{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T11:16:29Z","timestamp":1784718989243,"version":"3.55.0"},"reference-count":54,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2017,11,17]],"date-time":"2017-11-17T00:00:00Z","timestamp":1510876800000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Spanish Ministry of Economy and Competitiveness","award":["2014-55399"],"award-info":[{"award-number":["2014-55399"]}]},{"name":"The Spanish Ministry of Economy and Competitiveness","award":["SAF2016-77222-R"],"award-info":[{"award-number":["SAF2016-77222-R"]}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fetal\/perinatal hypoxia is one of the most common causes of perinatal morbidity and mortality and is frequently accompannied by vascular dysfunction. However, the mechanisms involved have not been fully delineated. We hypothesized that exposure to acute hypoxia\u2010reoxygenation induces alterations in vascular O<jats:sub>2<\/jats:sub> sensing\/signaling as well as in endothelial function in the chicken embryo pulmonary artery (PA), mesenteric artery (MA), femoral artery (FA), and ductus arteriosus (DA). Noninternally pipped 19\u2010day embryos were exposed to 10% O<jats:sub>2<\/jats:sub> for 30\u00a0min followed by reoxygenation with 21% O<jats:sub>2<\/jats:sub> or 80% O<jats:sub>2<\/jats:sub>. Another group was constantly maintained at 21% O<jats:sub>2<\/jats:sub> or at 21% O<jats:sub>2<\/jats:sub> for 30\u00a0min and then exposed to 80% O<jats:sub>2<\/jats:sub>. Following treatment, responses of isolated blood vessels to hypoxia as well as endothelium\u2010dependent (acetylcholine) and \u2010independent (sodium nitroprusside and forskolin) relaxation were investigated in a wire myograph. Hypoxia increased venous blood lactate from 2.03\u00a0\u00b1\u00a00.18 to 15.98\u00a0\u00b1\u00a00.73\u00a0mmol\/L (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) and reduced hatchability to 0%. However, ex\u00a0vivo hypoxic contraction of PA and MA, hypoxic relaxation of FA, and normoxic contraction of DA were not significantly different in any of the experimental groups. Relaxations induced by acetylcholine, sodium nitroprusside, and forskolin in PA, MA, FA, and DA rings were also similar in the four groups. In conclusion, exposure to acute hypoxia\u2010reoxygenation did not affect vascular oxygen sensing or reactivity in the chicken embryo. This suggests that direct effects of acute hypoxia\u2010reoxygenation on vascular function does not play a role in the pathophysiology of hypoxic cardiovascular injury in the perinatal period.<\/jats:p>","DOI":"10.14814\/phy2.13501","type":"journal-article","created":{"date-parts":[[2017,11,17]],"date-time":"2017-11-17T01:10:18Z","timestamp":1510881018000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Acute hypoxia\u2010reoxygenation and vascular oxygen sensing in the chicken embryo"],"prefix":"10.14814","volume":"5","author":[{"given":"Riazuddin","family":"Mohammed","sequence":"first","affiliation":[{"name":"Department of Pediatrics Maastricht University Medical Center (MUMC+) School for Oncology and Developmental Biology (GROW) Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos E.","family":"Salinas","sequence":"additional","affiliation":[{"name":"Instituto Boliviano de Biolog\u00eda de Altura Facultad de Medicina Universidad Mayor de San Andr\u00e9s La Paz Bolivia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dino A.","family":"Giussani","sequence":"additional","affiliation":[{"name":"Department of Physiology Development &amp; Neuroscience University of Cambridge Cambridge United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos E.","family":"Blanco","sequence":"additional","affiliation":[{"name":"Department of Neonatology National Maternity Hospital Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel L.","family":"Cogolludo","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine Universidad Complutense de Madrid Centro de Investigaciones Biom\u00e9dicas en Red de Enfermedades Respiratorias (CIBERES) Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n (IiSGM) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1949-9123","authenticated-orcid":false,"given":"Eduardo","family":"Villamor","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Maastricht University Medical Center (MUMC+) School for Oncology and Developmental Biology (GROW) Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00204.2006"},{"key":"e_1_2_7_3_1","first-page":"55","article-title":"Developmental changes in endothelium\u2010dependent relaxation of the chicken ductus arteriosus","volume":"59","author":"Agren P.","year":"2008","journal-title":"J. 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