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While the functional and structural adaptations to this overload are known, the adaptation of myocardial mechanical properties remains unknown. Using a rodent model of MR, in this study, we discern changes in the passive material properties of the intact and decellularized myocardium. Eighty Sprague\u2010Dawley rats (350\u2013400\u00a0g) were assigned to two groups: (1) MR (<jats:italic>n<\/jats:italic>\u00a0=\u00a040) and (2) control (<jats:italic>n<\/jats:italic>\u00a0=\u00a040). MR was induced in the beating heart by perforating the mitral leaflet with a 23G needle, and rats were terminated at 2, 10, 20, or 40\u00a0weeks (<jats:italic>n<\/jats:italic>\u00a0=\u00a010\/time\u2010point). Echocardiography was performed at baseline and termination, and explanted hearts were used for equibiaxial mechanical testing of the intact myocardium and after decellularization. Two weeks after inducing severe MR, the myocardium was more extensible compared to control, however, stiffness and extensibility of the extracellular matrix did not differ from control at this timepoint. By 20\u00a0weeks, the myocardium was stiffer with a higher elastic modulus of 1920\u00a0\u00b1\u00a0246\u00a0kPa, and a parallel rise in extracellular matrix stiffness. Despite some matrix stiffening, it only contributed to 31% and 36% of the elastic modulus of the intact tissue in the circumferential and longitudinal directions. At 40\u00a0weeks, similar trends of increasing stiffness were observed, but the contribution of extracellular matrix remained relatively low. Chronic MR induces ventricular myocardial stiffening, which seems to be driven by the myocyte compartment of the muscle, and not the extracellular matrix.<\/jats:p>","DOI":"10.14814\/phy2.15305","type":"journal-article","created":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T05:19:56Z","timestamp":1658726396000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Passive mechanical properties of the left ventricular myocardium and extracellular matrix in hearts with chronic volume overload from mitral regurgitation"],"prefix":"10.14814","volume":"10","author":[{"given":"Daniella","family":"Corporan","sequence":"first","affiliation":[{"name":"Structural Heart Research and Innovation Laboratory Carlyle Fraser Heart Center Emory University Hospital Midtown  Atlanta Georgia USA"},{"name":"Division of Cardiothoracic Surgery Department of Surgery Emory University School of Medicine  Atlanta Georgia 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