","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Chicago Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betty R.","family":"Theriault","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Chicago Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong E.","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Chicago Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johnathan D.","family":"Tune","sequence":"additional","affiliation":[{"name":"Enteric NeuroScience Program Division of Gastroenterology and Hepatology Mayo Clinic Rochester Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul B.","family":"Herring","sequence":"additional","affiliation":[{"name":"Department of Cellular and Integrative Physiology Indiana University School of Medicine Indianapolis Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianrico","family":"Farrugia","sequence":"additional","affiliation":[{"name":"Enteric NeuroScience Program Division of Gastroenterology and Hepatology Mayo Clinic Rochester Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Purna C.","family":"Kashyap","sequence":"additional","affiliation":[{"name":"Enteric NeuroScience Program Division of Gastroenterology and Hepatology Mayo Clinic Rochester Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dionysios A.","family":"Antonopoulos","sequence":"additional","affiliation":[{"name":"Institute for Genomics and Systems Biology Argonne National Laboratory Argonne Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene B.","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Chicago Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2005.00230.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2012.05007.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.01.053"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2015.0954"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041594"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2004.04114.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-020711-074937"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12425"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.280.3.G368"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2011-301501"},{"key":"e_1_2_7_13_1","first-page":"G427","article-title":"Short\u2010chain fatty acids stimulate motility of the canine ileum","volume":"253","author":"Kamath P. 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