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Elevated Pi and IDA both lead to increased FGF23, however, the roles of iron and FGF23 during CKD remain unclear. To this end, iron and Pi metabolism were tested in a mouse model of CKD (0.2% adenine)\u00a0\u00b1\u00a00.5% FC for 6\u00a0weeks, with and without osteocyte deletion of <jats:italic>Fgf23<\/jats:italic> (flox\u2010Fgf23\/Dmp1\u2010Cre). Intact FGF23 (iFGF23) increased in all CKD mice but was lower in Cre<jats:sup>+<\/jats:sup> mice with or without FC, thus the Dmp1\u2010Cre effectively reduced FGF23. Cre<jats:sup>+<\/jats:sup> mice fed AD\u2010only had higher serum Pi than Cre<jats:sup>\u2212<\/jats:sup> pre\u2010 and post\u2010diet, and the Cre<jats:sup>+<\/jats:sup> mice had higher BUN regardless of FC treatment. Total serum iron was higher in all mice receiving FC, and liver Tfrc, Bmp6, and hepcidin mRNAs were increased regardless of genotype; liver IL\u20106 showed decreased mRNA in FC\u2010fed mice. The renal 1,25\u2010dihydroxyvitamin D (1,25D) anabolic enzyme Cyp27b1 had higher mRNA and the catabolic Cyp24a1 showed lower mRNA in FC\u2010fed mice. Finally, mice with loss of FGF23 had higher bone cortical porosity, whereas Raman spectroscopy showed no changes in matrix mineral parameters. Thus, FC\u2010 and FGF23\u2010dependent and \u2010independent actions were identified in CKD; loss of FGF23 was associated with higher serum Pi and BUN, demonstrating that FGF23 was protective of mineral metabolism. In contrast, FC maintained serum iron and corrected inflammation mediators, potentially providing ancillary benefit.<\/jats:p>","DOI":"10.14814\/phy2.15307","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T07:19:49Z","timestamp":1654240789000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Segregating the effects of ferric citrate\u2010mediated iron utilization and FGF23 in a mouse model of CKD"],"prefix":"10.14814","volume":"10","author":[{"given":"Michael P.","family":"Liesen","sequence":"first","affiliation":[{"name":"Department of Medical &amp; Molecular Genetics Indiana University School of Medicine  Indianapolis Indiana USA"},{"name":"Department of Physiology Marian University  Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1763-1867","authenticated-orcid":false,"given":"Megan 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